A novel method for dissolution and stabilization of non‐mulberry silk gland protein fibroin using anionic surfactant sodium dodecyl sulfate

Mandal, Biman B.; Kundu, Subhas C.

doi:10.1002/bit.21699

Cited by 114 publications

(98 citation statements)

References 23 publications

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“…The use of anionic surfactant, sodium dodecyl sulfate (SDS) for dissolution and regeneration of silk fibroin protein from gland of silkworm larvae has already been reported (Fig. 2) (Mandal and Kundu, 2008). Silk fibroin protein being hydrophobic in nature is water insoluble.…”

Section: Dissolution Of Silk Gland Fibroin Proteinmentioning

confidence: 98%

“…One percentage (w/v) aqueous solution of SDS containing 10 mM Tris (pH 8.0) and 5 mM EDTA was used as the fibroin dissolution buffer (Mandal and Kundu, 2008). In brief, the silk gland protein was dissolved in SDS buffer under constant stirring for 30 min at room temperature followed by a brief centrifugation at 5,000 rpm for 5 min.…”

Section: Dissolution and Regenerated Silk Fibroin Solution Preparationmentioning

confidence: 99%

“…One can extract fibroin protein in the range of 1-1.5 g per matured larva with this isolation method. This method of isolation and protein dissolution is expected to meet the growing demand for a natural polymer for tissue engineering applications (Mandal and Kundu, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Mandal

Kundu

2008

Biotech & Bioengineering

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The possibility of using wild non-mulberry silk protein as a biopolymer remains unexplored compared to domesticated mulberry silk protein. One of the main reasons for this was for not having any suitable method of extraction of silk protein fibroin from cocoons and silk glands. In this study non-bioengineered non-mulberry silk gland fibroin protein from tropical tasar silkworm Antheraea mylitta, is regenerated and characterized using 1% (w/v) sodium dodecyl sulfate (SDS). The new technique is important and unique because it uses a mild surfactant for fibroin dissolution and is advantageous over other previous reported techniques using chaotropic salts. Fabricated fibroin films are smooth as confirmed by atomic force microscopy. Circular dichroism spectrometry along with Fourier transformed infrared spectroscopy and X-ray diffraction reveal random coil/alpha-helix conformations in regenerated fibroin which transform to beta-sheets, resulting in crystalline structure and protein insolubility through ethanol treatment. Differential scanning calorimetry shows an increase in glass transition (Tg) temperature and enhanced degradation temperature on alcohol treatment. Enhanced cell attachment and viability of AH927 feline fibroblasts were observed on fibroin matrices. Higher mechanical strength along with controllable water stability of regenerated gland fibroin films make non-mulberry Indian tropical tasar silk gland fibroin protein a promising biomaterial for tissue engineering applications.

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Section: Dissolution Of Silk Gland Fibroin Proteinmentioning

confidence: 98%

Section: Dissolution and Regenerated Silk Fibroin Solution Preparationmentioning

confidence: 99%

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Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Mandal

Kundu

2008

Biotech & Bioengineering

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“…11 In addition, TSF has been extracted directly from the glands of silkworms. [15][16][17] The as-cast BSF and TSF lms are water soluble due to their random coil structure, post-treatment using organic solvents is required, such as methanol, ethanol. 18,19 To develop highly biocompatible silk broin material, using less toxic solvents is an important direction.…”

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confidence: 99%

A novel method to prepare tussah/Bombyx mori silk fibroin-based films

Yang

Wang

et al. 2018

RSC Adv.

