A novel membrane-type apatite scaffold engineered by pulsed laser ablation

Abstract

Cell sheet technology is a scaffold-free method for tissue reconstruction. A sheet-shaped scaffold would be suitable for the regeneration of periodontal membrane. We designed a stem cell sheet combining human mesenchymal stromal cells (hMSCs) and a 10-µm thick biological apatite (BAp) membrane fabricated with an ArF pulsed laser ablation for periodontal regeneration. X-ray diffraction showed that crystalline hydroxyapatite (HAp) was present in BAp and HAp membranes after post-annealing. Energy dispersive analysis of the BAp membrane revealed peaks of Na and Mg in addition to Ca and P. Approximately 3×10 4 hMSCs were cultured on BAp and HAP membranes for 7 and 14 days. From in vitro assays, hMSCs grew faster and had higher osteoblast differentiation when cultured on the BAp membrane than did the cell culture on the HAp membrane. Stem cell sheets combined with a BAp membrane may have potential applications in guided bone regeneration and osteoconductive scaffolds.

Hydroxyapatite Film Coating by Er:YAG Pulsed Laser Deposition Method for the Repair of Enamel Defects

“…To simulate the hydrolysis of α-TCP under intraoral conditions, the bovine tooth slabs were kept in artificial saliva composed of multiple ions at a temperature of 37 °C after Er:YAG-PLD. Peaks of sodium and magnesium ions have also been detected on the bovine enamel surface [ 38 , 39 ]. However, it is clear that the Ca/P value of bovine enamel and HAp coating is lower than 1.67 that of hydroxyapatite crystals.…”

Application of fluoridated hydroxyapatite thin film coatings using KrF pulsed laser deposition

“…Specifically, HA films induce cell attachment and proliferation 29) due to the adsorption of a high quantity of fibronectin 30,31) . Our previous study 32) demonstrated that HMSCs grew faster and exhibited higher osteoblast differentiation compared with pure titanium when cultured on HA film coatings applied using PLD. In the present study, the FHA film had a significant impact on cell attachment of HMSCs compared with HA film.…”

Periodontal Ligament Stem Cells for Periodontal Regeneration

“…The form of a scaffold is influenced by the defect site and characteristics of tissues to be None None regenerated; films, sheets, sponges, particles, colloids, and gel types are currently used. Furthermore, various materials have been used to develop scaffolds including collagen sponges [38,75], gelatin sponges [38,76], bone materials [77,78], alginate hydrogels [79,80], biodegradable polyesters [81][82][83][84], polydopamine [85], silk fibroin [86], graphene oxide, mesoporous bioactive glass [87], and amnion [88]. Surface treatment of a scaffold has been tested to improve biocompatibility through hydrophilic changes [81] or to improve stability and bone forming ability using hydroxyapatite granules [89,90].…”