A novel near-infrared fluorescent light-up probe for tumor imaging and drug-induced liver injury detection

Abstract

A novel light-up NIR fluorescence probe was developed and used for tumor and drug-induced liver injury imaging in vivo.

Encapsulation and Storage of Therapeutic Fibrin-Homing Peptides using Conducting Polymer Nanoparticles for Programmed Release by Electrical Stimulation

“…Analyses of the interaction between PEDOT NPs and biological fluids indicates that the protein corona is mainly governed by electrostatic forces between the NP surface and the protein charge, which is completely dependent on the solution pH. Although the therapeutic applications of CREKA are not within the scope of this work because they have been extensively demonstrated, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]28,29…”

“…In this work we are not focused on the therapeutic applications of CREKA, which have been extensively discussed, − ,, but on developing an electroactive bioplatform for storage and on-demand peptide release by electrical stimulation. For this purpose, PEDOT NPs obtained by emulsion polymerization were loaded in situ with CREKA for electro-stimulated release.…”

“…The fibrin-homing pentapeptide Cys-Arg-Glu-Lys-Ala (CREKA; Scheme ) interacts with fibrin clots and possesses favorable targeting ability to fibrin–fibronectin complexes in animal models of neoplasia, atherosclerosis, and myocardial ischemica-reperfusion. − Since this peptide was discovered by in vivo phage display technique, it has been extensively utilized for the image diagnosis of tumors − and to inhibit tumor cell migration and invasion. , The fibrinogen released from tumor vessels is transformed into fibrin fibers by thrombin, which cleaves and removes fibrinopeptides. CREKA affects this process, catalyzing a very fast aggregation process, which results in a stepwise linear growth of fibrin meshes and particles …”

Aggregation propensity of therapeutic fibrin-homing pentapeptides: insights from experiments and molecular dynamics simulations

“…12 The homing ability of CREKA (and derivatives) has been exploited as the means to direct nanostructures towards targeting specific cell lines and to enhance the nanoconstructs selectively to specific targets, that have been demonstrated both in vitro and in vivo. 15,16 Most studies were focused on probing and imaging tumours sites in sick organisms, maximizing the accumulation of nano-constructs in the targeted locations. [17][18][19] However, its use as part of cytotoxic constructs, that were built to kill specific cell lines, revealed that in most cases the destruction of tumours is preceded by the self-assembly of those nanostructures on target.…”

NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis

“…In recent years, the significant benefits of molecular imaging have been demonstrated in targeted surgery with preoperative molecular diagnostic screening and fluorescence image-guided surgery (Reinsmith et al, 2013; Iglesias et al, 2014; Walton et al, 2017; Li et al, 2019; Zeng et al, 2019). NIR fluorescence imaging can provide physiological and pathological information at the cellular and tissue levels that can be applied in biology and medicine to diagnose or treat clinical diseases (Ntziachristos et al, 2005; Pansare et al, 2012; Hong et al, 2015; Lin et al, 2019).…”