A novel method for immobilization of proteins via entrapment of magnetic nanoparticles through epoxy cross-linking

Iype, Tessy; Thomas, Jaiby; Mohan, Sangeetha; Johnson, Kochurani K.; George, Ligi E.; Ambattu, Lizebona August; Bhati, Aniruddha; Ailsworth, Kristen; Menon, Bindu; Rayabandla, Sunayana M.; Jesudasan, Rachel A.; Santhosh, Sam; Ramchand, C.N.

doi:10.1016/j.ab.2016.12.007

Cited by 32 publications

(22 citation statements)

References 25 publications

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“…One novel approach to enzyme immobilization is based on the entrapment of magnetic nanoparticles via epoxy cross-linking. This technique can be employed to improve the activity of pollutant degradation bacteria, fungi, or heterologous-expressed enzymes (Iype et al, 2017;Gangola et al, 2018). Recent high throughput techniques can be used to explore key functional strains from the pesticide degradation microbial community and fulfill the gap between laboratory cultures and large-scale applications .…”

Section: Novel Technologiesmentioning

confidence: 99%

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

et al. 2020

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Lindane (γ-hexachlorocyclohexane) is an organochlorine pesticide that has been widely used in agriculture over the last seven decades. The increasing residues of lindane in soil and water environments are toxic to humans and other organisms. Large-scale applications and residual toxicity in the environment require urgent lindane removal. Microbes, particularly Gram-negative bacteria, can transform lindane into non-toxic and environmentally safe metabolites. Aerobic and anaerobic microorganisms follow different metabolic pathways to degrade lindane. A variety of enzymes participate in lindane degradation pathways, including dehydrochlorinase (LinA), dehalogenase (LinB), dehydrogenase (LinC), and reductive dechlorinase (LinD). However, a limited number of reviews have been published regarding the biodegradation and bioremediation of lindane. This review summarizes the current knowledge regarding lindane-degrading microbes along with biodegradation mechanisms, metabolic pathways, and the microbial remediation of lindane-contaminated environments. The prospects of novel bioremediation technologies to provide insight between laboratory cultures and largescale applications are also discussed. This review provides a theoretical foundation and practical basis to use lindane-degrading microorganisms for bioremediation.

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Section: Novel Technologiesmentioning

confidence: 99%

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

et al. 2020

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“…Amongst the coupling agents, ECH is generally preferred because it can be readily removed from the resultant materials and shows high yield even at low amounts of use and demonstrates good biocompatibility of the end‐products . Furthermore, ECH is commonly utilized as an coupling agent for removal of bacterial endotoxins, in waste water treatment, drug delivery systems, enzyme immobilization, food packaging, wound healing and many other applications . Once ECH anchorage is accomplished on the surface, then the epoxide surface can readily react with amine groups in basic media such as PEI .…”

Section: Introductionmentioning

confidence: 99%

Functionalization of halloysite nanotubes with polyethyleneimine and various ionic liquid forms with antimicrobial activity

Suner

Şahiner

Akçalı

et al. 2019

J of Applied Polymer Sci

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Halloysite nanotube (HNT), a natural clay, was modified with branched polyethyleneimine (PEI) to form PEI‐HNT using epichlorohydrin (ECH) as coupling agent, then protonated with HCl to obtain H‐PEI‐HNTs providing [NH3]+[Cl]− functionality for potential antimicrobial properties. Upon PEI modification, zeta potential value of HNTs was increased to +37.3 mV from −34.5 mV and to +41.1 mV for H‐PEI‐HNTs. Only 1.87 wt % H‐element in HNT was increased to 3.03 wt % upon PEI modification along with newly generated elements of N and C at 2.99 and 9.93 wt %, respectively. Moreover, ionic liquid (IL) forms of HNTs with [NH3]+[N(CN)2]−, [NH3]+[PF6]− and [NH3]+[BF4]− functionality were generated via anion exchange of H‐PEI‐HNTs with sodium dicyanamide (SDC), ammonium hexafluorophosphate (AHFP), and sodium tetrafluoroborate (STFB). The antimicrobial properties of the modified, protonated, and IL forms of HNTs were determined via macro dilution, diffusion and agar screening tests against Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 10145, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538 strains, and Candida albicans ATCC 10231 strains. It was found that H‐PEI‐HNTs possesses potent antimicrobial effect compared with the other forms of HNTs with 2–4 mg mL−1 MIC and 8–16 mg mL−1 MBC values via the macro dilution method. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48352.

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“…It was suggested that the chemical crosslinking is an effective approach to significantly build up the performance of SPI-based films. From the literature review, the most common used crosslinking agents were aldehydes (e.g., glutaraldehyde, glyoxal, and formaldehyde) [31,32], phenolic derivatives [27], and epoxy compounds [25,33,34,35].…”

Section: Introductionmentioning

confidence: 99%

Sodium Hydroxide-Free Soy Protein Isolate-Based Films Crosslinked by Pentaerythritol Glycidyl Ether

Cai

Wang

et al. 2018

Polymers

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The soy protein isolate (SPI), sodium dodecylbenzenesulfonate (SDBS) and pentaerythritol glycidyl ether (PEGE) were used to make biodegradable films in this study. Unlike the usual method that adding sodium hydroxide (NaOH) during the SPI-based film casting, SDBS was used as a surfactant playing the similar role as NaOH. Since NaOH is a chemical with corrosiveness and toxicity, the replacing of NaOH by SDBS might reduce the hazard threat during the utilization of SPI-based films in food packing application. Furthermore, the presentation of SDBS helped dispersing the hydrophobic PEGE into the hydrophilic SPI. PEGE is a crosslinking agent with multiple reactive epoxy groups. The chemical structures and micro morphologies of the fabricated films were investigated by means of FTIR, XRD, and SEM. The thermal stabilities of the films were examined by means of the thermo-gravimetric analysis. After the chemical crosslinking, the ultimate tensile strength of the film was significantly increased, meanwhile, the water absorption was dramatically decreased. It was concluded that the SPI-based film containing 4% PEGE achieved the optimal performance.

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A novel method for immobilization of proteins via entrapment of magnetic nanoparticles through epoxy cross-linking

Cited by 32 publications

References 25 publications

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

Functionalization of halloysite nanotubes with polyethyleneimine and various ionic liquid forms with antimicrobial activity

Sodium Hydroxide-Free Soy Protein Isolate-Based Films Crosslinked by Pentaerythritol Glycidyl Ether

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