2017
DOI: 10.17957/ijab/15.0414
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A Novel Neutral Endoglucanase from Streptomyces sp. H31 with Wide-pH-range Stability

Abstract: An endoglucanase-producing strain Streptomyces sp. H31 was isolated from mangrove soil in Shenzhen, P.R.China, and the endoglucanase gene egH31 was cloned. The open reading frame (ORF) of this gene is 762bp and it encodes 253 amino acid residues, which showed the highest similarity (84%) with the endoglucanase from Streptomyces davawensis. The endoglucanase gene egH31 was successfully expressed in E. coli. We purified the recombinant EgH31 to homogeneity by affinity chromatography. Molecular mass of the recomb… Show more

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