A Novel Method for Preserving Human Lungs Using a Super-Cooling System

Abe, Masayoshi; Jimi, Shiro; Hama, Hiroshi; Shiraishi, Takeshi; Iwasaki, Atsushi; Ono, Nobuhumi; Shirakusa, Takayuki; Katsuragi, Takeshi

doi:10.1016/j.athoracsur.2006.03.016

Cited by 14 publications

(8 citation statements)

References 8 publications

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“…Although there have been limited data about organ preservation solutions used in the supercooling condition, such as ET-Kyoto solution, University of Wisconsin solution, 8,9 and Euro-Collins solution, 13 these solutions have a tendency to freeze in sub-zero temperatures without a supercooling refrigerator or anti-freezing proteins, 8,9 because of a restricted effect of freezing point depression. In this investigation, the sub-zero preservation was done using ET-Kyoto solution, which contains trehalose, a disaccharide known as a cryoprotectant.…”

Section: Discussionmentioning

confidence: 99%

“…A stable supercooling state has been reported to be achieved with an electric field generated by the power voltage of 3,000 volts. 13 For the preservation at 4°C, a conventional refrigerator (MPR-411F; Sanyo Electric Co, Ltd, Osaka, Japan) was used.…”

Section: Refrigeratorsmentioning

confidence: 99%

“…In addition, a decreased level of single-strand DNA has also been shown in human lungs after 5 days of storage at Ϫ5°C. 13 However, the pulmonary physiologic function was not evaluated in these previous studies, and such an evaluation is essential for the application of this new technique to clinical transplantations.…”

mentioning

confidence: 98%

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Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Okamoto

Nakamura

Zhang

et al. 2008

The Journal of Heart and Lung Transplantation

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Section: Discussionmentioning

confidence: 99%

Section: Refrigeratorsmentioning

confidence: 99%

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Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Okamoto

Nakamura

Zhang

et al. 2008

The Journal of Heart and Lung Transplantation

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“…The earliest works on supercooling preservation (SCP) date back to the 1960s with storage of bacterial and yeast cells [6,7] followed intermittently with other cell types such as, peripheral blood stem cells [8], turkey spermatozoa [9,10], cells of various rat organs [11–15]. More recent studies also include short term organ storage examples on heart [16,17], liver [16,18–21], lung [22], and kidney [16]. The temperature range that has been studied in these cell and organ studies goes from slightly below 0 o C for organs [16–23], to -5 o C for mammalian cells [8–15], and all the way down to -30 to -40 o C for bacterial and yeast cells [6,7].…”

Section: Introductionmentioning

confidence: 99%

Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes

et al. 2013

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Supercooling preservation holds the potential to drastically extend the preservation time of organs, tissues and engineered tissue products, and fragile cell types that do not lend themselves well to cryopreservation or vitrification. Here, we investigate the effects of supercooling preservation (SCP at -4oC) on primary rat hepatocytes stored in cryovials and compare its success (high viability and good functional characteristics) to that of static cold storage (CS at +4oC) and cryopreservation. We consider two prominent preservation solutions a) Hypothermosol (HTS-FRS) and b) University of Wisconsin solution (UW) and a range of preservation temperatures (-4 to -10 oC). We find that there exists an optimum temperature (-4oC) for SCP of rat hepatocytes which yields the highest viability; at this temperature HTS-FRS significantly outperforms UW solution in terms of viability and functional characteristics (secretions and enzymatic activity in suspension and plate culture). With the HTS-FRS solution we show that the cells can be stored for up to a week with high viability (~56%); moreover we also show that the preservation can be performed in large batches (50 million cells) with equal or better viability and no loss of functionality as compared to smaller batches (1.5 million cells) performed in cryovials.

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“…They extended the supercooling technology for human red blood cell preservation at − 16 °C for an extended period of 100 days and demonstrated the significantly higher recovery rates of hemoglobin. The application of high-pressure technology has been investigated for enhancing supercooling of aqueous glycerol solutions (Miyata et al, 2012 ) and human lung tissues at − 5 °C (Abe et al, 2006 ). Wan et al ( 2018 ) demonstrated that rat hearts can be preserved at subzero temperatures without ice formation by combining high-pressure treatment.…”

Section: Emerging Technologies For Extended Supercoolingmentioning

confidence: 99%

Supercooling preservation technology in food and biological samples: a review focused on electric and magnetic field applications

Kang

You

Jun

2020

Food Sci Biotechnol

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Freezing has been widely recognized as the most common process for long-term preservation of perishable foods; however, unavoidable damages associated with ice crystal formation lead to unacceptable quality losses during storage. As an alternative, supercooling preservation has a great potential to extend the shelf-life and maintain quality attributes of fresh foods without freezing damage. Investigations for the application of external electric field (EF) and magnetic field (MF) have theorized that EF and MF appear to be able to control ice nucleation by interacting with water molecules in foods and biomaterials; however, many questions remain open in terms of their roles and influences on ice nucleation with little consensus in the literature and a lack of clear understanding of the underlying mechanisms. This review is focused on understanding of ice nucleation processes and introducing the applications of EF and MF for preservation of food and biological materials.

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A Novel Method for Preserving Human Lungs Using a Super-Cooling System

Cited by 14 publications

References 8 publications

Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes

Supercooling preservation technology in food and biological samples: a review focused on electric and magnetic field applications

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