A Novel Method for Effective Cell Segmentation and Tracking in Phase Contrast Microscopic Images

Abstract

Cell migration plays an important role in the identification of various diseases and physiological phenomena in living organisms, such as cancer metastasis, nerve development, immune function, wound healing, and embryo formulation and development. The study of cell migration with a real-time microscope generally takes several hours and involves analysis of the movement characteristics by tracking the positions of cells at each time interval in the images of the observed cells. Morphological analysis considers the shapes of the cells, and a phase contrast microscope is used to observe the shape clearly. Therefore, we developed a segmentation and tracking method to perform a kinetic analysis by considering the morphological transformation of cells. The main features of the algorithm are noise reduction using a block-matching 3D filtering method, k-means clustering to mitigate the halo signal that interferes with cell segmentation, and the detection of cell boundaries via active contours, which is an excellent way to detect boundaries. The reliability of the algorithm developed in this study was verified using a comparison with the manual tracking results. In addition, the segmentation results were compared to our method with unsupervised state-of-the-art methods to verify the proposed segmentation process. As a result of the study, the proposed method had a lower error of less than 40% compared to the conventional active contour method.

Topography of pleural epithelial structure enabled by en face isolation and machine learning

“…Single‐cell segmentation of epithelium, the process that identifies and outlines individual cells in an image, is one of the most difficult tasks in biological image analysis (Akram et al, 2016; Van Valen et al, 2016). Conventional image segmentation uses watershed algorithms (Jo et al, 2021; Kandel et al, 2021; Wang et al, 2019). Watershed algorithms identify gray‐level inflection as a boundary even if the change is subtle.…”