A Novel Nonhuman Primate Model for Influenza Transmission

Moncla, Louise H.; Ross, Ted M.; Dinis, Jorge M.; Weinfurter, Jason T.; Mortimer, Tatum D; Schultz-Darken, Nancy; Brunner, Kevin; Capuano, Saverio; Boettcher, Carissa; Post, Jennifer; Johnson, Michael; Bloom, Chalise E.; Weiler, Andrea M.; Friedrich, Thomas C.

doi:10.1371/journal.pone.0078750

Cited by 47 publications

(50 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Host species-specific selection on other viral nucleoproteins has been reported, such as that on influenza A virus nucleoprotein, which is restricted by the myxovirus resistance protein A (MxA) differentially in birds, humans, and nonhuman primates (32,33). Polymorphisms in host-specific restriction factors may be one possible mechanism for the diversity we observed in ORF 7 in our study.…”

Section: Discussionmentioning

confidence: 48%

Within-Host Evolution of Simian Arteriviruses in Crab-Eating Macaques

Moncla

Weiler²,

Barry³

et al. 2017

J Virol

Self Cite

View full text Add to dashboard Cite

Simian arteriviruses are a diverse clade of viruses infecting captive and wild nonhuman primates. We recently reported that Kibale red colobus virus 1 (KRCV-1) causes a mild and self-limiting disease in experimentally infected crabeating macaques, while simian hemorrhagic fever virus (SHFV) causes lethal viral hemorrhagic fever. Here we characterize how these viruses evolved during replication in cell culture and in experimentally infected macaques. During passage in cell culture, 68 substitutions that were localized in open reading frames (ORFs) likely associated with host cell entry and exit became fixed in the KRCV-1 genome. However, we did not detect any strong signatures of selection during replication in macaques. We uncovered patterns of evolution that were distinct from those observed in surveys of wild red colobus monkeys, suggesting that these species may exert different adaptive challenges for KRCV-1. During SHFV infection, we detected signatures of selection on ORF 5a and on a small subset of sites in the genome. Overall, our data suggest that patterns of evolution differ markedly among simian arteriviruses and among host species.IMPORTANCE Certain RNA viruses can cross species barriers and cause disease in new hosts. Simian arteriviruses are a diverse group of related viruses that infect captive and wild nonhuman primates, with associated disease severity ranging from apparently asymptomatic infections to severe, viral hemorrhagic fevers. We infected nonhuman primate cell cultures and then crab-eating macaques with either simian hemorrhagic fever virus (SHFV) or Kibale red colobus virus 1 (KRCV-1) and assessed within-host viral evolution. We found that KRCV-1 quickly acquired a large number of substitutions in its genome during replication in cell culture but that evolution in macaques was limited. In contrast, we detected selection focused on SHFV ORFs 5a and 5, which encode putative membrane proteins. These patterns suggest that in addition to diverse pathogenic phenotypes, these viruses may also exhibit distinct patterns of within-host evolution both in vitro and in vivo.KEYWORDS evolution, simian arterivirus, simian hemorrhagic fever virus, virology, within-host diversity I n 1964, acute outbreaks of a highly lethal viral hemorrhagic fever caused by a previously unknown agent swept through captive nonhuman primate research facilities in the former Soviet Union and the United States (1-4). Although the origin and

show abstract

Section: Discussionmentioning

confidence: 48%

Within-Host Evolution of Simian Arteriviruses in Crab-Eating Macaques

Moncla

Weiler²,

Barry³

et al. 2017

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…[120][121][122][123][124][125] Studies on animal models of influenza infection in pregnancy are limited. Here, we focus on studies in mice and nonhuman primate (cynomolgus macaque).…”

Section: Animal Studiesmentioning

confidence: 99%

Influenza, Immune System, and Pregnancy

2014

View full text Add to dashboard Cite

Influenza is a major health problem worldwide. Both seasonal influenza and pandemics take a major toll on the health and economy of our country. The present review focuses on the virology and complex immunology of this RNA virus in general and in relation to pregnancy. The goal is to attempt to explain the increased morbidity and mortality seen in infection during pregnancy. We discuss elements of innate and adaptive immunity as well as placental cellular responses to infection. In addition, we delineate findings in animal models as well as human disease. Increased knowledge of maternal and fetal immunologic responses to influenza is needed. However, enhanced understanding of nonimmune, pregnancy-specific factors influencing direct interaction of the virus with host cells is also important for the development of more effective prevention and treatment options in the future.

show abstract

“…7,8 Other species that may be used to study transmission include (outbred) guinea pigs 7,13 and, as recently identified, the common marmoset. 14 The animal models mentioned in the paragraph above each have their advantages and limitations. It has proven difficult to correlate markers Q5 of cellular immunity with pathogenesis and transmission in these animal species.…”

mentioning

confidence: 99%

Pathogenesis of Infection with 2009 Pandemic H1N1 Influenza Virus in Isogenic Guinea Pigs after Intranasal or Intratracheal Inoculation

Wiersma

Trierum

Amerongen

et al. 2015

The American Journal of Pathology

View full text Add to dashboard Cite

To elucidate the pathogenesis and transmission of influenza virus, the ferret model is typically used. To investigate protective immune responses, the use of inbred mouse strains has proven invaluable. Here, we describe a study with isogenic guinea pigs, which would uniquely combine the advantages of the mouse and ferret models for influenza virus infection. Strain 2 isogenic guinea pigs were inoculated with H1N1pdm09 influenza virus A/Netherlands/602/09 by the intranasal or intratracheal route. Viral replication kinetics were assessed by determining virus titers in nasal swabs and respiratory tissues, which were also used to assess histopathologic changes and the number of infected cells. In all guinea pigs, virus titers peaked in nasal secretions at day 2 after inoculation. Intranasal inoculation resulted in higher virus excretion via the nose and higher virus titers in the nasal turbinates than intratracheal inoculation. After intranasal inoculation, infectious virus was recovered only from nasal epithelium; after intratracheal inoculation, it was recovered also from trachea, lung, and cerebrum. Histopathologic changes corresponded with virus antigen distribution, being largely limited to nasal epithelium for intranasally infected guinea pigs and more widespread in the respiratory tract for intratracheally infected guinea pigs. In summary, isogenic guinea pigs show promise as a model to investigate the role of humoral and cell-mediated immunities to influenza and their effect on virus transmission.

show abstract

A Novel Nonhuman Primate Model for Influenza Transmission

Cited by 47 publications

References 42 publications

Within-Host Evolution of Simian Arteriviruses in Crab-Eating Macaques

Within-Host Evolution of Simian Arteriviruses in Crab-Eating Macaques

Influenza, Immune System, and Pregnancy

Pathogenesis of Infection with 2009 Pandemic H1N1 Influenza Virus in Isogenic Guinea Pigs after Intranasal or Intratracheal Inoculation

Contact Info

Product

Resources

About