A novel method for selection of invasive tumor cells: Derivation and characterization of highly metastatic K1735 melanoma cell lines based onin vitro andin vivo invasive capacity

Kalebic, Tea; Williams, Jeannette E.; Talmadge, James E.; Kao-Shan, Chien Song; Kravitz, Betsy; Locklear, Kimberly; Siegal, Gene P.; Liotta, Lance A.; Sobel, Mark E.; Steeg, Patricia S.

doi:10.1007/bf01753577

Cited by 23 publications

(10 citation statements)

References 33 publications

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“…For invasiveness after periostin small interfering RNA (siRNA) treatment, we used 50 Ag of EHS extract in these experiments because MSCC-Inv1 cells have a high invasive activity. After incubation, we collected the penetrating cells onto the lower side of the filter to isolate highly invasive clones by the method of Kalebic et al with minor modification (12). To examine the invasiveness, cells formalin-fixed and stained with H&E. The invasiveness of the cells was determined by counting the penetrating cells onto the lower side of the filter through the pores under a microscope at Â100 magnification.…”

Section: Methodsmentioning

confidence: 99%

Periostin Promotes Invasion and Anchorage-Independent Growth in the Metastatic Process of Head and Neck Cancer

et al. 2006

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Head and neck squamous cell carcinoma (HNSCC) is one of the most common types of human cancer. Typically, HNSCC cells show persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. However, molecular mechanisms associated with the invasion and metastasis of HNSCC remain poorly understood. Here, we identified periostin as an invasion-promoting factor in HNSCC by comparing the gene expression profiles between parent HNSCC cells and a highly invasive clone. Indeed, periostin overexpression promoted invasion and anchorage-independent growth both in vitro and in vivo in HNSCC cells. Moreover, periostin-overexpressing cells spontaneously metastasized to cervical lymph nodes and to the lung through their aggressive invasiveness in an orthotopic mouse model of HNSCC. Interestingly, periostin was highly expressed in HNSCCs in comparison with normal tissues, and the level of periostin expression was well correlated with the invasiveness of HNSCC cases. In summary, these findings suggest that periostin plays an important role in the invasion and anchorage-independent growth of HNSCC. (Cancer Res 2006; 66(14): 6928-35)

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Section: Methodsmentioning

confidence: 99%

Periostin Promotes Invasion and Anchorage-Independent Growth in the Metastatic Process of Head and Neck Cancer

et al. 2006

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“…In vitro invasion assay was done as described previously (4,13). Briefly, invasion was measured by use of a 24-well cell culture insert with 8-Am pores (3097, Falcon, Becton Dickinson).…”

Section: Methodsmentioning

confidence: 99%

IFN-Induced Transmembrane Protein 1 Promotes Invasion at Early Stage of Head and Neck Cancer Progression

