A novel mutant allele uncouples brassinosteroid-dependent and independent functions of BRI1

Holzwart, Eleonore; Gloeckner, Nina; Hoefte, H.; Harter, Klaus; Wolf, Sebastián

doi:10.1101/605923

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…The RECEPTOR-LIKE PROTEIN 44 (RLP44) was previously described to play a role in cell wall integrity sensing via modulation of brassinosteroid-mediated responses (Wolf et al, 2014). It directly interacts with both BRI1 and BAK1 (Wolf et al, 2014;Holzwart et al, 2018) (Holzwart et al, 2019). A recent study also showed that RLP44 plays a role in vascular development, which involves phytosulfokine hormone signalling (Holzwart et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Three-fluorophore FRET enables the analysis of ternary protein association in living plant cells

Glöckner

Oven-Krockhaus

Rohr

et al. 2019

Preprint

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Integration of signalling on the cellular level is essential for the survival of organisms.Protein-protein interaction studies provide valuable insights in these signalling events.One of the best understood signalling pathways in plants is the brassinosteroid (BR) hormone signalling pathway, which is mediated by the receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) with its co-receptor BRI1-ASSOCIATED KINASE (BAK1). Both BRI1 and BAK1 have been shown to interact with RECEPTOR LIKE PROTEIN 44 (RLP44), which was implicated in cell wall integrity sensing by modulation of BL signalling. Here we provide evidence by quantitative in vivo three-fluorophore FRET-FLIM measurements, that RLP44, BRI1 and BAK1 form a trimeric complex in the plasma membrane of N. benthamiana leaf cells, with an estimated distance between them below 15 nm. The immune receptor FLAGELLIN SENSING 2 (FLS2), which is also a receptor-like kinase like BRI1, is not integrated in a similar complex with RLP44 and BAK1. Our study supports that BRI1 and FLS2 are localized in distinct nanodomains in the PM. As the fluorescence lifetime of the donor is monitored, our method circumvents the extensive calculations necessitated by intensity-based FRET interaction assays and thus provides a feasible base for studying the sub-compartmentalization in the plasma membrane of living plant cells with a nanoscale resolution.

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Section: Introductionmentioning

confidence: 99%

Three-fluorophore FRET enables the analysis of ternary protein association in living plant cells

Glöckner

Oven-Krockhaus

Rohr

et al. 2019

Preprint

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Optimal BR signalling is required for adequate cell wall orientation in the Arabidopsis root meristem

Sela

Fridman

et al. 2021

Preprint

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The plant brassinosteroid hormones (BRs) regulate growth in part through altering the properties of the cell wall, the extracellular matrix of plant cells. Conversely, cell wall signalling connects the state of cell wall homeostasis to the BR receptor complex and modulates BR activity. Here we report that both pectin-triggered cell wall signalling and impaired BR signalling result in altered cell wall orientation in the Arabidopsis root meristem. BR-induced defects in the orientation of newly placed walls are associated with aberrant localization of the cortical division zone but with normal specification of its positioning. Tissue-specific perturbations of BR signalling revealed that the cellular malfunction is unrelated to previously described whole organ growth defects. Thus, tissue type separates the pleiotropic effects of cell wall/BR signals and highlights their importance during cell wall placement.

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Deviating from the Beaten Track: New Twists in Brassinosteroid Receptor Function

Wolf¹

2020

IJMS

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A key feature of plants is their plastic development tailored to the environmental conditions. To integrate environmental signals with genetic growth regulatory programs, plants rely on a number of hormonal pathways, which are intimately connected at multiple levels. Brassinosteroids (BRs), a class of plant sterol hormones, are perceived by cell surface receptors and trigger responses instrumental in tailoring developmental programs to environmental cues. Arguably, BR signalling is one of the best-characterized plant signalling pathways, and the molecular composition of the core signal transduction cascade seems clear. However, BR research continues to reveal new twists to re-shape our view on this key signalling circuit. Here, exciting novel findings pointing to the plasma membrane as a key site for BR signalling modulation and integration with other pathways are reviewed and new inputs into the BR signalling pathway and emerging “non-canonical” functions of the BR receptor complex are highlighted. Together, this new evidence underscores the complexity of plant signalling integration and serves as a reminder that highly-interconnected signalling pathways frequently comprise non-linear aspects which are difficult to convey in classical conceptual models.

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A novel mutant allele uncouples brassinosteroid-dependent and independent functions of BRI1

Cited by 3 publications

References 31 publications

Three-fluorophore FRET enables the analysis of ternary protein association in living plant cells

Three-fluorophore FRET enables the analysis of ternary protein association in living plant cells

Optimal BR signalling is required for adequate cell wall orientation in the Arabidopsis root meristem

Deviating from the Beaten Track: New Twists in Brassinosteroid Receptor Function

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