A novel method to construct a third-generation biosensor: self-assembling gold nanoparticles on thiol-functionalized poly(styrene-co-acrylic acid) nanospheres

Xu, Shiyi; Han, Xijiang

doi:10.1016/j.bios.2003.09.007

Cited by 103 publications

(24 citation statements)

References 15 publications

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“…The biosensor displayed an excellent and quick electrocatalytic response to the reduction of H 2 O 2 . A novel method for fabrication of horseradish peroxidase (HRP) biosensor has been developed by self-assembling gold nanoparticles on thiol-functionalized poly(styrene-coacrylic acid) (St-co-AA) nanospheres (Xu et al, 2004). At first, a cleaned gold electrode was immersed in thiol-functionalized poly(St-co-AA) nanosphere latex prepared by emulsifierfree emulsion polymerization of St with AA and function with dithioglycol to assemble the nanospheres, then gold nanoparticles were chemisorbed onto the thiol groups.…”

Section: Horseradish Peroxidasementioning

confidence: 99%

Nanobiosensors for health care

Atta¹,

Galal²,

Ali³

2011

Biosensors for Health, Environment and Biosecurity

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Section: Horseradish Peroxidasementioning

confidence: 99%

Nanobiosensors for health care

Atta¹,

Galal²,

Ali³

2011

Biosensors for Health, Environment and Biosecurity

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“…It is important to note that this system exhibits extraordinary selectivity and provides a simple means for colorimetric, one-pot detection of a target oligonucleotide in the presence of a mixture of oligonucleotides with sequences differing by one nucleotide, regardless of position, in the target region (Storhoff, et. al., 1998;Tang, et al, 2004;Xu and Han, 2004 (Liu, et al, 2003). Alternatively Joseph H. O. Owino et al (Owino, et al, 2008) developed electrochemical immunosensor for detection of aflatoxin B1 (AFB1) by drop-coating of AuNPs on polythionine modified electrode surface.…”

Section: Figure 2 General Schematic Representation Of Biosensorsmentioning

confidence: 99%

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Sertova¹

2015

j. cent. eur. agric.

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A brief review of nanotechnology application in detection of mycotoxins and in agriculture sector was presented. Mycotoxins are secondary metabolites produced by fungi. Their toxicity is the reason for implementation of various screening methods to detect them. During the last years, the highlight was put on nanoscale materials included in biosensors, which were some of the smart devices used for determination of mycotoxins, and in agriculture sector. Over the next decade, the progress of nanotechnology will demonstrated a way to improve detection of contaminated feed and food. To achieve this purpose the innovations of nanomaterials reported every year would be applied. In the paper, some of the applications developed by nanotechnology that would contribute to the implementation of new tools for analysis of mycotoxins and agricultural products were discussed.Keywords: agriculture, biosensors, mycotoxins, nanoparticles, nanotechnology Резюме Представен е кратък преглед за приложение на нанотехнологиите за откриване на микотоксини, както и в аграрния сектор. Микотоксините са вторични метаболити, произвеждани от гъбички. Тяхната токсичност е причина за прилагане на различни методи за откриването им. През последните години акцента е бил поставен върху нано материали, вградени в биосензори, които са част от модерните устройства, използвани за откриване на микотоксини, а също и в аграрния сектор. През следващото десетилетие ще бъде доказван напредъка на нанотехнологиите да бъдат използвани като метод за детекция на замърсени фуражи и храни. За постигането на тази цел всяка година ще се следят новостите при наноматериалите. В статията са обсъдени някои от приложенията,разработени с помощта на нанотехнологиите, които биха допринесли за изграждането на нови устройства за анализ на микотоксини и селскостопански продукти.

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“…Since nanoparticles were first used for immobilization of biomolecules, gold nanoparticles have been extensively used as a matrix and cytochemical label for the immobilization and study of macromolecules such as protein, enzyme and antibodies (Storhoff et al 1998;Tang et al 2004;Xu and Han 2004). Modification of electrode surfaces with gold nanoparticles provides a microenvironment similar to that of the proteins in native systems and gives the protein molecules freedom in orientation (Liu et al 2003).…”

Section: Introductionmentioning

confidence: 99%

A new antibody immobilization strategy based on electro-deposition of gold nanoparticles and Prussian Blue for label-free amperometric immunosensor

Yuan

Chai

et al. 2006

Biotechnol Lett

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A novel and sensitive immunosensor has been developed by electro-depositing gold nanoparticles on to a Prussian Blue-modified glassy carbon electrode for determination of hepatitis B surface antigen (HBsAg). After the developed immunosensor was incubated with different concentrations of HBsAg samples at 37 degrees C for 15 min, the current response decreased with an increasing HBsAg concentration in the sample solution. The decreased percentage of the current was proportional to HBsAg concentration ranging from 2 to 300 ng HbsAg ml(-1) with a detection limit of 0.42 ng HbsAg ml(-1) (S/N = 3). Analytical results of 50 specimens using the developed immunosensor showed satisfactory agreement with those using ELISA, indicating the method to be a promising alternative for detecting HBsAg in clinical diagnosis.

show abstract

A novel method to construct a third-generation biosensor: self-assembling gold nanoparticles on thiol-functionalized poly(styrene-co-acrylic acid) nanospheres

Cited by 103 publications

References 15 publications

Nanobiosensors for health care

Nanobiosensors for health care

Application of nanotechnology in detection of mycotoxins and in agricultural sector

A new antibody immobilization strategy based on electro-deposition of gold nanoparticles and Prussian Blue for label-free amperometric immunosensor

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