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The possibility of using silk fibroin in biomaterials for tissue engineering is a subject of broad interest. In this study, Bombyx mori/tussah silk fibroin (BSF/TSF) blend films were prepared by solution casting using CaCl 2 / formic acid as a co-solvent and water as a rinse solvent. The morphology, crystallinity, thermal resistance, mechanical properties and water contact angle of the blend films as well as the biocompatibility were investigated. The BSF/TSF blend films displayed a smooth surface and specific nanostructure in their cross-section, originating from the nanofibril-preservation during fibroin dissolution. The water rinse process induced the formation of a stable b-sheet structure. The BSF film showed superior mechanical properties to the TSF film, and the blending with TSF led to a significant reduction in the strength and elasticity of blend films. However, adding the TSF component could regulate the hydrophilic properties and enhance cell growth on the blend films. The BSF/TSF blend films with specific nanostructure, stable secondary structure, appropriate mechanical properties, and good biocompatibility, are promising candidates for application in regenerative medicine.

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“…Despite the high microbial keratitis risk for a prolonged wear time, which subsists for most of ophthalmic applications in this field, extended wear time of silicone hydrogel lenses are five times safer than other conventional hydrogels for what concern infections [170,171]. Only about 5% of drugs administrated by eye drops are bioavailable, and currently eye drops account for more than 90% of all ophthalmic formulations.…”

Section: Hydrogels As Soft Contact Lensesmentioning

confidence: 99%

History and Applications of Hydrogels

Yahia¹

2015

J Biomed Sci

162

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Hydrogels have existed for more than half century, providing one of the earliest records of crosslinked hydroxyethyl methacrylate (HEMA) hydrogels. Today, hydrogels still fascinate material scientists and biomedical researchers and great strides have been made in terms of their formulations and applications. As a class of material, hydrogels are unique, they consist of a self-supporting, waterswollen three-dimensional (3D) viscoelastic network which permits the diffusion and attachment of molecules and cells. However, hydrogels have recently drawn great attention for use in a wide variety of biomedical applications such as cell therapeutics, wound healing, cartilage/bone regeneration and the sustained release of drugs. This is due to their biocompatibility and the similarity of their physical properties to natural tissue. This review aims to give an overview of the historic and the recent design concept of hydrogels and their several applications based on the old and the most recent publications in this field. Journal of Biomedical Sciences ISSN 2254-609XThis Article is Available in: www.jbiomeds.com 2 Hydrogels can be classified into two distinct categories, the natural and the synthetic hydrogels. Natural hydrogels include collagen, fibrin, hyaluronic acid, matrigel, and derivatives of natural materials such as chitosan, alginate and skill fibers. They remain the most physiological hydrogels as they are components of the extracellular matrix (ECM) in vivo. Two main drawbacks of natural hydrogels, however, make their final microstructures and properties difficult to control reproducibly between experiments. First, the fine details of their mechanical properties and their dependence on polymerization or gelation conditions are often poorly understood. Second, due to their natural origin (bovine fibrinogen, rat tail collagen… their composition may vary from one batch to another.In contrast, synthetic hydrogels such as poly (ethylene glycol) diacrylate, poly(acryl amide), poly(vinyl alcohol) are more reproducible, although their final structure can also depend on polymerization conditions in a subtle way, so that a rigorous control of the preparation protocol, including temperature and environment control, may be necessary. Generally speaking, synthetic hydrogels offer more flexibility for tuning chemical composition and mechanical properties; users can, for example vary the concentration or molecular weight of the precursor, or alter the percentage of crosslinkers. They can also be selected or tuned to be hydrolysable or biodegradable over variable periods of time.Hydrogels may be chemically stable or they may degrade and eventually disintegrate and dissolve. They are called «reversible» or «physical» gels when the networks are held together by molecular entanglements, and/or secondary forces including ionic, H-bonding or hydrophobic forces (Figure 2) [3]. Physical hydrogels are not homogeneous, since clusters of molecular entanglements, or hydrophobically-or ionically-associated domains, can create in h...

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A novel method for dissolution and stabilization of non‐mulberry silk gland protein fibroin using anionic surfactant sodium dodecyl sulfate

Cited by 114 publications

References 23 publications

Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

A novel method to prepare tussah/Bombyx mori silk fibroin-based films

History and Applications of Hydrogels

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