A Novel Model for Development, Organization, and Function of Gonadotropes in Fish Pituitary

Golan, Matan; Biran, Jakob; Levavi‐Sivan, Berta

doi:10.3389/fendo.2014.00182

Cited by 55 publications

(56 citation statements)

References 65 publications

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“…Significantly, Fsh cells also appeared to make direct contact with Lh cells along the ventral line of the pituitary. Similar observations have been made in zebrafish ( Danio rerio ) where Fsh cells were found at the periphery of Lh-cell clusters (49).…”

Section: Discussionsupporting

confidence: 84%

Gnrh1-induced responses are indirect in female medaka Fsh cells

Hodne

Fontaine

Ager-Wick

et al. 2019

Preprint

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Reproductive function in vertebrates is stimulated by gonadotropin-releasing hormone (GnRH) that controls the synthesis and release of the two pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). FSH and LH, which regulates different stages of gonadal development, are produced by two different cell types in the fish pituitary, in contrast to mammals and birds, thus allowing the investigation of their differential regulation. In the present work, we show by fluorescentin situhybridization that Lh cells in adult female medaka express Gnrh receptors, whereas Fsh cells do not. This is confirmed by patch clamp recordings and cytosolic Ca2+measurements on dispersed pituitary cells, where Lh cells, but not Fsh cells, respond to Gnrh1 by increased action potential frequencies and cytosolic Ca2+levels. In contrast, both Fsh and Lh cells are able to respond electrically and by elevating the cytosolic Ca2+levels to Gnrh1 in brain-pituitary tissue slices. Using Ca2+uncaging in combination with patch clamp recordings and cytosolic Ca2+measurements, we show that Fsh and Lh cells form homo- and heterotypic networks in the pituitary. Taken together, these results show that the effects of Gnrh1 on Fsh release in adult female medaka is indirect, likely mediated via Lh cells.

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Section: Discussionsupporting

confidence: 84%

Gnrh1-induced responses are indirect in female medaka Fsh cells

Hodne

Fontaine

Ager-Wick

et al. 2019

Preprint

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“…The anterior pituitary is a complex endocrine gland that secretes multiple hormones to control lactation, growth, reproduction, and homeostasis. Accumulating evidence indicates that in both mammals and teleost fish, some pituitary cell types are organized into complex threedimensional homotypic and heterotypic networks (Bonnefont et al, 2005;Budry et al, 2011;Edwards et al, 2017;Golan et al, 2016a;Golan et al, 2016b;Mollard et al, 2012). In teleost fish, the different endocrine cell types are clustered (Golan et al, 2014;Levavi-Sivan et al, 2010;Weltzien et al, 2004), whereas in mammalian pituitaries the different pituitary cell types are distributed in a more mosaic-like pattern (Musumeci et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Gonadotropes in medaka grow long extensions with varicosity-like swellings, projecting towards each other and blood vessels

Grønlien

Fontaine

Hodne

et al. 2019

Preprint

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___________________________________________________________________________Abbreviations: ACTH, Adrenocorticotropic hormone (mammals); DiI, lipophilic carbocyanine dye; FSH/Fsh, Follocle-stimulating hormone protein (mammals/fish); GFP, green fluorescent protein; GnRH/Gnrh, Gonadotropin releasing hormone protein (mammals/fish); IQR, interquartile range; LH/Lh, Luteinizing hormone protein (mammals/fish); Lhβ/Lhβ, Luteinizing hormone β subunit protein (mammals/fish); Lhb/lhb, Luteinizing hormone β subunit gene (mammals/fish); Tamra, 5/6-carboxy-tetramethyl-rhodamine succinimidyl ester AbstractAccumulating evidence in the scientific literature indicates that some pituitary cell types are organized in complex networks. Previous observations have indicated that this may also be the case in medaka (Oryzias latipes), where long cellular extensions with varicosity-like swellings are formed by luteinizing hormone (Lh)-producing gonadotropes expressing green fluorescent protein. In this study, immunofluorescence of intact pituitaries reveal that Lh beta polypeptides are mainly located in the varicosity-like swellings and at the extremity of the extensions. Some extensions approach nearby Lh-producing cells, and other extensions are in close contact with blood vessels. To investigate whether these extensions may contribute to network formation, we followed their development using confocal and fluorescent microscopy on primary cultures.During the first two days in culture, the extensions initiated the formation of homotypic cellular networks and clustering. The extensions were classified as either major or minor. Major extensions were several cell diameters long, dependent on microtubules, and displaying varicosity-like swellings at regular intervals. Minor extensions typically protruded from the major, were significantly shorter and thinner, and dependent on actin. The swellings were dependent on both microtubules and actin. Flash photolysis of caged Ca 2+ showed that the signal was propagated along the major extensions, intensifying in each swelling, indicating a continuous structure. However, the Ca 2+ signal did not transfer to the next cell in the network, but was transferred between cells merged at their somas. In summary, Lh-producing gonadotropes in medaka display a complex cellular structure of extensions, possibly linked to communication with blood vessels and/or other gonadotrope cells.

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“…Up to now, only a few transgenic lines have been established in tilapia (Golan and Levavisivan 2013;Golan et al 2014). Most transgenic research has been performed on the F0 generation (Kaneko et al 2015;Li et al 2015).…”

Section: Transgenic Technologymentioning

confidence: 99%

A Review of Genetic Advances Related to Sex Control and Manipulation in Tilapia

Chen

Fan

Tan

et al. 2017

J World Aquaculture Soc

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Tilapias are the second most farmed fish worldwide. Production of tilapia has quadrupled over the past decade because of the ease with which they are cultured, their marketability, and the stability of market prices. Continued increases in tilapia production must be based on sustainable practices. Excessive reproduction during growout has been a major problem in tilapia aquaculture and has been addressed by culturing monosex (all‐male) populations. The best way to obtain monosex populations is through control of sexual development. To produce genetically male tilapia, researchers can arrange matings using sex‐specific DNA markers, create hybrids between tilapia strains with different sex determination loci, or they can alter the innate genetic traits of a species. Improved varieties have, in many cases, facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield. In this review, we focus on the application of marker‐assisted breeding, cross‐breeding, and genetic modification technologies to control sexual development in tilapia.

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A Novel Model for Development, Organization, and Function of Gonadotropes in Fish Pituitary

Cited by 55 publications

References 65 publications

Gnrh1-induced responses are indirect in female medaka Fsh cells

Gnrh1-induced responses are indirect in female medaka Fsh cells

Gonadotropes in medaka grow long extensions with varicosity-like swellings, projecting towards each other and blood vessels

A Review of Genetic Advances Related to Sex Control and Manipulation in Tilapia

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