A novel method to determine valnemulin in feedingstuffs for several animal species by liquid chromatography-electrospray tandem mass spectrometry

Abstract

A new method specific for the determination and unambiguous confirmation of the antibiotic valnemulin in feed for all animal species is described. Simple clean-up based on solvent extraction and liquid partition is carried out prior to determination by liquid chromatography coupled to electrospray tandem mass spectrometry on a hybrid QTRAP 4000 system, in multiple reaction monitoring mode. Valnemulin is licensed in the European Union as a veterinary drug for use in feed for pigs and rabbits, but unavoidable carryover in feed for non-target species should be monitored. The method is rapid and reliable, and was validated in-house at 10, 100 and 750 ng g, evaluating the analytical performances according to Commission Regulation (EC) No. 882/2004. Good mean recoveries from 79.7% to 92.6%, and within-laboratory reproducibility RSDs, ranging from 6.2% to 12.1%, were measured; the limit of quantification at 10.0 ng g also allows for sensitive evaluation of undesirable carryover in feed for non-target species. The results of monitoring 24 compound feeds for several animal species are also reported.

P6 | Potential therapeutic effect of ozone water on inflammatory skin symptoms and periodontal diseases in canine

“…Introduction: Amoxicillin (AMX) is a semisynthetic penicillin used in the treatment of a various infections caused by pathogenic bacteria. 1,2 There are diverse methods to develop a long-acting formulation and in this study we hypothesized that delayed absorption might lead delayed elimination of drug substance. The aim of this study was to describe a PK profile of intramuscular (IM) AMX formula with different tween80 contents in dogs.…”