A Novel Method for Quantitative Analysis of Anti-MUC1 Expressing Ovarian Cancer Cell Surface Based on Magnetic Cell Separation

Shahbazi-G, Daryoush; Abdolahi, Mohammad

doi:10.3923/jms.2012.256.266

Cited by 9 publications

(12 citation statements)

References 17 publications

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“…These results are in good agreement with previously published results [16] which also showed a high affinity of SPIONs-C595 for OVCAR3 cell line. …”

Section: Discussionsupporting

confidence: 93%

“…The nanoprobe was synthesized using a three-step process as described in previous publications [11, 16, 17]. …”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxic effects of Nanomag-D-SPIO particles and the corresponding C595 mAb conjugated nanoparticles (SPIONs-C595) in vitro against cell lines were examined by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay which is described in a previous published study [16]. All experiments were performed in triplicate, and cell survival was determined as a percentage of viable cells in comparison with controls.…”

Section: Methodsmentioning

confidence: 99%

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Detection of MUC1‐Expressing Ovarian Cancer by C595 Monoclonal Antibody‐Conjugated SPIONs Using MR Imaging

Shahbazi‐Gahrouei

Abdolahi

2013

The Scientific World Journal

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The aim of this study is to find out the development and application of MUC1-expressing ovarian cancer (OVCAR3) by C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs) using MR imaging. At the end, its use as a nanosized contrast agent MR imaging probe for ovarian cancer detection was investigated. The strategy is to use SPIONs attached to C595 mAb that binds to the MUC1, to specifically detect ovarian cancer cells. Anticancer effects and MR imaging parameters of the prepared nanoconjugate was investigated both under in vitro and in vivo experiments. The characterization of nanoconjugate includes its size, cell toxicity, flow cytometry, Prussian blue staining test and its cellular uptake as well as its biodistribution, and MR imaging was also investigated. The findings of the study showed good tumor accumulation and detection, no in vivo toxicity, and potential selective antiovarian cancer activity. Overall, based on the findings SPIONs-C595 nanosized probe is a selective ovarian molecular imaging modality. Further subsequent clinical trials appear warranted.

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“…These results are in good agreement with previously published results [16] which also showed a high affinity of SPIONs-C595 for OVCAR3 cell line. …”

Section: Discussionsupporting

confidence: 93%

“…The nanoprobe was synthesized using a three-step process as described in previous publications [11, 16, 17]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Detection of MUC1‐Expressing Ovarian Cancer by C595 Monoclonal Antibody‐Conjugated SPIONs Using MR Imaging

Shahbazi‐Gahrouei

Abdolahi

2013

The Scientific World Journal

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“…[18] All experiments were performed in triplicate and cell survival was determined as a percentage of viable cells in comparison with controls.…”

Section: Methodsmentioning

confidence: 99%

Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection

Shahbazi‐Gahrouei¹,

Abdolahi²

2013

J Med Phys

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Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

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“…At the end of the incubation, the supernant was aspirated carefully and the water insoluble formazan salt was solubilised in 100 µl DMSO per well. After 10 min incubation at 37°C, optical density of the violet was measured by a microplate reader at a primary wavelength of 570 nm and a reference wavelength of 620 nm [22].…”

Section: Cell Viability Testmentioning

confidence: 99%

Breast cancer cell targeted MR molecular imaging probe: Anti-MUC1 antibody-based magnetic nanoparticles

Khaniabadi¹,

Majid

Asif

et al. 2017

J. Phys.: Conf. Ser.

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A Novel Method for Quantitative Analysis of Anti-MUC1 Expressing Ovarian Cancer Cell Surface Based on Magnetic Cell Separation

Cited by 9 publications

References 17 publications

Detection of MUC1‐Expressing Ovarian Cancer by C595 Monoclonal Antibody‐Conjugated SPIONs Using MR Imaging

Detection of MUC1‐Expressing Ovarian Cancer by C595 Monoclonal Antibody‐Conjugated SPIONs Using MR Imaging

Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection

Breast cancer cell targeted MR molecular imaging probe: Anti-MUC1 antibody-based magnetic nanoparticles

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