A novel method for simultaneously screening superoxide anion scavengers and xanthine oxidase inhibitors using hydroethidine as a fluorescent probe coupled with high-performance liquid chromatography-mass spectrometry

Abstract

Excess reactive oxygen species can cause cellular damage, and are involved in many pathological processes such as inflammation, atherosclerosis and cancer.

Visible-light-induced bactericidal properties of a novel thiophene-based linear conjugated polymer/TiO2 heterojunction

“…In this study, the scavengers were isopropanol (1 mM) for OH, NaN 3 (1 mM) for 1 O 2 , L-ascorbic acid (1 mM) for O 2 À , EDTA-2Na (1 mM) for h + , and K 2 Cr 2 O 7 (1 mM) for e À . [51][52][53][54][55] The effect of scavengers on bacterial inactivation alone under illumination can be ignored, as demonstrated in Fig. 12a (light control).…”

Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques

“…Furthermore, since the fluorescence spectra of 2-OH-E + and E + are very similar, it is not possible to identify which ROS is responsible for the increasing in fluorescence. Although this particular problem can be solved by utilizing additional analytical techniques, including HPLC [83] or LC-MS [84] to separate the peaks prior to analyze the spectra of 2-OH-E + and E + , the observation that 2-OH-E + decomposes to E + , due to visible light exposure [83], makes its putative specificity towards •O 2 − , non-existent in photo-oxidation studies. Therefore, other fluorescence probes are better suited like the commercially available TEMPO-9-Ac [85,86], which contains a stable nitroxide radical (TEMPO), conjugated to a fluorescent acridine moiety.…”