A Novel Mathematical Model for Studying Antimicrobial Interactions Against Campylobacter jejuni

Hakeem, Mohammed Jamal; Asseri, Khalid A.; Ma, Luyao; Chou, Kuang-Chao; Konkel, Michael E.; Lu, Xiaonan

doi:10.3389/fmicb.2019.01038

Cited by 8 publications

(5 citation statements)

References 29 publications

(44 reference statements)

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“…The 96-well microtiter plate was incubated in an orbital shaker under microaerobic conditions at 37°C for 48 h. After incubation, the optical density was determined using a 96-well plate reader (BioTek Instruments Inc., Winooski, VT) at 595 nm (OD 595 ). The concentrations and absorbance values were graphed on a logarithmic plot using GraphPad Prism (v6.0.h, La Jolla, CA), and the IC 50 was calculated from the concentration-effect curve as described previously ( Hakeem et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

The bile salt deoxycholate induces Campylobacter jejuni genetic point mutations that promote increased antibiotic resistance and fitness

Talukdar

Crockett²,

Gloss³

et al. 2022

Front. Microbiol.

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Oxidative damage to DNA is a significant source of mutations in living organisms. While DNA damage must be repaired to maintain the integrity of the genome and cell survival, errors made during DNA repair may contribute to evolution. Previous work has revealed that Campylobacter jejuni growth in the presence of bile salt deoxycholate (DOC) causes an increase in reactive oxygen species and the occurrence of 8-oxo-deoxyguanosine (8-oxo-dG) DNA lesions. The fundamental goal of this project was to determine if C. jejuni growth in a medium containing DOC contributes to DNA mutations that provide a fitness advantage to the bacterium. Co-culture experiments revealed that C. jejuni growth in a DOC-supplemented medium increases the total number of ciprofloxacin-resistant isolates compared to C. jejuni grown in the absence of DOC. We recovered two individual isolates grown in a medium with DOC that had a point mutation in the gene encoding the EptC phosphoethanolamine transferase. Transformants harboring the EptC variant protein showed enhanced resistance to the antimicrobial agent polymyxin B and DOC when compared to an eptC deletion mutant or the isolate complemented with a wild-type copy of the gene. Finally, we found that the base excision repair (BER), homologous recombination repair (HRR), and nucleotide excision repair (NER) are involved in general oxidative damage repair in C. jejuni but that the BER pathway plays the primary role in the repair of the 8-oxo-dG lesion. We postulate that bile salts drive C. jejuni mutations (adaptations) and enhance bacterial fitness in animals.

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Section: Methodsmentioning

confidence: 99%

The bile salt deoxycholate induces Campylobacter jejuni genetic point mutations that promote increased antibiotic resistance and fitness

Talukdar

Crockett²,

Gloss³

et al. 2022

Front. Microbiol.

Self Cite

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“…Nonetheless, the evaluation of synergism requires accurate methods to study drug interaction, considering potential differences between the dose-response relationship of individual drugs and avoiding over-or under-estimation of interactionS. For example, while the time-kill curve of C. jejuni treated with both cinnamon oil and ZnO NPs resulted in the over-estimation of synergism between the antimicrobials, the fractional inhibitory concentration index (FICI) method showed no synergism but only an additive effect [233]. The FICI method was not able to detect the synergism between binary combinations of antimicrobials (cinnamon oil, ZnO NPs, and CUR encapsulated in starch) at sub-MIC, which resulted in the non-turbidity of C. jejuni.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…In turn, mathematical modeling using isobolograms and median-effect curves showed synergism when CUR in starch was combined with other antimicrobials against C. jejuni, with bacterial reductions of 3 log for the binary combination and over 8 log for the tertiary combination. The mathematical modeling suggested that CUR in starch was the main antimicrobial responsible for the synergistic interaction [233].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review

