A novel method for preparing liposome with a high capacity to encapsulate proteinous drugs: Freeze-drying method.

Ohsawa, Takashi; Miura, Hiroshi; Harada, Kiyoshi

doi:10.1248/cpb.32.2442

Cited by 28 publications

(6 citation statements)

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“…Kirby and Gregoriadis (1984) reported that dehydration-rehydration of either SUV or MLV made of various lipid compositions produces liposomes of high encapsulation efficiency. Similar results were obtained by Ohsawa et al (1984) using PC SUV. In both cases the resultant liposomes were superior, in terms of their encapsulation efficiency, to MLV prepared by the dry-film method.…”

Section: A Spontaneous Furion Of Smar Unilamellar Veskks In the Gel supporting

confidence: 87%

Liposomes: Preparation, Characterization, and Preservation

Lichtenberg

Barenholz

1988

Methods of Biochemical Analysis

244

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Section: A Spontaneous Furion Of Smar Unilamellar Veskks In the Gel supporting

confidence: 87%

Liposomes: Preparation, Characterization, and Preservation

Lichtenberg

Barenholz

1988

Methods of Biochemical Analysis

244

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“…This phenomenon was previously reported to enhance the drug loading in niosomes, 34 but no electronic imaging evidence were provided to support this niosomal fusion. The same mechanism was also reported by Ohsawa et al, 42 using the well-known freeze-drying method to enhance the encapsulation of biomolecules and drugs in liposomes. The mechanism explained to promote the encapsulation during the freeze thawing cycle is based on: 34,39 (1) the drug and vesicles are concentrated while freezing, (2) vesicles were closely packed with each other resulting in their fusion, (3) new vesicles are formed, enhancing the efficient entrapment of drugs or active biomolecules.…”

Section: Synthesis Of Niosome By Batch and Continuoussupporting

confidence: 75%

Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors

García-Salinas

Himawan

Mendoza

et al. 2018

ACS Appl. Mater. Interfaces

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The large-scale continuous production of niosomes remains challenging. The inherent drawbacks of batch processes such as large particle polydispersity and reduced batch-to-batch reproducibility are here overcome by using commercially available microfluidic reactors. Compared to the traditional batch-based film hydration method, herein, we demonstrate that it is possible to carry out the homogeneous, large-scale (up to 120 mg/min) production of niosomes using two different synthesis techniques (the thin film hydration method and the emulsification technique). Niosomes particle size can be controlled depending on the need by varying the synthesis temperature. The high cytocompatibility of the resulting niosomes was also demonstrated in this work on three different somatic cell lines. For the first time, the structure of the niosome multilamellar shell was also elucidated using high-resolution transmission electron microscopy (HR-STEM) as well as their colloidal stability over time (6 weeks) under different storage conditions. The morphology of cryo-protected or as-made niosomes was also evaluated by HR-STEM after freeze-drying. Finally, the dual ability of those synthetic, nonionic, surfactant-based vesicles to carry both hydrophilic and hydrophobic molecules was also here demonstrated by using laser scanning confocal microscopy.

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“…Another method of preparation previously described involves the mixing of a preformed SUVs dispersion with an aqueous solution of the drug to be encapsulated, followed by lyophilization and rehydration of the mixture (Kirby and Gregoriadis, 1984;Ohsawa et al, 1984). This technique also allows for the formation of MLVs that achieve an encapsulation efficiency of up to 40% (Kirby and Gregoriadis, 1984;Ohsawa et al, 1984).…”

Section: B Types Of Liposomesmentioning

confidence: 99%