A novel method to quantitate methylation of specific genomic regions.

Epigenetic phenotypes distinguish microsatellite-stable and -unstable colorectal cancers

“…To see whether the methylation patterns observed at the MLH1 promoter were restricted to this gene or, alternatively, were more widespread, we chose to study the calcitonin gene for comparison. The calcitonin gene is a well characterized methylation target in various malignancies including colon cancer, but, unlike MLH1, is not known to be associated with any selective advantage (10,(18)(19)(20). As shown in Fig.…”