A novel method to obtain protein release from porous polymer scaffolds: emulsion coating

Sohier, Jérôme; Haan, RE; Groot, K. de; Bezemer, J.M.

doi:10.1016/s0168-3659(02)00350-4

Cited by 72 publications

(52 citation statements)

References 40 publications

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“…High n values indicate a high inter-pore connection. As was previously reported for single emulsion coated scaffolds [16], the coated layers partly filled the pores and consequently decreased porosity. The increase of scaffold permeability after the first coating is due to the opening of (partly) closed pores which were present in the prefabricated compression molded-salt leached scaffolds [16].…”

Section: Porous Scaffolds Releasing Two Proteinssupporting

confidence: 80%

“…As was previously reported for single emulsion coated scaffolds [16], the coated layers partly filled the pores and consequently decreased porosity. The increase of scaffold permeability after the first coating is due to the opening of (partly) closed pores which were present in the prefabricated compression molded-salt leached scaffolds [16]. When the emulsion flows through the scaffold during the coating process, the solvent present in the emulsion dissolves the thin polymeric, membranes between the pores.…”

Section: Porous Scaffolds Releasing Two Proteinssupporting

confidence: 80%

“…The lysozyme concentration was further confirmed with another detection method based on a Micrococcus Lysodeikticus assay [16,27]. To 150 Al of the lysozyme release medium, a suspension of M. Lysodeikticus (100 Al, 2.3 mg/ml), was added in a 96-wells microplate.…”

Section: In Vitro Protein Releasementioning

confidence: 98%

“…The prefabricated scaffolds were obtained by compression molding-salt leaching method, using salt crystals sieved between 400 and 600 Am and 300PEGT55PBT45 copolymer granules, as reported in detail elsewhere [16].…”

Section: Preparation Of Double Emulsion-coated Scaffoldsmentioning

confidence: 99%

“…It is therefore important to be able to modulate precisely the amount and release rate of bioactive compound released from porous structures. A suitable method for this purpose, based on the coating of a protein-containing polymeric emulsion on top of a prefabricated scaffold, has been reported [16].…”

Section: Introductionmentioning

confidence: 99%

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Dual release of proteins from porous polymeric scaffolds

Sohier¹,

Vlugt

Cabrol³

et al. 2006

Journal of Controlled Release

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To create porous scaffolds releasing in a controlled and independent fashion two different proteins, a novel approach based on protein-loaded polymeric coatings was evaluated. In this process, two water-in-oil emulsions are forced successively through a prefabricated scaffold to create coatings, containing each a different protein and having different release characteristics. In a first step, a simplified three-layered system was designed with model proteins (myoglobin and lysozyme). Poly(ether -ester) multiblock copolymers were chosen as polymer matrix, to allow the diffusion of proteins through the coatings. The model system showed the independent release of the two proteins. The myoglobin release was tailored from a burst to a linear release still on-going after 60 days, while the lysozyme release rate was kept constant. Macro-porous scaffolds, with a porosity of 59 vol.%, showed the same ability to control the release rate of the model proteins independently. The relation between the coatings properties and their release characteristics were investigated with the use of a mathematical diffusion model based on Fick's second law. It confirmed that the multiple coated scaffolds are biphasic system, where each coating controls the release of the protein that it contains. This approach could be of value for tissue engineering applications. D

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Section: Porous Scaffolds Releasing Two Proteinssupporting

confidence: 80%

Section: Porous Scaffolds Releasing Two Proteinssupporting

confidence: 80%

Section: In Vitro Protein Releasementioning

confidence: 98%

Section: Preparation Of Double Emulsion-coated Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dual release of proteins from porous polymeric scaffolds

Sohier¹,

Vlugt

Cabrol³

et al. 2006

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

show abstract

Controlled Growth Factor Delivery for Tissue Engineering

2009

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Growth factors play a crucial role in information transfer between cells and their microenvironment in tissue engineering and regeneration. They initiate their action by binding to specific receptors on the surface of target cells and the chemical identity, concentration, duration, and context of these growth factors contain information that dictates cell fate. Hence, the importance of exogenous delivery of these molecules in tissue engineering is unsurprising, considering their importance for tissue regeneration. However, the short half-lives of growth factors, their relatively large size, slow tissue penetration, and their potential toxicity at high systemic levels, suggest that conventional routes of administration are unlikely to be effective. In this review, we provide an overview of the design criteria for growth factor delivery vehicles with respect to the growth factor itself and the microenvironment for delivery. We discuss various methodologies that could be adopted to achieve this localized delivery, and strategies using polymers as delivery vehicles in particular.

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Preparation and drug release activity of scaffolds containing collagen and poly(caprolactone)

Prabu

Dharmaraj

Aryal

et al. 2006

J Biomedical Materials Res

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A new biodegradable polymeric scaffold was prepared by using collagen and poly(caprolatctone) (PCL). These scaffolds were found to be soft, spongy, and transparent in nature and characterized by thermogravimetric analysis and FTIR spectrum. To these biodegradable polymeric scaffolds, antibiotic drugs namely amikacin and gentamycin were incorporated separately to study their release pattern from scaffolds. Amikacin and gentamycin release activity of the scaffolds containing a constant quantity of collagen but different quantities of PCL were studied at various time intervals viz. 1, 4, 24, and 48 h by measuring the optical density at 257 and 255 nm, respectively.

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A novel method to obtain protein release from porous polymer scaffolds: emulsion coating

Cited by 72 publications

References 40 publications

Dual release of proteins from porous polymeric scaffolds

Dual release of proteins from porous polymeric scaffolds

Controlled Growth Factor Delivery for Tissue Engineering

Preparation and drug release activity of scaffolds containing collagen and poly(caprolactone)

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