A novel membrane-based process to isolate peroxidase from horseradish roots: optimization of operating parameters

Liu, Jianguo; Yang, Bo; Chen, Changzhen

doi:10.1007/s00449-012-0781-6

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…The purification factor decreased as CaCl 2 concentration was further increased, reaching values of 2.5 at the maximum concentration utilized (10 mmol L −1 ). Similar results were reported by Liu et al ., who verified that Ca +2 increased the horseradish peroxidase specific activity only for CaCl 2 concentrations below 10 mmol L −1 .…”

Section: Resultssupporting

confidence: 91%

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Gautério

Malta

Reginatto

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Once peroxidase purification can improve substrate degradation capacity, peroxidase from rice bran was purified by expanded bed adsorption (EBA) coupled to diafiltration/ultrafiltration (DF/UF) and applied for deoxynivalenol (DON) reduction. RESULTS The DF/UF step was optimized according to membrane molecular weight cutoff (MWCO), DF cycles number, operation temperature and CaCl2 addition to the DF buffer. DON reduction tests were carried out with both clarified and purified peroxidase. The use of MWCO of 10 kDa, 10 °C, six DF cycles and adding 4 mmol L−1 CaCl2 to DF buffer promoted enzyme purification of 4.4‐fold and enzymatic recovery of 79.4%. The global purification process combining two techniques resulted in enzyme purification of 75.1‐fold with an enzymatic recovery of 22.8%. Peroxidase purified by EBA combined with DF/UF resulted in DON reduction of 81.7% in a model system. CONCLUSION Peroxidase purification was achieved by scale‐up integrated strategies. The use of purified peroxidase from agricultural by‐products could be a promising alternative for enzymatic DON levels reduction. © 2017 Society of Chemical Industry

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Section: Resultssupporting

confidence: 91%

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Gautério

Malta

Reginatto

et al. 2017

J of Chemical Tech & Biotech

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Horseradish peroxidase delignification of fermented rooibos modifies biomass structural and chemical properties and improves holocellulolytic enzyme cocktail efficacy

Mohotloane,

Alexander,

Pletschke

et al. 2023

Biologia

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The purpose of the study was to pretreat fermented rooibos biomass with partially purified horseradish peroxidase (HRP) for lignin removal and to convert delignified biomass to soluble sugars through saccharification with a formulated holocellulolytic enzyme cocktail (HEC). HRP enzyme was extracted from the horseradish root tissue and was partially purified by membrane filters and characterised biochemically. HRP enzyme was used to pretreat the fermented rooibos biomass to remove lignin before hydrolysing it with the HEC. Our findings indicated that HRP is versatile because it displayed activity on guaiacol, 8-aminoquinoline, and decolourised methylene blue dye. HRP had a pH optimum of 4.5 and displayed a mesophilic temperature range. The kinetics studies indicated that HRP displayed a higher affinity towards guaiacol (Km= 0.082 mg/mL) followed by 8-aminoquinoline (Km= 0.221 mg/mL). However, the catalytic efficiency revealed that the enzyme hydrolysed guaiacol (63436.48 s− 1. mg/mL) and 8-aminoquinoline (59189.81 s− 1. mg/mL) efficiently. HRP pretreatment of rooibos biomass significantly removed lignin content and increased pores on the surface as visualised with SEM. FTIR validated the SEM results by showing reductions at 3324.81, 1615.16 and 1018.75 cm− 1, corresponding to crystalline cellulose, lignin and holocellulose regions, respectively. HRP pretreated biomass had the lowest crystallinity index of 11.2% compared to 20% of the control. HRP delignified rooibos biomass was hydrolysed effectively by the HEC, which released about 10% yield of soluble sugars compared to 6% of control. We conclude that HRP pretreatment significantly modified the structural and chemical properties of the biomass, making it more accessible to hydrolytic enzymes.

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A bifunctional AuNP probe-based enzyme-linked immunosorbent assay for facile and ultrasensitive detection of trace zearalenone in coix seed

Huang

et al. 2023

Microchemical Journal

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A novel membrane-based process to isolate peroxidase from horseradish roots: optimization of operating parameters

Cited by 3 publications

References 18 publications

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Horseradish peroxidase delignification of fermented rooibos modifies biomass structural and chemical properties and improves holocellulolytic enzyme cocktail efficacy

A bifunctional AuNP probe-based enzyme-linked immunosorbent assay for facile and ultrasensitive detection of trace zearalenone in coix seed

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