A novel method of cell culture based on the microfluidic chip for regulation of cell density

Zhang, Fei; Zhang, Rongbiao; Wei, Mingji; Zhang, Yecheng

doi:10.1002/bit.27614

Cited by 7 publications

(5 citation statements)

References 58 publications

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“…Insufficient blood volume may lead to a lack of cell numbers, which can affect the preparation and examination of chromosomes. In cell culture, cell density is a critical factor influencing cell proliferation and division [22,23]. For instance, pre-B acute lymphoblastic leukemia cells (ALL3) exhibit poor or no growth under low-density conditions, yet thrive better under high initial cell density conditions.…”

Section: Discussionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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Objective: This study aims to investigate the impact of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis using response surface methodology (RSM). Methods: Peripheral blood samples from 30 volunteers were subjected to chromosomal analysis under different refrigeration duration periods (≤7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days) along with different blood volumes (0.2 mL, 0.3 mL, 0.4 mL, 0.5 mL, 0.6 mL, 0.7 mL, and 0.8 mL). The effects of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis were determined using the Chi-square test for trend, followed with Spearman's rank correlation coefficient, and RSM analysis to identify the optimal combination of refrigeration time and blood volume. Results: The refrigeration time within 10 days had a minor impact on the success rate, while refrigeration time more than 11 days significantly decreased the success rate. An increase in blood volume slightly improved the success rate. The success rate showed both linear and nonlinear changes with refrigeration time, while the effect of blood volume was primarily linear. The highest success rate was observed at a refrigeration time of ≤7 days and a blood volume of 0.8 mL. The interaction between refrigeration time and blood volume had a significant impact on the success rate. Conclusion: It is recommended to keep the refrigeration time of blood samples within 7 days and control the blood volume at 0.8 mL to maximize the success rate of chromosomal analysis.

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Section: Discussionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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“…In addition to the above, the regulation of cell density has been one of the problems in the development of microfluidic systems in the past. However, a study using interdigital electrode structures (IDES) recently examined the resulting impedance and recorded cell proliferation rates using feedback controllers to maintain and stabilize cell density, which can be considered a great success in this field ( 138 ). Furthermore, industry reception is another important issue in the field of chip technology.…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Organ on a Chip: A Novel in vitro Biomimetic Strategy in Amyotrophic Lateral Sclerosis (ALS) Modeling

et al. 2022

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Amyotrophic lateral sclerosis is a pernicious neurodegenerative disorder that is associated with the progressive degeneration of motor neurons, the disruption of impulse transmission from motor neurons to muscle cells, and the development of mobility impairments. Clinically, muscle paralysis can spread to other parts of the body. Hence it may have adverse effects on swallowing, speaking, and even breathing, which serves as major problems facing these patients. According to the available evidence, no definite treatment has been found for amyotrophic lateral sclerosis (ALS) that results in a significant outcome, although some pharmacological and non-pharmacological treatments are currently applied that are accompanied by some positive effects. In other words, available therapies are only used to relieve symptoms without any significant treatment effects that highlight the importance of seeking more novel therapies. Unfortunately, the process of discovering new drugs with high therapeutic potential for ALS treatment is fraught with challenges. The lack of a broad view of the disease process from early to late-stage and insufficiency of preclinical studies for providing validated results prior to conducting clinical trials are other reasons for the ALS drug discovery failure. However, increasing the combined application of different fields of regenerative medicine, especially tissue engineering and stem cell therapy can be considered as a step forward to develop more novel technologies. For instance, organ on a chip is one of these technologies that can provide a platform to promote a comprehensive understanding of neuromuscular junction biology and screen candidate drugs for ALS in combination with pluripotent stem cells (PSCs). The structure of this technology is based on the use of essential components such as iPSC- derived motor neurons and iPSC-derived skeletal muscle cells on a single miniaturized chip for ALS modeling. Accordingly, an organ on a chip not only can mimic ALS complexities but also can be considered as a more cost-effective and time-saving disease modeling platform in comparison with others. Hence, it can be concluded that lab on a chip can make a major contribution as a biomimetic micro-physiological system in the treatment of neurodegenerative disorders such as ALS.

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“…The cell medium was injected into the microfluidic chip and incubated for 8 h for adhesion. The initial cell density regulated to the same (~80%) using automatic control system (F. Zhang et al, 2020)…”

Section: Cell Detachment Assaymentioning

confidence: 99%

“…Because the normalized impedance is approximately linearly correlated with the number of cells attached to the electrodes Xiao et al, 2002). To improve repeatability of our approach, we firstly regulated cell density to the same utilizing the automatic control system proposed in our previous work (F. Zhang et al, 2020). The results show that compared with the cell counting method and the multiple linear regression (MLR) method, our fusion method based on polynomial regression has significantly improved the accuracy of cell viability heterogeneity assessment.…”

Section: Introductionmentioning

confidence: 97%

Evaluating cell viability heterogeneity based on information fusion of multiple adhesion strengths

Wei

Zhang

et al. 2021

Biotech & Bioengineering

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Cell viability evaluation is significantly meaningful for cellular assays. Some cells with weak viability are easily killed in the detection of anticancer drugs, while others with strong viability survive and proliferate, ultimately leading to the treatment failure or the inaccuracy of biological assays. Accurately evaluating cell viability heterogeneity still remains difficult. This article proposed a multiphysical property information fusion method for evaluating cell viability heterogeneity based on polynomial regression in a single-channel integrated microfluidic chip. In this method, adhesion strengths τ N , that are defined as the magnitude of shear stress needed to detach (100 − N) % of cell population, were extracted as the independent variables of polynomial regression model by calculating the nonlinear fitting of the impedance-response curves for shear stress (cell detachment assay). Besides, by calculating the nonlinear fitting of the drug dose-response curves for cancer cells (IC 50 assay), the half-maximal inhibitory concentration (IC 50 ) was extracted as the dependent variables of polynomial regression model. The results show that the mean relative error of our fusion method averagely reduces by 6.04% and 62.79% compared with the multiple linear regression method and the cell counting method. Moreover, a simplified theoretical model used to describe the quantitative relationship between cell viability and its adhesion strengths was built to provide a theoretical basis for our fusion method.

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A novel method of cell culture based on the microfluidic chip for regulation of cell density

Cited by 7 publications

References 58 publications

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Organ on a Chip: A Novel in vitro Biomimetic Strategy in Amyotrophic Lateral Sclerosis (ALS) Modeling

Evaluating cell viability heterogeneity based on information fusion of multiple adhesion strengths

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