A novel method to predict protein aggregations using two-dimensional native protein microfluidic chip electrophoresis

Yu, Shiyong; Xu, Jing; Huang, Kunjie; Chen, Juan; Duan, Jinyan; Xu, Yuanqing; Qing, Hong; Li, Geng; Deng, Yulin

doi:10.1039/c6ay02011a

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…The advantages of microfluidic electrophoresis include low-sample requirements, cost-efficiency, rapid detection, as well as convenient integration with sensing modules [123]. Some examples of types of electrophoretic methods that have been implemented in microfluidic devices include gel electrophoresis, capillary electrophoresis, and dielectrophoresis [124,125]. Figure 15.…”

Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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“…Neurological diseases including multiple sclerosis [104], epilepsy [105,106], bipolar disorder [107,108], and Alzheimer's disease [109][110][111][112] were investigated…”

Section: Neurological Diseasesmentioning

confidence: 99%

“…The time for sample-in-answer-out was 3.5 min including analyte separation in 30 s.2.3.4 Alzheimer's diseaseAlzheimer's disease is a neurodegenerative disease that is associated with elevated levels of phosphorylated tau protein and β-amyloids, and the toxic protein aggregation in the brain. An on-chip hyphenation of isoelectric focusing and zone electrophoresis strategy was applied to study protein aggregation[109]. The β-amyloid peptides were investigated by immunocapture of the peptides from cerebrospinal fluids and subsequent electrophoretic enrichment and separation prior to the fluorescence[110] and mass spectrometric detection[111].…”

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confidence: 99%

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Wuethrich

Quirino

2019

Analytica Chimica Acta

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A core element in clinical diagnostics is the data interpretation obtained through the analysis of patient samples. To obtain relevant and reliable information, a methodological approach of sample preparation, separation, and detection is required. Traditionally, these steps are performed independently and stepwise. Microchip capillary electrophoresis (MCE) can provide rapid and high-resolution separation with the capability to integrate a streamlined and complete diagnostic workflow suitable for the point-of-care setting. Whilst standard clinical diagnostics methods normally require hours to days to retrieve specific patient data, MCE can reduce the time to minutes, hastening the delivery of treatment options for the patients. This review covers the advances in MCE for disease detection from 2008 to 2017. Miniaturised diagnostic approaches that required an electrophoretic separation step prior to the detection of the biological samples are reviewed. In the two main sections, the discussion is focused on the technical setup used to suit MCE for disease detection and on the strategies that have been applied to study various diseases. Throughout these discussions MCE is compared to other techniques to create context of the potential and challenges of MCE. A comprehensive table categorised based on the studied disease using MCE is provided. We also comment on future challenges that remain to be addressed.

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“…The analysis employed a two-dimensional separation approach involving a combination of isoelectric focusing and capillary zone electrophoresis. 93 The combined platform allowed the time-course of α-synuclein aggregation to be studied and provided a strategy for real-time detection of early and intermediate species on the aggregation pathway. As part of the study it was shown that the chip could be used to analyse the effect of small molecules on the aggregate formation process and could as such serve as a platform for screening for potential aggregation inhibitors.…”

Section: Interactions Between Amyloid Structures and Other Moleculesmentioning

confidence: 99%

Microfluidic approaches for probing amyloid assembly and behaviour

et al. 2018

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The self-assembly of proteins into supramolecular structures and machinery underpins biological activity in living systems. Misassembled, misfolded and aggregated protein structures can, by contrast, have deleterious activity and such species are at the origin of a number of disease states ranging from cancer to neurodegenerative disorders. In particular, the formation of highly ordered protein aggregates, amyloid fibrils, from normally soluble peptides and proteins, is the common pathological hallmark of a range a group of over fifty protein misfolding disorders. Because of the critical role of the process in the aetiology of such disorders, as well as the quest to understand the basic principles of protein folding and misfolding, the amyloid phenomenon has become a central area of modern biomedical research. Advances in our knowledge of the physical properties of amyloid systems have, however, also highlighted the potential of amyloid structures in the context of materials science. In this review, we explore how microfluidic approaches can be used to study aspects of amyloid assembly and behaviour that are challenging to probe under bulk solution conditions. We discuss the use of volume confinement to probe very early events in the amyloid formation process. In addition, the well-defined fluid flow properties within channels with dimensions on the micron scale can be exploited to measure the physical properties of protein aggregates, such as their sizes and charges, to shed light on the physical and chemical parameters defining amyloid species. Moreover, the molecular species formed during aggregation reactions have physical dimensions spanning at least three orders of magnitude, and microfluidic techniques are well suited to work with analytes of such disparate dimensions. Furthermore, the flexibility of the design of microfluidic devices lends itself to adaptable experimental setups, including the study of protein self-assembly within living cells. Finally, we highlight the salient features of microfluidic experiments that facilitate probing complex biological systems, and discuss their use in the exploration of amyloids as a class of functional material.

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A novel method to predict protein aggregations using two-dimensional native protein microfluidic chip electrophoresis

Abstract: A microfluidic chip native protein electrophoresis was established to predict protein aggregation.

Cited by 11 publications

References 24 publications

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Microfluidic approaches for probing amyloid assembly and behaviour

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