A novel pentasaccharide from immunostimulant oligosaccharide fraction of buffalo milk

Saksena, Rina; Deepak, Desh; Khare, Anakshi; Sahai, Ragini; Tripathi, L.M.; Srivastava, V. M. L.

doi:10.1016/s0304-4165(99)00089-6

Cited by 33 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oligosaccharides are also involved in many cellular recognition processes and have numerous biological activities including immunostimulatory, anti-infl ammatory, antiviral and immunological function (89).…”

Section: Oligosaccharides With Prebiotic Propertiesmentioning

confidence: 99%

Cheese as Functional Food: The Example of Parmigiano Reggiano and Grana Padano

Summer

Formaggioni

Franceschi

et al. 2017

Food Technol. Biotechnol.

View full text Add to dashboard Cite

SummaryItalian hard cooked types of cheese, like Parmigiano Reggiano and Grana Padano, are characterised by positive nutritional qualities. In fact, they contain substances that have particular biological activities, and therefore they can be fully considered, according to the defi nition given by the European Unit, as 'functional' foods. This short review concisely describes these components and the benefi cial eff ects related to their activities. The description of the biologically active components has been organised in the following paragraphs: protein and peptides, fat and lipids, carbohydrates and prebiotics, probiotic bacteria, vitamins, mineral salts, and components of dairy products active in disease prevention. In particular, several known bioactive peptides were found in Parmigiano Reggiano cheese samples: for example, phosphopeptides, which are known for their mineral-binding capacity and vehiculation activity, peptides with immunomodulatory activity, and angiotensin-converting enzyme-inhibitory peptides with anti-hypertensive eff ects. Among lipids, the role of conjugated linoleic acid and other fatt y acids present in these cheese types was taken into consideration. The presence of oligosaccharides with prebiotic properties and probiotic bacteria was also described. Finally, particular emphasis was given to highly available calcium and its impact on bone health.

show abstract

Section: Oligosaccharides With Prebiotic Propertiesmentioning

confidence: 99%

Cheese as Functional Food: The Example of Parmigiano Reggiano and Grana Padano

Summer

Formaggioni

Franceschi

et al. 2017

Food Technol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…These fractions were pooled and lyophilized together. Due to the highly polar nature of oligosaccharide, derivatization to acetylate them was done as described earlier (14). Separation of the acetylated product (150 mg) was carried over a silica gel (50 g) by eluting with CHCl 3 and MeOH, thereby resulting in 10 fractions (50 ml each).…”

Section: Methodsmentioning

confidence: 99%

Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

Srivastava

Singh

Deepak

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD), 1 H and 13 C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS against S. aureus and its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance in S. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial.

show abstract

“…Recently, various carbohydrates obtained from natural products have come into the light as important bioactive compounds. Oligosaccharides played an essential role in many molecular processes impacting eukaryotic biology and diseases and displayed varied biological activities (Saksena et al 1999;Bland et al 2004). Moreover, much in vivo and in vitro evidence has demonstrated that natural oligosaccharides displayed immunomodulating function by stimulating both cellular and humoral immunoresponse (Wang et al 2001;Nergard et al 2006).…”

Section: Immunological Activities Of the Wgos And Its Fractionationsmentioning

confidence: 99%

Structural characterization and immunological activities of the water-soluble oligosaccharides isolated from the Panax ginseng roots

Wan

Jiao

Yang

et al. 2011

Planta

View full text Add to dashboard Cite

Water-soluble ginseng oligosaccharides (designated as WGOS) with a degree of polymerization ranging from 2 to 10 were obtained from warm-water extract of Panax ginseng roots, and fractionated into five purified fractions (i.e., WGOS-0, WGOS-1, WGOS-2, WGOS-3, and WGOS-4) by gel-filtration chromatography. In order to ascertain the monosaccharide residues in the WGOS, a technique that combines acid hydrolysis and high-performance liquid chromatography was employed. It was found that only glucose residues were present in the WGOS. Fourier transform infrared spectroscopy and electrospray ionization tandem mass spectrometry provided the sequence, linkage, and configuration information. It is noteworthy that α-Glcp-(1 → 6)-α-Glcp, α-Glcp-(1 → 6)-α-Glcp-(1 → 4)-α-Glcp, α-Glcp-(1 → 6)-α-Glcp-(1 → 6)-α-Glcp-(1 → 4)-α-Glcp, and other six malto-oligosaccharides (i.e., maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, and maltodecaose) were detected in ginseng. Preliminary immunological tests in vitro indicated that WGOS were potent B and T-cell stimulators and WGOS-1 has the highest immunostimulating effect on lymphocyte proliferation among those purified fractions. It is hoped that the WGOS will be developed into functional food or medicine.

show abstract

A novel pentasaccharide from immunostimulant oligosaccharide fraction of buffalo milk

Cited by 33 publications

References 43 publications

Cheese as Functional Food: The Example of Parmigiano Reggiano and Grana Padano

Cheese as Functional Food: The Example of Parmigiano Reggiano and Grana Padano

Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

Structural characterization and immunological activities of the water-soluble oligosaccharides isolated from the Panax ginseng roots

Contact Info

Product

Resources

About