A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

Abstract

After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H 2 O 2 -induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive.

Changes in polysaccharides structure and bioactivity during Mesona chinensis Benth storage

“…The bulk structure of AMP is more irregular compared to that of FMP. This suggests that the inter- and intramolecular aggregation caused by hydroxyl group interactions on the polysaccharide chains may have occurred as a disordered spontaneous self-assembly process during the storage of the raw material ( Lin et al, 2020 ). These results are consistent with the results of Section 3.2 (Molecular weight increase after raw material storage).…”

A Natural Polymer Captor for Immobilizing Polysulfide/Polyselenide in Working Li–SeS2 Batteries

“…As for the FT-IR spectra of NPP (Fig. S2), the distinct peaks at 3419 and 2934 cm −1 belong to the stretching vibration of O–H and C–H bonds, respectively [ 34 ]. The peaks at 1728 and 1608 cm −1 are present due to the stretching vibrations of C=O bonds in ester and carboxyl, respectively [ 29 , 35 ].…”

“…The peaks located between 1200 and 1000 cm −1 correspond to the bending vibration of C–O–C glycosidic bonds in polysaccharide [ 37 , 38 ]. Note that the weak absorption peaks in the low frequency region of 900–800 cm −1 are probably related to α-glycosidic bonds, which implies the existence of α -glycosidic bonds in NPP [ 34 ]. These results demonstrate that the NPP certainly is abundant in oxygen-containing groups, which could afford a powerful adsorption toward Li 2 S n /Li 2 Se n and thus retard the shuttle effect.…”

Research advances in bioactive components and health benefits of jujube (Ziziphus jujuba Mill.) fruit

“…HPLC analysis indicated that JCS-1 and JCS-2 were composed mainly of galacturonic acid (GalA) (Table 1). A recent study found that SAZMP4 (a novel antioxidant pectin isolated from jujube) was composed mainly of rhamnose, arabinose, xylose, mannose, and GalA through 1,4linked GalA (93.48%) (Lin et al, 2020).…”