A novel monopartite begomovirus and satellites associated with yellow mosaic disease of Sida spp. in India

Chahwala, Fenisha D.; Singh, Devraj; Kumar, Ravinder; Rathore, Sangeeta; Yadav, Brijesh; Singh, Bijendra; Singh, Achuit K.

doi:10.1007/s00705-020-04843-9

Cited by 4 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, multiple infections of weeds by begomoviruses can lead to recombination events that could originate new BGVs species, as this is a common mechanism used by these viruses ( Padidam, Sawyer & Fauquet, 1999 ; Jovel et al, 2004 ; Rojas et al, 2005b ; Graham, Martin & Roye, 2009 ; Tavares et al, 2012 ; Ferro et al, 2017 ). Weeds from the Sida and Malvastrum genera are particularly relevant hosts due to their function as reservoirs and are natural hosts for 37 and 11 species, respectively, making them the group of weeds that harbor the most begomoviral species ( Stewart et al, 2014 ; Alabi et al, 2016 ; Fiallo-Olivé et al, 2021 ; Chahwala et al, 2021 ). Therefore, plants from the genera Sida and Malvastrum pose a risk to agriculture as they can act as incubators for new BGVs species due to the frequent coexistence of diverse viruses within the same host ( Jovel et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Sida chlorotic leaf virus: a new recombinant begomovirus found in non-cultivated plants and Cucumis sativus L

García-Rodríguez

Partida-Palacios

Regla-Márquez³

et al. 2023

PeerJ

View full text Add to dashboard Cite

Background Begomoviruses are circular single-stranded DNA plant viruses that cause economic losses worldwide. Weeds have been pointed out as reservoirs for many begomoviruses species, especially from members of the Sida and Malvastrum genera. These weeds have the ability to host multiple begomoviruses species simultaneously, which can lead to the emergence of new viral species that can spread to commercial crops. Additionally, begomoviruses have a natural tendency to recombine, resulting in the emergence of new variants and species. Methods To explore the begomoviruses biodiversity in weeds from genera Sida and Malvastrum in Colima, México, we collected symptomatic plants from these genera throughout the state. To identify BGVs infecting weeds, we performed circular DNA genomics (circomics) using the Illumina platform. Contig annotation was conducted with the BLASTn tool using the GenBank nucleotide “nr” database. We corroborated by PCR the presence of begomoviruses in weeds samples and isolated and sequenced the complete genome of a probable new species of begomovirus using the Sanger method. The demarcation process for new species determination followed the International Committee on Taxonomy of Viruses criteria. Phylogenetic and recombination analyses were implemented to infer the evolutionary relationship of the new virus. Results We identified a new begomovirus species from sida and malvastrum plants that has the ability to infect Cucumis sativus L. According to our findings, the novel species Sida chlorotic leaf virus is the result of a recombination event between one member of the group known as the Squash leaf curl virus (SLCV) clade and another from the Abutilon mosaic virus (AbMV) clade. Additionally, we isolated three previously identified begomoviruses species, two of which infected commercial crops: okra (Okra yellow mosaic Mexico virus) and cucumber (Cucumber chlorotic leaf virus). Conclusion These findings support the idea that weeds act as begomovirus reservoirs and play essential roles in begomovirus biodiversity. Therefore, controlling their populations near commercial crops must be considered in order to avoid the harmful effects of these phytopathogens and thus increase agricultural efficiency, ensuring food and nutritional security.

show abstract