A novel method for automatic pharmacological evaluation of sucrose preference change in depression mice

Yin, Chunyu; Li, Liandi; Chu, Xu; Du, Ziwei; Wu, Jia-Min; Chen, Xiang; Xia, Tian; Huang, Shuying; Meng, Fanyong; Zhang, Jing; Xu, Pei-Jin; Hua, Fuzhou; Naveed, Muhammad; Han, Feng; Zhou, Qi‐Gang

doi:10.1016/j.phrs.2021.105601

Cited by 20 publications

(11 citation statements)

References 45 publications

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“…Neither GH nor CORT unmixed males displayed a sucrose preference, perhaps due to the short duration of the test (4 h). Rodents tend to drink more liquids at night during the active phase [27], and thus, more robust sucrose preferences can be obtained when animals are given access to sucrose for longer periods of time, particularly at night [28]. Finally, the chronic administration of corticosterone tended to increase total liquid consumption in all groups (Figure 3B), though these trends were not statistically significant compared to the GH controls.…”

Section: Mixed Treatment Group Housing Has No Impact On Sucrose Prefe...mentioning

confidence: 92%

Sex-Specific Social Effects on Depression-Related Behavioral Phenotypes in Mice

Patel

Cameron

Olson

2021

Life

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Social interaction and empathy play critical roles in determining the emotional well-being of humans. Stress-related depression and anxiety can be exacerbated or mitigated depending on specific social conditions. Although rodents are well known to exhibit emotional contagion and consolation behavior, the effects of group housing on stress-induced phenotypes in both males and females are not well established. Here, we investigated how the presence of stressed or unstressed conspecifics within a cage impact depression-related phenotypes. We housed male and female C57BL/6J mice in same-sex groups and subjected them to either gentle handling (GH) or the daily administration of corticosterone (CORT) for 10 days. The GH and CORT treatment groups were divided into cages of unmixed (GH or CORT) and mixed (GH and CORT) treatments. Depression-related phenotypes were measured using the forced swim test (FST) and sucrose preference test (SPT). We found that mixed housing alters FST behavior in a sex-specific manner. Male mice given chronic corticosterone (CORT) that were housed in the same cage as gently handled animals (GH) exhibited increased immobility, whereas GH females housed with CORT females demonstrated the opposite effect. This study underscores the importance of social housing conditions when evaluating stress-induced behavioral phenotypes and suggests that mixed cages of GH and CORT animals yield the greatest difference between treatment groups. The latter finding has important implications for identifying therapeutics capable of rescuing stress-induced behavioral deficits in the FST.

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Section: Mixed Treatment Group Housing Has No Impact On Sucrose Prefe...mentioning

confidence: 92%

Sex-Specific Social Effects on Depression-Related Behavioral Phenotypes in Mice

Patel

Cameron

Olson

2021

Life

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“…The experimental procedures were as described in the previous studies 18,21 . All mice were undergoing the adaptation period of sugar water and the deprivation period, respectively.…”

Section: Methodsmentioning

confidence: 99%

Differentially expressed genes of esophageal tissue in male acute and chronic sleep deprivation mice

Li,

Lu,

Yang

et al. 2024

Genes Brain and Behavior

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Gastroesophageal reflux disease (GERD) is associated with sleep disturbances. However, mechanisms underlying these interactions remain unclear. Male acute and chronic sleep deprivation (SD) mice were used for this study. Mice in the chronic SD group exhibited anxiety‐ and depression‐like behaviors. We further performed high‐throughput genome sequencing and bioinformatics analysis to screen for featured differentially expressed genes (DEGs) in the esophageal tissue. The acute SD group, comprised 25 DEGs including 14 downregulated and 11 upregulated genes. Compared with the acute SD group, more DEGs were present in the chronic SD group, with a total of 169 DEGs, including 88 downregulated and 81 upregulated genes. Some DEGs that were closely related to GERD and associated esophageal diseases were significantly different in the chronic SD group. Quantitative real‐time polymerase chain reaction verified the downregulation of Krt4, Krt13, Krt15 and Calml3 and upregulation of Baxl1 and Per3. Notably, these DEGs are involved in biological processes, which might be the pathways of the neuroregulatory mechanisms of DEGs expression.

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“…To observe the anhedonia‐like behaviour, the SPT was performed as described 21 . The process is as follows: (1) 1% sucrose solution and (2) tap water.…”

Section: Methodsmentioning

confidence: 99%

Engeletin alleviates depressive‐like behaviours by modulating microglial polarization via the LCN2/CXCL10 signalling pathway

Zhang,

Song,

Huo

et al. 2024

J Cellular Molecular Medi

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Microglial polarization and associated inflammatory activity are the key mediators of depression pathogenesis. The natural Smilax glabra rhizomilax derivative engeletin has been reported to exhibit robust anti‐inflammatory activity, but no studies to date have examined the mechanisms through which it can treat depressive symptoms. We showed that treatment for 21 days with engeletin significantly alleviated depressive‐like behaviours in chronic stress social defeat stress (CSDS) model mice. T1‐weighted imaging (T1WI), T2‐weighted imaging (T2WI) imaging revealed no significant differences between groups, but the bilateral prefrontal cortex of CSDS mice exhibited significant increases in apparent diffusion coefficient and T2 values relative to normal control mice, with a corresponding reduction in fractional anisotropy, while engeletin reversed all of these changes. CSDS resulted in higher levels of IL‐1β, IL‐6, and TNF‐a production, enhanced microglial activation, and greater M1 polarization with a concomitant decrease in M2 polarization in the mPFC, whereas engeletin treatment effectively abrogated these CSDS‐related pathological changes. Engeletin was further found to suppress the LCN2/C‐X‐C motif chemokine ligand 10 (CXCL10) signalling axis such that adeno‐associated virus‐induced LCN2 overexpression ablated the antidepressant effects of engeletin and reversed its beneficial effects on the M1/M2 polarization of microglia. In conclusion, engeletin can alleviate CSDS‐induced depressive‐like behaviours by regulating the LCN2/CXCL10 pathway and thereby altering the polarization of microglia. These data suggest that the antidepressant effects of engeletin are correlated with the polarization of microglia, highlighting a potential avenue for future design of antidepressant strategies that specifically target the microglia.

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A novel method for automatic pharmacological evaluation of sucrose preference change in depression mice

Cited by 20 publications

References 45 publications

Sex-Specific Social Effects on Depression-Related Behavioral Phenotypes in Mice

Sex-Specific Social Effects on Depression-Related Behavioral Phenotypes in Mice

Differentially expressed genes of esophageal tissue in male acute and chronic sleep deprivation mice

Engeletin alleviates depressive‐like behaviours by modulating microglial polarization via the LCN2/CXCL10 signalling pathway

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