A Novel Pair of Terminal Protein and Telomere-Associated Protein for Replication of the Linear Chromosome of<i>Streptomyces griseus</i>IFO13350

Suzuki, Hirokazu; Marushima, Kazuya; Ohnishi, Yasuo; Horinouchi, Sueharu

doi:10.1271/bbb.80454

Cited by 11 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), which might have been generated by recombination. The truncated tap‐tpg homologs ( SGR6987‐6986 ) were also found on the chromosome of S. griseus (Suzuki et al ., ). The latter chromosome contains a novel type of gene pair ( gtpB‐gtpA ) for end patching of the atypical telomere sequence.…”

Section: Discussionmentioning

confidence: 97%

“…The latter chromosome contains a novel type of gene pair ( gtpB‐gtpA ) for end patching of the atypical telomere sequence. Thus, the deficiency of tap‐tpg is rescued in different ways in two strains: by introducing linear plasmids, pSLA2‐L and pSLA2‐M, containing an intact tap‐tpg pair in S. rochei 7434AN4 and by acquiring a novel set of the gtpB‐gtpA genes and the atypical telomeres possibly from a certain linear plasmid in S. griseus (Suzuki et al ., ). The fact that another atypical telomere sequence of S. griseus strain 2247 was totally different from that of strain (Goshi et al ., ) supports the latter idea.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

The tap‐tpg gene pair on the linear plasmid functions to maintain a linear topology of the chromosome in Streptomyces rochei

Nindita

Cao

Yang

et al. 2015

Molecular Microbiology

View full text Add to dashboard Cite

Streptomyces rochei 7434AN4 carries three linear plasmids, pSLA2-L (211 kb), pSLA2-M (113 kb) and pSLA2-S (18 kb), their complete nucleotide sequences having been determined. Restriction and sequencing analysis revealed that the telomere sequences at both ends of the linear chromosome are identical to each other, are 98.5% identical to the right end sequences of pSLA2-L and pSLA2-M up to 3.1 kb from the ends and have homology to those of typical Streptomyces species. Mutant 2-39, which lost all the three linear plasmids, was found to carry a circularized chromosome. Sequence comparison of the fusion junction and both deletion ends revealed that chromosomal circularization occurred by terminal deletions followed by nonhomologous recombination. Curing of pSLA2-L from strain 51252, which carries only pSLA2-L, also resulted in terminal deletions in newly obtained mutants. The tap-tpg gene pair, which encodes a telomere-associated protein and a terminal protein for end patching, is located on pSLA2-L and pSLA2-M but has not hitherto been found on the chromosome. These results led us to the idea that the tap-tpg of pSLA2-L or pSLA2-M functions to maintain a linear chromosome in strain 7434AN4. This hypothesis was finally confirmed by complementation and curing experiments of the tap-tpg of pSLA2-M.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

The tap‐tpg gene pair on the linear plasmid functions to maintain a linear topology of the chromosome in Streptomyces rochei

Nindita

Cao

Yang

et al. 2015

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…It was presumably a pseudogene. Subsequently, more putative tpg pseudogenes have been found in the linear plasmids and chromosomes in many Streptomyces species , for example, S. avermitilis [10], S. ambofaciens [11], S. griseus [12], [13], and S. violaceoruber [14].…”

Section: Introductionmentioning

confidence: 99%

Mutational Analysis of the Terminal Protein Tpg of Streptomyces Chromosomes: Identification of the Deoxynucleotidylation Site

et al. 2013

View full text Add to dashboard Cite

The linear chromosomes and linear plasmids of Streptomyces are capped by terminal proteins (TPs) covalently bound to the 5′ ends of the DNA. The TPs serve as primers for DNA synthesis that patches in the single-stranded gaps at the telomeres resulting from the bi-directional replication (‘end patching’). Typical Streptomyces TPs, designated Tpgs, are conserved in sequence and size (about 185 amino acids), and contain a predicted helix-turn-helix domain and a functional nuclear localization signal. The Tpg-encoding gene (tpg) is often accompanied by an upstream gene tap that encodes an essential telomere-associating protein. Five lone tpg variants (not accompanied by tap) from various Streptomyces species were tested, and three were found to be pseudogenes. The lone tpg variant on the SLP2 plasmid, although functional, still requires the presence of tap on the chromosome for end patching. Using a combination of in vitro deoxynucleotidylation, physical localization, and genetic analysis, we identified the threonine at position 114 (T114) in Tpg of Streptomyces lividans chromosome as the deoxynucleotidylated site. Interestingly, T114 could be substituted by a serine without destroying the priming activity of Tpg in vitro and in vivo. Such T114S substitution is seen in and a number of pseudogenes as well as functional Tpgs. T114 lies in a predicted coil flanked by two short helixes in a highly hydrophilic region. The location and structural arrangement of the deoxynucleotidylated site in Tpg is similar to those in the TPs of phage ø 29 and adenoviruses. However, these TPs are distinct in their sequences and sizes, indicating that they have evolved independently during evolution. Using naturally occurring and artificially created tpg variants, we further identified several amino acid residues in the N-terminus and the helix-turn-helix domain that were important for functionality.

