A Novel Method for Evaluating the Acrosomal Status of Mammalian Spermatozoa

Margalit, I; Rubinstein, Sara; Breitbart, Haim

doi:10.3109/01485019708987906

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…The most widely utilized are those that involve the use of lectins or dyes. In the first case, the most widely used are Pisum sativum agglutinin (PSA; Margalit et al, 1997), concanavalin A (Holden et al, 1990) or Peanut agglutinin (PNA) (Mortimer et al, 1987). Among the different dyes, Coomassie blue staining (Larson & Miller, 1999) has been extensively used in mouse sperm.…”

Section: Challenges To Studying Armentioning

confidence: 99%

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Balestrini

Jabloñski

Schiavi‐Ehrenhaus

et al. 2020

Molecular Reproduction Devel

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Acrosomal exocytosis (AR) is a critical process that sperm need to undergo to fertilize an egg. The evaluation of the presence or absence of the acrosome is usually performed by using lectins or dyes in fixed cells. With this approach, it is neither possible to monitor the dynamic process of exocytosis and related molecular events while discriminating between live and dead cells, nor to evaluate the acrosomal status while sperm reside in the female reproductive tract. However, over the last two decades, several new methodologies have been used to assess the occurrence of AR in living cells allowing different groups to obtain information that was not possible in the past. These techniques have revolutionized the whole study of this process. This review summarizes current methods available to analyze AR in living cells as well as the important information that emerged from studies using these approaches.

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Section: Challenges To Studying Armentioning

confidence: 99%

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Balestrini

Jabloñski

Schiavi‐Ehrenhaus

et al. 2020

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…The proportion of acrosome-intact and acrosomereacted (AR) spermatozoa was assessed after cell labeling with FITC-PSA [44]; parallel evaluations with Nomarski differential interference contrast microscopy were done, validating its use for the assessment of acrosomal integrity, as previously reported [45]. Aliquots of sperm cells recovered after each treatment were fixed with 2% (vol/vol) formaldehyde in Dulbecco-PBS (D-PBS), washed, smeared onto slides and air dried.…”

Section: Assessment Of Sperm Acrosomal Statusmentioning

confidence: 70%

Epithelial cadherin is present in bovine oviduct epithelial cells and gametes, and is involved in fertilization-related events

et al. 2014

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Delivering value from sperm proteomics for fertility

et al. 2012

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Fertilization of an egg by a spermatozoon sets the stage for mammalian development. Viable sperm are a prerequisite for successful fertilization and beyond. Spermatozoa have a unique cell structure where haploid genomic DNA is located in a tiny cytoplasmic space in the head, mitochondria in the midpiece and then the tail, all enclosed by several layers of membrane. Proteins in sperm play vital roles in motility, capacitation, fertilization, egg activation and embryo development. Molecular defects in these proteins are associated with low fertility or in some cases, infertility. This review will first summarize genesis, molecular anatomy and physiology of spermatozoa, fertilization, embryogenesis and then those proteins playing important roles in various aspects of sperm physiology.

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A Novel Method for Evaluating the Acrosomal Status of Mammalian Spermatozoa

Cited by 5 publications

References 42 publications

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

Epithelial cadherin is present in bovine oviduct epithelial cells and gametes, and is involved in fertilization-related events

Delivering value from sperm proteomics for fertility

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