A novel method for depositing erythroid cells onto glass slides for fetal cell analysis

Collarini, Ellen J.; Nagy, Dea; Cain, Carol A.; Gammon, Dawn; Schueler, Paula A.; Mahoney, Walt

doi:10.1002/1097-0320(20011201)45:4<304::aid-cyto10022>3.0.co;2-#

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…20 HRPconjugated canine heavy-chain (HC)-and light-chain (LC)-specific antisense probes (2 of each) were designed: HRP-cFVIII-HC probe cocktail (probe 1: 5Ј TTT TCT GGT GCC ACT AAG GCT GAA GGG CAA3Ј; probe 2: 5Ј GAG CTG TCG TCA TCA AAG CTA ACT ACG TCC 3Ј); and HRP-cFVIII-LC probe cocktail (probe 3: 5Ј GAG GCC CTG ATT TTC ATA GTC GCC GTA GAT 3Ј; probe 4: 5Ј ATT GCA GGG AGC TCT ACA GTT CCT TTC CAG 3Ј; Epoch Biosciences, Bothell, WA).…”

Section: Fish Analysismentioning

confidence: 99%

Sustained phenotypic correction of canine hemophilia A using an adeno-associated viral vector

Scallan

Lillicrap

Jiang

et al. 2003

Blood

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Section: Fish Analysismentioning

confidence: 99%

Sustained phenotypic correction of canine hemophilia A using an adeno-associated viral vector

Scallan

Lillicrap

Jiang

et al. 2003

Blood

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“…28 In brief, liver tissue was harvested and mounted in Optimal Cutting Temperature Compound (Sakura Finetek USA, Torrance, CA). Sections (12 m) were generated using a cryostat and fixed in 4% formaldehyde/PBS.…”

Section: Rna Fluorescence In Situ Hybridization (Fish) Analysis Of Camentioning

confidence: 99%

Phenotypic correction of a mouse model of hemophilia A using AAV2 vectors encoding the heavy and light chains of FVIII

Scallan

Liu

Parker

et al. 2003

Blood

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“…The isolation of rare cells from maternal blood for noninvasive prenatal diagnosis is a new field of research exhibiting several difficulties since this strategy requires several basic technological protocols for a successful outcome (19,45,46,48,50,52,62,68,(104)(105)(106)(107)(108)(109)(110)(111)(112).…”

Section: Discussionmentioning

confidence: 99%

New trends in non-invasive prenatal diagnosis: Applications of dielectrophoresis-based Lab-on-a-chip platforms to the identification and manipulation of rare cells (Review)

Borgatti

Bianchi²,

Mancini³

et al. 2008

Int J Mol Med

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Abstract. The isolation of rare cells, such as fetal nucleated red blood cells and trophoblasts, from maternal blood for noninvasive prenatal diagnosis is a new field of research exhibiting several difficulties since this strategy requires unresolved basic technological protocols for a successful outcome. However, several achievements in the field of Laboratory-on-a-chip (Labon-a-chip) technology have provided clear advancements in projects aimed at the isolation of rare cells from biological fluids. Among the most interesting approaches are those based on dielectrophoresis (DEP). DEP-based Lab-on-a-chip platforms have been demonstrated to be suitable for several applications in biotechnology and biomedicine. DEP-based arrays are able to manipulate single cells, which can be identified and moved throughout the DEP chip to recovery places. DEP buffers are compatible with molecular interactions between monoclonal antibodies and target cells, allowing integration of these devices with magnetic cell sorting (MACS). DEP treatment does not alter the viability of manipulated cells.

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A novel method for depositing erythroid cells onto glass slides for fetal cell analysis

Cited by 4 publications

References 23 publications

Sustained phenotypic correction of canine hemophilia A using an adeno-associated viral vector

Sustained phenotypic correction of canine hemophilia A using an adeno-associated viral vector

Phenotypic correction of a mouse model of hemophilia A using AAV2 vectors encoding the heavy and light chains of FVIII

New trends in non-invasive prenatal diagnosis: Applications of dielectrophoresis-based Lab-on-a-chip platforms to the identification and manipulation of rare cells (Review)

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