show abstract

“…4 genes, coding for a putative TP and a presumed Tap-helicase protein, respectively (59,60). The linear chromosome of Streptomyces griseus IFO13350 also has an unusual telomere-associated protein, which has a DnaB-like helicase C-terminal domain (54). The linear plasmid SCP1 of Streptomyces coelicolor A3(2) requires the SCP1.127 gene, coding for a unique TP, and SCP1.125 for replication of its telomeres (24,52).…”

mentioning

confidence: 99%

A Novel Replicative Enzyme Encoded by the LinearArthrobacterPlasmid pAL1

Kolkenbrock

Naumann²,

Hippler³

et al. 2010

J Bacteriol

View full text Add to dashboard Cite

The soil bacterium Arthrobacter nitroguajacolicus Rü61a contains the linear plasmid pAL1, which codes for the degradation of 2-methylquinoline. Like other linear replicons of actinomycetes, pAL1 is characterized by short terminal inverted-repeat sequences and terminal proteins (TP pAL1 ) covalently attached to its 5 ends. TP pAL1 , encoded by the pAL1.102 gene, interacts in vivo with the protein encoded by pAL1.101. Bioinformatic analysis of the pAL1.101 protein, which comprises 1,707 amino acids, suggested putative zinc finger and topoisomerase-primase domains and part of a superfamily 2 helicase domain in its N-terminal and central regions, respectively. Sequence motifs characteristic of the polymerization domain of family B DNA polymerases are partially conserved in a C-terminal segment. The purified recombinant protein catalyzed the deoxycytidylation of TP pAL1 in the presence of single-stranded DNA templates comprising the 3-terminal sequence (5-GCAGG-3), which in pAL1 forms the terminal inverted repeat, but also at templates with 5-(G/T)CA(GG/GC/CG)-3 ends. Enzyme assays suggested that the protein exhibits DNA topoisomerase, DNA helicase, and DNA-and protein-primed DNA polymerase activities. The pAL1.101 protein, therefore, may act as a replicase of pAL1.Linear plasmids have been identified in higher plants, fungi, and many bacteria. Most of these linear genetic elements are characterized by terminal inverted-repeat sequences and terminal proteins (TPs) covalently attached to their 5Ј ends. The presence of TPs is a consequence of their mode of DNA replication, which in linear plasmids of plants, yeasts, and fungi is initiated at the termini by using the TP as a primer and proceeds by strand displacement. The DNA polymerases encoded by these linear elements are of the viral B type, related to those of contemporary adenoviruses and Bacillus phages (29). The mechanism of protein-primed DNA replication has been studied in detail, especially for the model of Bacillus subtilis phage 29, which uses a monomeric B-family DNA polymerase for both the TP-primed initiation reaction and DNA elongation, resulting in continuous, full-length replication of both strands of the 29 genome (5,6,7,26,42,43,44). In contrast to the linear plasmids of eukaryotes, linear chromosomes and plasmids of Streptomyces spp. replicate bidirectionally from an internal origin (9). This replication mechanism encounters the problem that discontinuous lagging-strand synthesis from RNA-primed Okazaki fragments leaves recessed 5Ј ends at both telomeres when the distal RNA primers are removed. In the Streptomyces linear replicons, the TP serves as a primer for filling in these single-stranded gaps (2, 58). Both the TP and a telomere-associated protein (Tap), which is presumed to recruit the TP and position it at the telomere, are necessary for the propagation of Streptomyces replicons in their linear forms (3).The TP and the Tap protein are highly conserved among many Streptomyces species. On the other hand, several studies have suggested a conside...

show abstract

A Novel Pair of Terminal Protein and Telomere-Associated Protein for Replication of the Linear Chromosome ofStreptomyces griseusIFO13350

Cited by 11 publications

References 14 publications

The tap‐tpg gene pair on the linear plasmid functions to maintain a linear topology of the chromosome in Streptomyces rochei

The tap‐tpg gene pair on the linear plasmid functions to maintain a linear topology of the chromosome in Streptomyces rochei

Mutational Analysis of the Terminal Protein Tpg of Streptomyces Chromosomes: Identification of the Deoxynucleotidylation Site

A Novel Replicative Enzyme Encoded by the LinearArthrobacterPlasmid pAL1

Contact Info

Product

Resources

About