A Novel NAD&lt;sup&gt;+&lt;/sup&gt;/MWNTs Nanocomposite-Based Electrochemical Biosensor for Measuring Mevalonic Acid

Palangsuntikul, Rungtiva; Pakapongpan, Saithip; Khownarumit, Porntip; Surareungchai, Werasak

doi:10.4028/www.scientific.net/kem.605.388

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“…The peak to peak potential separation ( E p ) was about 214 mV vs. Ag/AgCl and the ratio of the redox peak current I pa /I pc was about 1 suggesting that the electrochemical reaction is quasi-reversible as a result of the construction of the nanocomposite and the small peak to peak separation values indicate a fast electron transfer rate resulting from the strong interaction between NAD + and MWCNTs. Effect of scan rates.-Effect of scan rates on peak current of the NAD + /MWCNTs nanocomposite modified SPCE was investigated in 0.1 M PBS pH 7.0 at different scan rates 32 (see Figure S2). The anodic peak currents (I pa ) and cathodic peak currents (I pc ) increased linearly with increasing scan rates from 10 to 100 mV/s.…”

Section: Electrochemical Properties Of Nadmentioning

confidence: 99%

“…Effect of scan rates.-Effect of scan rates on peak current of the NAD + /MWCNTs nanocomposite modified SPCE was investigated in 0.1 M PBS pH 7.0 at different scan rates 32 (see Figure S2). The anodic peak currents (I pa ) and cathodic peak currents (I pc ) increased linearly with increasing scan rates from 10 to 100 mV/s.…”

Section: Electrochemical Properties Of Nadmentioning

confidence: 99%

“…Hence, there is a need for developing a new method, which should be rapid, sensitive, specific, cost effective and preferable at the point of sample collection. From our preliminary report, 32 we demonstrated the cyclic voltammetry behavior of modified electrode which successfully showed characteristic of an efficient catalysis to MA. For this work, we aim to further develop a NAD + /MWCNTs-based amperometric biosensor through noncovalent attachment of NAD + /MWCNTs nanocomposite on a screen-printed carbon electrode (SPCE) to create innovative disposable biosensor, which is more reliable than the conventional techniques to measure MA concentration.…”

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Development of Mevalonic Acid Biosensor Using Amperometric Technique Based on Nanocomposite of Nicotinamide Adenine Dinucleotide and Carbon Nanotubes

Poo-arporn

Pakapongpan

Khownarumit

et al. 2017

J. Electrochem. Soc.

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This study reports the development of an amperometric biosensor for quantification of mevalonic acid (MA), which is the first intermediate of HMG-CoA reductase in the isoprenoids biosynthesis pathway and therefore a useful indicator of HMGR activity. This method offers important advantages over previous reports because no radiolabeled substrates or expensive techniques are required, and time of analysis is relatively short. Self-assembled NAD + onto multiwall-carbon nanotubes (MWCNTs) was synthesized for a biosensing system. Adsorption of NAD + on MCWNTs was characterized by X-ray photoelectron spectroscopy (XPS) technique. This biosensor was constructed by modifying a screen-printed carbon electrode (SPCE) with NAD + /MWCNTs nanocomposite. The electrochemical and electrocatalytic behaviors of the modified electrode were studied using amperometry and cyclic voltammetry (CV). The resulting biosensor demonstrated great electrocatalytic activity, good stability and fast response to MA. At the NAD + /MWCNTs-modified SPCE, the catalytic currents are linearly proportional to the concentrations of MA in the wide range from 10 nM to 140 nM with a limit of detection down to 5 nM (S/N = 3), and the biosensor exhibited a sensitivity of 18.3 μA/mM. We measured the interference effect on the MA analysis and the results demonstrated its imperviousness to the effects of haemoglobin, bilirubin and serum albumin. Measurement of mevalonic acid (MA) is of great interest in health monitoring as it is the first intermediate of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) and appears to be a good indicator of HMGR activity. HMGR plays a crucial role in regulating sterol biosynthesis. Its activity is shown as the rate-limiting step in the mevalonate pathway in the isoprenoid metabolism pathway 1,2 and thus is a prime target for the drug development. Biofluid levels of MA can be used as a biomarker for many diseases. For example, the reduction of plasma concentrations and urinary excretion of MA levels is an indirect measure of decreased cholesterol levels. For treatment of hypercholesterolemia, the statin class of drugs are used to block HMGR activity resulting in inhibition of MA synthesis.3,4 Moreover, cholesterol biosynthesis deficiencies cover a heterogeneous group of disorders. Mevalonate kinase is an enzyme located proximally in the pathway of cholesterol and nonsterol isoprene biosynthesis. Its allelic defects can cause hyperimmunoglobulinemia D syndrome (HIDS) and Mevalonic aciduria (MVA). The diagnosis of these diseases can be performed by determining MA concentration in urine followed by enzyme activity determination or mevalonate-5-phosphate (MVAP) detection by isotope dilution UPLC-MS/MS.5 Interestingly, monitoring MA levels in biofluids allows a better understanding of the drug pharmacodynamics, especially with respect to response to statin therapy due to any interindividual variations, and may allow for improvements in patient management and treatment regimes. 6 Furthermore, inhibition of HMGR may be useful for...

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Section: Electrochemical Properties Of Nadmentioning

confidence: 99%

Section: Electrochemical Properties Of Nadmentioning

confidence: 99%

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