A novel mode of air recycling favored stable operation of the aerobic granular sludge process via calcium accumulation

Zhang, Zhiming; Ji, Yatong; Cao, Runjuan; Yu, Zhuodong; Xu, Xiangyang; Zhu, Liang

doi:10.1016/j.cej.2019.04.083

Cited by 17 publications

(5 citation statements)

References 45 publications

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“…The AHL molecule 3OHC 8 -HSL was chosen because it is the most common quorum-sensing molecule for regulating the biofilm formation of Pseudomonas spp . except P. aeruginosa , which does not produce hydroxyl-HSL such as 3OHC 8 -HSL. − C 8 -HSL and C 6 -HSL are the AHLs produced by Burkholderia cenocepacia , which was found to not only inhabit the same environmental niches as P. aeruginosa but also form mixed biofilms with P. aeruginosa in the lungs of cystic fibrosis patients. , As shown in Figure , the biosensor had the highest EGFP production rate in the presence of 3OC 12 -HSL at the concentrations of 1.0 × 10 –8 and 1.0 × 10 –7 M, suggesting that the biosensor had a strong response to the cognate AHL from P. aeruginosa . The EGFP production rates induced by C 10 -HSL and 3OHC 10 -HSL were also substantial at both concentrations and were significantly higher than that induced by C 8 -HSL, 3OHC 8 -HSL, C 6 -HSL, or the negative control ( P ≤ 0.05, Table S1).…”

Section: Resultsmentioning

confidence: 96%

A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens

Wang

et al. 2021

ACS Synth. Biol.

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Water contamination by pathogenic bacteria is a major public health concern globally. Monitoring bacterial contamination in water is critically important to protect human health, but this remains a critical challenge. Engineered whole-cell biosensors created through synthetic biology hold great promise for rapid and cost-effective detection of waterborne pathogens. In this study, we created a novel whole-cell biosensor to detect water contamination by Pseudomonas aeruginosa and Burkholderia pseudomallei, which are two critical bacterial pathogens and are recognized as common causative agents for waterborne diseases. The biosensor detects the target bacterial pathogens by responding to the relevant quorum sensing signal molecules. Particularly, this study constructed and characterized the biosensor on the basis of the QscR quorum sensing signal system for the first time. We first designed and constructed a QscR on the basis of the sensing module in the E. coli host cell and integrated the QscR sensing module with a reporting module that expressed an enhanced green fluorescent protein (EGFP). The results demonstrated that the biosensor had high sensitivity in response to the quorum sensing signals of the target bacterial pathogens. We further engineered a biosensor that expressed a red pigment lycopene in the reporting module to produce a visible signal readout for the pathogen detection. Additionally, we investigated the feasibility of a paper-based assay by immobilizing the lycopene-based whole-cell biosensor on paper with the aim to build a prototype for developing portable detection devices. The biosensor would provide a simple and inexpensive alternative for timely and point-of-care detection of water contamination and protect human health.

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Section: Resultsmentioning

confidence: 96%

A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens

Wang

et al. 2021

ACS Synth. Biol.

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“…Scanning electron microscopy (SEM) (FEI QUANTA FEG 650, USA) was employed to characterize the surface morphology of sampled aggregates. Polysaccharide (PS) and protein (PN) in EPS were extracted using a heat extraction method as previously described . The total PS and PN contents in EPS were then quantified by a UV–vis spectrophotometer (SHIMADZU UV-2600, Japan) using a phenol-sulfuric acid method and modified Lowry colorimetric method, , respectively.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Bacterium–Phage Symbiosis in Attached Microbial Aggregates on a Membrane Surface Facing Elevated Hydraulic Stress

Tan,

Yu,

Huang

et al. 2023

Environ. Sci. Technol.

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Phages are increasingly recognized for their importance in microbial aggregates, including their influence on microbial ecosystem services and biotechnology applications. However, the adaptive strategies and ecological functions of phages in different aggregates remain largely unexplored. Herein, we used membrane bioreactors to investigate bacterium–phage interactions and related microbial functions within suspended and attached microbial aggregates (SMA vs AMA). SMA and AMA represent distinct microbial habitats where bacterial communities display distinct patterns in terms of dominant species, keystone species, and bacterial networks. However, bacteria and phages in both aggregates exhibited high lysogenicity, with 60% lysogenic phages in the virome and 70% lysogenic metagenome-assembled genomes of bacteria. Moreover, substantial phages exhibited broad host ranges (34% in SMA and 42% in AMA) and closely interacted with habitat generalist species (43% in SMA and 49% in AMA) as adaptive strategies in stressful operation environments. Following a mutualistic pattern, phage-carried auxiliary metabolic genes (pAMGs; 238 types in total) presumably contributed to the bacterial survival and aggregate stability. The SMA-pAMGs were mainly associated with energy metabolism, while the AMA-pAMGs were mainly associated with antioxidant biosynthesis and the synthesis of extracellular polymeric substances, representing habitat-dependent patterns. Overall, this study advanced our understanding of phage adaptive strategies in microbial aggregate habitats and emphasized the importance of bacterium–phage symbiosis in the stability of microbial aggregates.

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“…7. The more detailed information of different band assignments for the FT-IR spectral features are listed in Table S6 (Jia et al, 2017;Niu et al, 2016;Yin et al, 2015;Zhang et al, 2019). The vibration of main functional groups, including polysaccharide-related functional groups (CH stretching groups at 2930 cm −1 in CH 2 structure, CH plane bending vibration group at 1072 cm −1 ) and protein-related functional groups (amide CO at 1655 cm −1 , amide NH bending vibration at 1530 cm −1 ) in ALE with surfactants added, were weakened or strengthened to an extent.…”

Section: Functional Groups Adsorptionmentioning

confidence: 99%

Enhancing extraction of alginate like extracellular polymers (ALE) from flocculent sludge by surfactants

Hao

Gan

et al. 2022

Science of The Total Environment

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A novel mode of air recycling favored stable operation of the aerobic granular sludge process via calcium accumulation

Cited by 17 publications

References 45 publications

A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens

A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens

Enhanced Bacterium–Phage Symbiosis in Attached Microbial Aggregates on a Membrane Surface Facing Elevated Hydraulic Stress

Enhancing extraction of alginate like extracellular polymers (ALE) from flocculent sludge by surfactants

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