1998
DOI: 10.1038/sj.pcan.4500233
|View full text |Cite
|
Sign up to set email alerts
|

A novel monoclonal antibody 107-1A4 with high prostate specificity: generation, characterization of antigen expression, and targeting of human prostate cancer xenografts

Abstract: Monoclonal antibodies with high speci®city for prostate tissue are of interest for prostate cancer research and treatment. Reactivity and speci®city of a new murine monoclonal antibody, 107-1A4, was assessed by immunohistochemistry, ELISA and indirect immuno¯uorescence (IDIF). 107-1A4 stained all normal and malignant prostate tissue specimens while reactivity to non-prostate tissue was limited to the tubules of the normal kidney and renal cell carcinoma. Twenty two human cell lines were included in the reactiv… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

1
21
0

Year Published

2001
2001
2021
2021

Publication Types

Select...
4
1

Relationship

0
5

Authors

Journals

citations
Cited by 19 publications
(22 citation statements)
references
References 7 publications
1
21
0
Order By: Relevance
“…We concluded that the target antigen was distinct from PSA, PAP and PSP-94, since it failed to react with these purified proteins. 2 By comparing the reactivity of 107-1A4 with those of other MAbs on tissues and cell lines, we eliminated several other known antigens as potential targets. The distribution of the 107-1A4 antigen in prostate tissue was similar to that reported for PSMA by immunohistochemical staining; however, there was an apparent difference in the reported regulation of PSMA by androgens and that we observed for the 107-1A4 antigen, in both cell lines and xenografts.…”
Section: Discussionmentioning
confidence: 99%
See 4 more Smart Citations
“…We concluded that the target antigen was distinct from PSA, PAP and PSP-94, since it failed to react with these purified proteins. 2 By comparing the reactivity of 107-1A4 with those of other MAbs on tissues and cell lines, we eliminated several other known antigens as potential targets. The distribution of the 107-1A4 antigen in prostate tissue was similar to that reported for PSMA by immunohistochemical staining; however, there was an apparent difference in the reported regulation of PSMA by androgens and that we observed for the 107-1A4 antigen, in both cell lines and xenografts.…”
Section: Discussionmentioning
confidence: 99%
“…2 Solutions (100 L/well) containing biotinylated MAb 107-1A4 or biotinylated MOPC-21 at 2 g/mL with and without competing, unlabeled MAbs at 50 g/mL in 2% BSA/PBS were added to microtiter wells, which had been coated with LNCaP cells. 2 After incubation for 1 hr at 37°C, the plates were washed with TTBS, and 50 L/well of ABC-alkaline phosphatase (Vectastain; Vector, Burlingame, CA), prepared according to the manufacturer's instructions, was added. After a 15 min incubation at room temperature, the plates were washed, and bound conjugate was detected with para-nitrophenylphosphate.…”
Section: Competitive Bindingmentioning
confidence: 99%
See 3 more Smart Citations