A Novel Phage Infecting the Marine Photoheterotrophic Bacterium Citromicrobium bathyomarinum

Ma, Ruijie; Shao, Shuai; Wei, Shuzhen; Ye, Junlei; Yang, Yue; Jiao, Nianzhi

doi:10.3390/v14030512

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Every 10 min, subsamples were collected, and the viral abundance was detected using the double-layer agar method. The burst size was calculated as the ratio between the number of virions at the growth plateau and the initial number of infected host cells [54].…”

Section: Methodsmentioning

confidence: 99%

Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family

Wei,

Li,

et al. 2024

Preprint

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Background Vibrio parahaemolyticus has been a leading cause of foodborne disease outbreaks and infectious diarrhea cases in coastal areas, antibiotic resistance has increased significantly due to widespread antibiotic abuse, bacteriophages (phages) are viruses that specifically infect bacteria, it is necessary to isolate and characterize new phages to broaden our understanding of the ecology, evolution, and diversity of both phages and their bacterial hosts further. Results A novel vibriophage vB_VpaM_XM1 (XM1) was described in the present study. The morphological analysis revealed that phage XM1 had Myoviridae-like morphology, with an oblate icosahedral head and a long contractile tail. The genome size of XM1 is 46,056 bp, with a G + C content of 42.51%, encoding 69 open reading frames (ORFs). Moreover, XM1 showed a narrow host range only lysing Vibrio xuii LMG 21346 (T) JL2919, Vibrio parahaemolyticus 1.1997, and Vibrio parahaemolyticus MCCC 1H00029 among the tested bacteria. One-step growth curves showed that XM1 has a 40-minute latent period and 264 plaque-forming units (PFU)/cell burst size. In addition, XM1 exhibited broad pH, thermal, and salinity stability, as well as strong lytic activity, even at a multiplicity of infection (MOI) of 0.001. Multiple genome comparisons and phylogenetic analyses showed that phage XM1 is grouped in a clade with three other phages, including Vibrio phages Rostov 7, X29, and phi 2, and is distinct from all known viral families that have ratified by the standard genomic analysis of the International Committee on Taxonomy of Viruses (ICTV). Conclusions Therefore, the above four phages might represent a new viral family, tentatively named Weiviridae. The broad physiological adaptability of phage XM1 and its high lytic activity and host specificity indicated that this novel phage is a good candidate for being used as a therapeutic bioagent against infections caused by certain Vibrio parahaemolyticus strains.

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Section: Methodsmentioning

confidence: 99%

Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family

Wei,

Li,

et al. 2024

Preprint

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“…Here, we established a systematic CbK‐like collection with a total of 30 members (Table 1), including the CbK‐like phage vB_CbaS‐RXM that we recently reported (Ma et al, 2022) and six CbK‐like sequences (including prophage genomes) that we identified from different proteobacterial genomes. To predict the capacity of these phages for lysogeny, the phage recombinase and attP – attB pairing were systematically analysed, and both culture‐dependent and culture‐independent lysogeny tests were conducted.…”

Section: Introductionmentioning

confidence: 99%

Diversity, evolution and life strategies of CbK‐like phages

Chen

et al. 2023

Environmental Microbiology

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Caulobacter phage CbK has been extensively studied as a model system in virology and bacteriology. Lysogeny-related genes have been found in each CbK-like isolate, suggesting a life strategy of both lytic and lysogenic cycles. However, whether CbK-related phages can enter lysogeny is still undetermined. This study identified new CbK-like sequences and expanded the collection of CbK-related phages. A common ancestry with a temperate lifestyle was predicted for the group, however, which subsequently evolved into two clades of different genome sizes and host associations. Through the examination of phage recombinase genes, alignment of attachment sites on the phage and bacterial genomes (attP-attB pairing), and the experimental validation, different lifestyles were found among the different members. A majority of clade II members retain a lysogenic lifestyle, whereas all clade I members have evolved into an obligate lytic lifestyle via a loss of the gene encoding Cre-like recombinase and the coupled attP fragment. We postulated that the loss of lysogeny may be a by-product of the increase in phage genome size, and vice versa. Clade I is likely to overcome the costs through maintaining more auxiliary metabolic genes (AMGs), particularly for those involved in protein metabolism, to strengthen host takeover and further benefit virion production.

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Isolation, characterization, and application of a novel Pseudomonas fluorescens phage vB_PF_Y1-MI in contaminated milk

Xuan,

Liu,

Wang

et al. 2024

Mol Genet Genomics

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A Novel Phage Infecting the Marine Photoheterotrophic Bacterium Citromicrobium bathyomarinum

Cited by 5 publications

References 39 publications

Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family

Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family

Diversity, evolution and life strategies of CbK‐like phages

Isolation, characterization, and application of a novel Pseudomonas fluorescens phage vB_PF_Y1-MI in contaminated milk

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