A novel NK-lysin in hybrid crucian carp can exhibit cytotoxic activity in fish cells and confer protection against Aeromonas hydrophila infection in comparison with Carassius cuvieri and Carassius auratus red var

Luo, Sheng‐Wei; Luo, Zidong; Fan, Lanfen; Luo, Kun; Mao, Zhuangwen; Wu, Chang; Hu, Fangzhou; Wang, Shi; Wen, Ming

doi:10.1016/j.fsi.2021.06.015

Cited by 16 publications

(3 citation statements)

References 85 publications

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“…Crucian carp is an omnivore and one of the most important economic freshwater fish in China. It is abundant in rivers, lakes, and reservoirs throughout the country ( Li et al, 2018 ; Luo et al, 2021 ). Crucian carp is chosen as a model fish for this study due to its palatability, high nutritional quality, and fast growth rate ( Cui et al, 2020 ; Wu, 2020 ; Du et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Crucian carp is chosen as a model fish for this study due to its palatability, high nutritional quality, and fast growth rate ( Cui et al, 2020 ; Wu, 2020 ; Du et al, 2021 ). As is well known, environmental change can suppress fish immunity, leading fish to be infected by pathogens ( Luo et al, 2021 ). In addition, environmental fluctuations that induce physiological stress were demonstrated to affect the survival, growth, physiological, and immunological functions of aquatic animals ( Pai-Po et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

Liu

Xu³

et al. 2022

Front. Microbiol.

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Temperature changes have a great impact on fish feeding, intestinal microorganisms, metabolism, and immune function. Therefore, it is necessary to develop effective methods to enhance the survival rates and growth of fish under water temperature changes. Lactic acid bacteria (LAB) are promising immunostimulatory feed additive, as demonstrated by their beneficial effects in several fish species. This study investigated the short-term effects of dietary LAB on intestinal microbiota composition and immune responses of crucian carp (Carassius auratus) when water temperature decreased from 30 ± 1°C to 18 ± 1°C. Lactococcus (L.) lactis 1,209 and L. lactis 1,242 with potential probiotics isolated from the intestine of Qinghai naked carp (Gymnocypris przewalskii) were selected as feed additives for the crucian carp feeding experiment. A total of 225 commercially available healthy crucian carp (250 ± 10 g) of similar age were kept in 30°C water for a week and then immediately transferred to 18 ± 1°C water, assigned to three dietary treatments for a 16-day feeding trial randomly: (1) HC, diets without additives (the control group); (2) HT, diets with 106 CFU/ml L. lactis 1,209; and (3) HL, with 106 CFU/ml L. lactis 1,242. Each group was set up with 3 replicates and each with 25 fish. The results showed that the mortality rate of crucian carp in HC, HT, and HL group was 50, 27, and 33%, respectively. High-throughput sequencing results displayed that the composition of the intestinal microorganism varied dynamically in response to different treatments and water temperature decrease. Among them, compared with the HC group, a higher abundance of Firmicutes and Proteobacteria, and a lower of Actinobacteria appeared in HT and HL. The cytokines heat shock protein 70 (HSP-70) in crucian carp intestinal tract significantly decreased when water temperature decreased (p < 0.05).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

Liu

Xu³

et al. 2022

Front. Microbiol.

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“…Among the potential fish AMPs, NK-lysin-derived peptides have shown direct or indirect antibacterial, antiviral, and antiparasitic activities ( 15 , 16 , 19 , 33 , 40 , 120 – 123 ). However, only a few studies have evaluated the immunomodulatory activity of fish NK-lysin in vivo ( 16 , 27 , 33 , 124 ). We are unaware of any other reports directly showing the effects of NK-lysin over cytokines from the adaptive immune response.…”

Section: Discussionmentioning

confidence: 99%

New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides

Ortega,

Carrera,

Muñoz-Flores

et al. 2024

Front. Immunol.

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NK-lysin is a potent antimicrobial peptide (AMP) with antimicrobial activity against bacteria, fungi, viruses, and parasites. NK-lysin is a type of granulysin, a member of the saposin-like proteins family first isolated from a pig’s small intestine. In previous work, for the first time, we identified four variants of nk-lysin from Atlantic salmon (Salmo salar) using EST sequences. In the present study, we reported and characterized two additional transcripts of NK-lysin from S. salar. Besides, we evaluated the tissue distribution of three NK-lysins from S. salar and assessed the antimicrobial, hemolytic, and immunomodulatory activities and signaling pathways of three NK-lysin-derived peptides. The synthetic peptides displayed antimicrobial activity against Piscirickettsia salmonis (LF-89) and Flavobacterium psychrophilum. These peptides induced the expression of immune genes related to innate and adaptive immune responses in vitro and in vivo. The immunomodulatory activity of the peptides involves the mitogen-activated protein kinases-mediated signaling pathway, including p38, extracellular signal-regulated kinase 1/2, and/or c-Jun N-terminal kinases. Besides, the peptides modulated the immune response induced by pathogen-associated molecular patterns (PAMPs). Our findings show that NK-lysin could be a highly effective immunostimulant or vaccine adjuvant for use in fish aquaculture.

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Comparative analyses of immune gene profiles and antioxidant capabilities in the midgut and liver of three species of grass carps (Ctenopharyngodon idella) following gut infection with Aeromonas hydrophila

Luo,

Xu,

et al. 2024

Aquacult Int

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A novel NK-lysin in hybrid crucian carp can exhibit cytotoxic activity in fish cells and confer protection against Aeromonas hydrophila infection in comparison with Carassius cuvieri and Carassius auratus red var

Cited by 16 publications

References 85 publications

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides

Comparative analyses of immune gene profiles and antioxidant capabilities in the midgut and liver of three species of grass carps (Ctenopharyngodon idella) following gut infection with Aeromonas hydrophila

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