A novel microbial biosensor based on cells of Gluconobacter oxydans for the selective determination of 1,3-propanediol in the presence of glycerol and its application to bioprocess monitoring

Abstract

Novel and selective microbial amperometric biosensors that use Gluconobacter oxydans cells to monitor the bacterial bioconversion of glycerol (Gly) to 1,3-propanediol (1,3-PD) are described. Two different mediators, ferricyanide and flexible polyvinylimidazole osmium functionalized polymer (Os-polymer), were employed to prepare two different microbial biosensors, both of which gave high detection performance. The good operational stabilities of both types of biosensor were underlined by the ability to detect 1,3-PD throughout 140 h of continuous operation. Both microbial biosensor systems showed excellent selectivity for 1,3-PD in the presence of a high excess of glycerol [selectivity ratios (1,3-PD/Gly) of 118 or 245 for the ferricyanide and Os-polymer systems, respectively]. Further, the robustness of each microbial biosensor was highlighted by the high reliability of 1,3-PD detection achieved (average RSD of standards<2%, and well below 4% for samples). The biosensor implementing the Os-polymer mediator exhibited high selectivity towards 1,3-PD detection and allowed moderate sample throughput (up to 12 h-1) when integrated into a flow system. This system was used to monitor the concentration of 1,3-PD during a real bioprocess. Results from biosensor assays of 1,3-PD in bioprocess samples taken throughout the fermentation were in a very good agreement with results obtained from reference HPLC assays (R2=0.999).

Improved Utilization of Crude Glycerol By-Product from Biodiesel Production

“…4.1 1,3-propanediol 1,3-propanediol is a typical and the oldest product of glycerol fermentation (Katrlík et al, 2007) Guo et al (2009) reported that Klebsiella pneumoniae CPSdeficient mutant (unable to produce capsular polysaccharides) in fed-batch fermentation was able to synthesize 78.13 g/L of 1,3-propanediol with yield and productivity of 0.53 mol/mol of glycerol and 1.95 g/L/h, respectively. Tang et al (2009) studied bioconversion of glycerol to 1,3-propanediol using engineered strain of Escherichia coli.…”

The biofuel elements on the basis of the nanocarbon materials

“…Thus, the thermally expanded graphite is a material with good electrical conductivity, high specific surface area up to 2000 m 2 /g, biocompatibility, chemical resistance in corrosive environments and can be a long time in the technical operation [37]. These qualities allow immobilization on the electrode TRG surface of the high superficial concentration of bacterial or their membrane fractions.…”

Selection and characterization of Clostridium bifermentans strains from natural environment capable of producing 1,3-propanediol under microaerophilic conditions

“…It is also used as a monomer for the production of biodegradable polymers (polyesters, polyether, polyurethanes, etc. ), cosmetics, lubricants, medicines, and as an intermediate for the synthesis of heterocyclic compounds (Regan and Crawford, 1994;Menzel et al, 1997;Katrlík et al, 2007;Biebl et al, 1999;. Recently, 1,3-PD has also been used as a monomer to synthesize a new type of polyester -polytrimethylene terephthalate (Biebl et al, 1999;Zeng and Biebl, 2002;Liu et al, 2007;Zhang et al, 2007).…”