A novel platform for enhanced biosensing based on the synergy effects of electrospun polymer nanofibers and graphene oxides

Abstract

A novel biosensing platform was developed by combining the advantages of electrospun poly(vinyl alcohol) (PVA)/chitosan nanofibers and graphene oxides (GO). Glucose oxidase (GOD) was employed as a model enzyme. By co-electrospinning the solution of PVA, chitosan, GOD and GO, the PVA/chitosan/GOD/GO nanofibers were directly modified on the platinum (Pt) electrode. The UV-vis spectra and the FTIR spectra were used to characterize the GO nanosheets. The morphologies of fabricated electrospun nanofibers were characterized by high resolution scanning electron microscopy. After a thin layer of nafion was modified on the surface of matrix, the as-prepared electrode was used to detect glucose. The electrode exhibited great advantages in high sensitivity, low detection limit and wide linear range. In the meantime, the electrode showed good stability, acceptable reproducibility, and excellent anti-interference capability for ascorbic acid, uric acid, lactose and sucrose. Moreover, the novel biosensor was successfully applied for the glucose determination in human serum samples. The mechanism of efficient biosensing of the nafion/PVA/chitosan/GOD/GO/Pt electrode was analyzed in detail and the results show that it can be due to the synergy effects of electrospun nanofibers and GO nanosheets.

Development of Biosensors from Polymer Graphene Composites

“…During the last 20 years, electronically polymers like PANi, PPy, and polythiophene have received significant attention because of their easy synthetic procedures, less time consuming, remarkable electronic, magnetic, and optical properties and their wide range of potential applications in many areas [82][83][84][85][86][87][88][89][90][91][92][93][94].…”

“…Wisitsoraat et al [93] developed graphene-PDEOT:PSS composite based on electrophoretic method. Su et al [94] demonstrated the synthesis of PVA-chitosan-GO nanocomposites by electrospinning the solution of PVA, chitosan, GOx and GO.…”

Graphene modified electrospun poly(vinyl alcohol) nanofibrous membranes for glucose oxidase immobilization

“…Many nanomaterials, such as carbon nanotubes [5,18], gold nanoparticles [19,20], and metal oxides [21,22] have been used in biosensors. More recently a new class of carbon material, graphene, which is a twodimensional sheet of carbon atoms, has attracted increasing attention with respect to its potential applications in phtoelectronic devices, supercapacitors, sensors and nanocomposites applications [6,[23][24][25][26][27][28][29][30][31][32]. Due to their planar morphology and thus larger accessible surface area, graphene may perform better than any other carbon-based materials for an electrode sensor fabrication.…”

“…The high sensitivity and good stability of such a modified electrode contributed to the construction of a practical glucose biosensor. Su et al [27] reported the synergy mechanism of electrospun PVA/chitosan nanofibers and graphene nanosheets for efficient glucose biosensing. These results indicate graphene shows significant potential for fabricating an electrochemical sensing interface.…”

One-Step Fabrication of Electrospun Photo-Cross-Linkable Polymer Nanofibers Incorporating Multiwall Carbon Nanotubes and Enzyme for Biosensing

“…21 The water stability of PVA NFs may be improved through cross-linking by exposure to glutaraldehyde solutions [22][23] or vapors. 24 but this additional posttreatment step is time-consuming and requires careful optimization to preserve enzyme conformation and activity. Some works focusing on the fabrication of electrospun NFs from enzyme/PVA blends have been already published 19,[22][23][24][25][26][27] but applications to the elaboration of enzyme electrochemical biosensors are rather scarce.…”