A novel method for measuring small amounts of saliva in mice

Arakaki, Rieko; Ushio, Aya; Otsuka, Kunihiro; Kudo, Yasusei; Ishimaru, Naozumi

doi:10.1002/osi2.1013

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…[ 54 ] Pec‐PG hydrogel exhibited excellent mucoadhesion on the porcine tongue (Figure 2b,c), indicating great potential as a mucoadhesive hydrogel in humans. Although sprayable Pec‐PG could be readily crosslinked in rodents due to higher pH levels (≈pH 9) in rodent oral cavity compared to humans (≈pH 6.7 to 7.3), [ 55 ] we may need to test crosslinking and hydrogel adhesion of sprayable Pec‐PG in larger animals with similar pH level to the human oral cavity. In summary, human physiological conditions should be considered for the practicality and clinical impact of our mucoadhesive pectin hydrogels for oral applications.…”

Section: Resultsmentioning

confidence: 99%

Mucoadhesive Phenolic Pectin Hydrogels for Saliva Substitute and Oral Patch

Choi

Jeon

Bae

et al. 2023

Adv Funct Materials

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Oral disease is one of the most common conditions worldwide, negatively affecting general health, reducing the quality of life, and often developing into systemic illness. However, the design of therapeutic agents for oral diseases is challenging due to various unique features of the oral cavity, including its wet and dynamic environment and curved shape. Herein, the development of highly biocompatible mucoadhesive functional hydrogels for oral applications is reported, generated by introducing bio‐inspired phenolic moieties into a pectin polymer. Pyrogallol‐functionalized pectin (Pec‐PG) can be crosslinked in situ via autoxidation without chemical agents and readily fabricated as various formulations. Sprayable Pec‐PG hydrogel exhibits strong mucoadhesion and outstanding hydration ability ex vivo and in vivo, thus displaying significant potential as a novel saliva substitute for dry mouth. The authors further show that topical application of mucoadhesive Pec‐PG patches pre‐loaded with corticosteroid significantly promotes the repair of diabetic oral ulcer tissue via prolonged drug release, free radical scavenging, and physical barrier effects. Moreover, similar applications for oral ulcer treatment using a pectin hydrogel modified with catechol (Pec‐CA), another phenolic moiety are demonstrated. Together, these findings suggest that mucoadhesive phenolic pectin derivatives can provide highly biocompatible, convenient, and effective hydrogel platforms for treating oral diseases.

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Section: Resultsmentioning

confidence: 99%

Mucoadhesive Phenolic Pectin Hydrogels for Saliva Substitute and Oral Patch

Choi

Jeon

Bae

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Another drawback is that the protein concentration of the target biomarker might be considerably lower compared to plasma concentrations, which might make it technically difficult to quantify accurately and robustly [ 1 ]. Additionally, collecting saliva from research animals such as rodents is possible but more technically difficult than blood, and might represent a challenge from a translational medicine perspective [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Saliva and Saliva Extracellular Vesicles for Biomarker Candidate Identification—Assay Development and Pilot Study in Amyotrophic Lateral Sclerosis

Sjoqvist

Otake

2023

IJMS

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Saliva is gaining increasing attention as a source of biomarkers due to non-invasive and undemanding collection access. Extracellular vesicles (EVs) are nano-sized, cell-released particles that contain molecular information about their parent cells. In this study, we developed methods for saliva biomarker candidate identification using EV-isolation and proteomic evaluation. We used pooled saliva samples for assay development. EVs were isolated using membrane affinity-based methods followed by their characterization using nanoparticle tracking analysis and transmission electron microscopy. Subsequently, both saliva and saliva-EVs were successfully analyzed using proximity extension assay and label-free quantitative proteomics. Saliva-EVs had a higher purity than plasma-EVs, based on the expression of EV-proteins and albumin. The developed methods could be used for the analysis of individual saliva samples from amyotrophic lateral sclerosis (ALS) patients and controls (n = 10 each). The starting volume ranged from 2.1 to 4.9 mL and the amount of total isolated EV-proteins ranged from 5.1 to 42.6 µg. Although no proteins were significantly differentially expressed between the two groups, there was a trend for a downregulation of ZNF428 in ALS-saliva-EVs and an upregulation of IGLL1 in ALS saliva. In conclusion, we have developed a robust workflow for saliva and saliva-EV analysis and demonstrated its technical feasibility for biomarker discovery.

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A novel method for measuring small amounts of saliva in mice

Cited by 2 publications

References 5 publications

Mucoadhesive Phenolic Pectin Hydrogels for Saliva Substitute and Oral Patch

Mucoadhesive Phenolic Pectin Hydrogels for Saliva Substitute and Oral Patch

Saliva and Saliva Extracellular Vesicles for Biomarker Candidate Identification—Assay Development and Pilot Study in Amyotrophic Lateral Sclerosis

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