Hatano

Kudo

Ogawa

et al. 2008

Clinical Cancer Research

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Purpose: Head and neck squamous cell carcinoma (HNSCC) shows persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. However, molecular mechanisms associated with invasion of HNSCC remain poorly understood. We identified IFNinduced transmembrane protein 1 (IFITM1) as a candidate gene for promoting the invasion of HNSCC by comparing the gene expression profiles between parent and a highly invasive clone. Therefore, we examined the role of IFITM1in the invasion of HNSCC. Experimental Design: IFITM1 expression was examined in HNSCC cell lines and cases by reverse transcription^PCR and immunohistochemistry. IFITM1 overexpressing and knockdown cells were generated, and the invasiveness of these cells was examined by in vitro invasion assay. Gene expression profiling of HNSCC cells overexpressing IFITM1versus control cells was examined by microarray. Results: HNSCC cells expressed IFITM1mRNA at higher levels, whereas normal cells did not. By immunohistochemistry, IFITM1 expression was observed in early invasive HNSCC and invasive HNSCC. Interestingly, IFITM1 was expressed at the invasive front of early invasive HNSCC, and higher expression of IFITM1 was found in invasive HNSCC. In fact, IFITM1 overexpression promoted and IFITM1knockdown suppressed the invasion of HNSCC cells in vitro. Gene expression profiling of HNSCC cells overexpressing IFITM1 versus control cells revealed that several genes, including matrix metalloproteinase, were up-regulated in IFITM1overexpressing cells. Conclusion: Our findings suggest that IFITM1plays an important role for the invasion at the early stage of HNSCC progression and that IFITM1can be a therapeutic target for HNSCC.Head and neck squamous cell carcinoma (HNSCC) is one of the most common types of human cancer, with an annual incidence of >500,000 cases worldwide (1). The extent of lymph node metastasis is a major determinant in the prognosis of HNSCC. Like most epithelial cancers, HNSCC develops through the accumulation of multiple genetic and epigenetic alterations in a multistep process (2). Recent molecular studies have advanced our understanding of the disease and provided a rationale to develop novel strategies for early detection, classification, prevention, and treatment. Attempts to identify the genes involved in the metastasis are pivotal for the early prediction of HNSCC behavior. The process of metastasis consists of sequential and selective steps, including proliferation, induction of angiogenesis, detachment, motility, invasion into circulation, aggregation and survival in the circulation, cell arrest in distant capillary beds, and extravasation into organ parenchyma (2). We previously established an HNSCC cell line, MSCC-1, from lymph node metastasis (3). Moreover, we isolated a highly invasive clone MSCC-Inv1 from MSCC-1 cells by using an in vitro invasion assay device (4). Then, we compared the transcriptional profile of parent cells (MSCC-1) and a highly invasive clone (MSCC-Inv1) by microarray analysis to identify g...

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“…The lower compartment contained 0.5 ml of serum-free medium. After trypsinisation, 1.5 Â 10 5 cells were resuspended in 100 ml of serum-free medium and placed in the upper compartment of the cell culture insert for 18 h. After incubation, we collected the penetrating cells onto the lower side of the filter to isolate highly invasive clones by the method of Kalebic et al (1998) with minor modifications. To examine the invasiveness, cells were fixed with formalin and stained with haematoxylin.…”

Section: In Vitro Invasion Assaymentioning

confidence: 99%

Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer

et al. 2006

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Oral squamous-cell carcinoma (OSCC) is one of the most common types of human cancer. Typically OSCC cells show persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. We previously identified Periostin as the gene demonstrating the highest fold change expression in the invasive clone by comparing the transcriptional profile of parent OSCC cell line and a highly invasive clone. Here, we demonstrated that Periostin overexpression enhanced invasiveness in oral cancer cell lines. To know the role of Periostin in invasion, angiogenesis and metastasis in OSCC cases, we first examined the expression of Periostin mRNA in 31 OSCC cases by RT -PCR and Periostin protein in 74 OSCC cases by immunohistochemistry. Then, we compared the Periostin expression with invasion pattern, metastasis and blood vessel density. Periostin mRNA and protein overexpression were frequently found in OSCC cases and Periostin expression was well correlated with the invasion pattern and metastasis. Moreover, blood vessel density of Periostin-positive cases was higher than those of Periostin-negative cases. Interestingly, recombinant Periostin enhanced capillary formation in vitro in a concentration-dependant manner. In summary, these findings suggest that Periostin may promote invasion and angiogenesis in OSCC, and that Periostin can be a strong marker for prediction of metastasis in oral cancer patients.

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A novel method for selection of invasive tumor cells: Derivation and characterization of highly metastatic K1735 melanoma cell lines based onin vitro andin vivo invasive capacity

Cited by 23 publications

References 33 publications

Periostin Promotes Invasion and Anchorage-Independent Growth in the Metastatic Process of Head and Neck Cancer

Periostin Promotes Invasion and Anchorage-Independent Growth in the Metastatic Process of Head and Neck Cancer

IFN-Induced Transmembrane Protein 1 Promotes Invasion at Early Stage of Head and Neck Cancer Progression

Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer

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