Trigo-Gutierrez

Vega-Chacón

Soares

et al. 2021

IJMS

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Curcumin (CUR) is a natural substance extracted from turmeric that has antimicrobial properties. Due to its ability to absorb light in the blue spectrum, CUR is also used as a photosensitizer (PS) in antimicrobial Photodynamic Therapy (aPDT). However, CUR is hydrophobic, unstable in solutions, and has low bioavailability, which hinders its clinical use. To circumvent these drawbacks, drug delivery systems (DDSs) have been used. In this review, we summarize the DDSs used to carry CUR and their antimicrobial effect against viruses, bacteria, and fungi, including drug-resistant strains and emergent pathogens such as SARS-CoV-2. The reviewed DDSs include colloidal (micelles, liposomes, nanoemulsions, cyclodextrins, chitosan, and other polymeric nanoparticles), metallic, and mesoporous particles, as well as graphene, quantum dots, and hybrid nanosystems such as films and hydrogels. Free (non-encapsulated) CUR and CUR loaded in DDSs have a broad-spectrum antimicrobial action when used alone or as a PS in aPDT. They also show low cytotoxicity, in vivo biocompatibility, and improved wound healing. Although there are several in vitro and some in vivo investigations describing the nanotechnological aspects and the potential antimicrobial application of CUR-loaded DDSs, clinical trials are not reported and further studies should translate this evidence to the clinical scenarios of infections.

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“…The method was based on previous experiments with some modifications ( Xiao et al, 2015 ; Hakeem et al, 2019 ). The time-killing curve test was conducted to verify the time it took to kill all the bacteria.…”

Section: Green-route Synthesis Of Agnpsmentioning

confidence: 99%

Green synthesis of silver nanoparticles through oil: Promoting full-thickness cutaneous wound healing in methicillin-resistant Staphylococcus aureus infections

Wang

Peng

et al. 2022

Front. Bioeng. Biotechnol.

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Due to the emergence of multi-drug resistant microorganisms, the development and discovery of alternative eco-friendly antimicrobial agents have become a top priority. In this study, a simple, novel, and valid green method was developed to synthesize Litsea cubeba essential oil-silver nanoparticles (Lceo-AgNPs) using Lceo as a reducing and capping agent. The maximum UV absorbance of Lceo-AgNPs appeared at 423 nm and the size was 5–15 nm through transmission electron microscopy result. The results of Fourier transform infrared and DLS showed that Lceo provided sufficient chemical bonds for Lceo-AgNPs to reinforce its stability and dispersion. The in vitro antibacterial effects of Lceo-AgNPs against microbial susceptible multidrug-resistant Escherichia coli (E. coli) and methicillin-resistant Staphylococcus aureus (MRSA) were determined. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Lceo-AgNPs against E. coli were 25 and 50 μg/ml. The MIC and MBC of Lceo-AgNPs against MRSA were 50 and 100 μg/ml, respectively. The results of scanning electron microscopy showed that the amount of bacteria obviously decreased and the bacteria cells were destroyed by Lceo-AgNPs. In vivo research disclosed significant wound healing and re-epithelialization effects in the Lceo-AgNPs group compared with the self-healing group and the healing activity was better than in the sulfadiazine silver group. In this experiment, Lceo-AgNPs has been shown to have effects on killing multidrug-resistant bacteria and promoting wound healing. This study suggested Lceo-AgNPs as an excellent new-type drug for wound treatment infected with multidrug-resistant bacteria, and now expects to proceed with clinical research.

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A Novel Mathematical Model for Studying Antimicrobial Interactions Against Campylobacter jejuni

Cited by 8 publications

References 29 publications

The bile salt deoxycholate induces Campylobacter jejuni genetic point mutations that promote increased antibiotic resistance and fitness

The bile salt deoxycholate induces Campylobacter jejuni genetic point mutations that promote increased antibiotic resistance and fitness

Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review

Green synthesis of silver nanoparticles through oil: Promoting full-thickness cutaneous wound healing in methicillin-resistant Staphylococcus aureus infections

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