A novel polymer‐based genosensor for the detection and quantification of Streptococcus pneumoniae in genomic DNA sample

Abstract

The Streptococcus pneumoniae detection plays an important role in the diagnosis and monitoring of pneumococcal diseases. A genosensor based on graphite electrodes modified with polymer was developed for such detection. First, the poly(4‐aminophenol) film was electrochemically deposited on a graphite electrode. Afterward, an S. pneumoniae‐specific oligonucleotide (Strep1), isolated from conserved regions of the bacterial genome, was immobilized onto the modified electrode surface and used for the test with complementary target oligonucleotide (Strep2) or genomic DNA. The genosensor was evaluated using electrochemical techniques and atomic force microscopy. Poly(4‐aminophenol) film caused an increase in probe immobilization, monitoring the guanine oxidation peak. The detection limits obtained using differential pulse voltammetry and electrochemical spectroscopy impedance were 54 and 28 ng mL−1, respectively. The novel genosensor was efficient for the immobilization and detection of S. pneumoniae genomic DNA. POLYM. ENG. SCI., 58:1308–1314, 2018. © 2017 Society of Plastics Engineers

Recent advances in airborne pathogen detection using optical and electrochemical biosensors

“…Ethidium bromide (EB) functions as an indicator that was efficiently intercalated into the dsDNA. Notably, the current responses generated by EB in the presence of S. pneumoniae were monitored using DPV analysis [ 143 ]. Using this process, the LOD was 54 ng mL −1 , revealing lower sensitivity than the colorimetric LAMP-based method [ 8 ].…”

Recent progress and challenges on the bioassay of pathogenic bacteria

“…Thereafter, a particular sequence of S. pneumoniae was fixed on the modified electrode superficies and employed to the assay with genomic DNA or complementary tDNA (Scheme 8). The achieved detection limits using EIS (electrochemical impedance spectroscopy) and differential pulse voltammetry were 28 and 54 ng/ml, respectively 120 …”

“…For complete of discussion, Table 2 25‐206 summarized all of the discussed articles on the detection of bacterial. It is important to point out that, there is other reports in this research area …”

“…The achieved detection limits using EIS (electrochemical impedance spectroscopy) and differential pulse voltammetry were 28 and 54 ng/ml, respectively. 120 Sotillo and colleagues was developed an amperometric magneto- values of 90% specificity and 91% sensitivity were measured using the analysis of ROC curve. Also, this strategy was applied to directly determine pneumococci in clinical specimens.…”

Electrochemical Genosensors: Definition and Fields of Application

“…17 Electrochemical genosensors offer a quick tool for detection and diagnosis, based on the use of specific probe for gene of virulence, such inlA and hly gene for Listeria monocytogenes , 7,17 or oligonucleotides isolated from conserved region of the bacteria genome for Streptococcus pneumonia. 18 Infection by Streptococcus pyogenes causing damage of heart valves in human, as reported by 8 an electrochemical genosensor based on sepB gene probe can offer a rapid detection in few minutes (30 min) of the infection with a lower LOD of 0.10ng of ssG-DNA per 6μl (human throat swab sample s). Based on oligonucleotides probe that was identified from the 16s rRNA coding region of the Escherichia coli O157:H7 genome, Pandey et al 19 have coupled it with cystine flower-gold particles matrice to improve its immobilization, their genosensor exhibited a linear response with LOD of 1x10 -15 M provide a selective tool for Escherichia coli O157:H7.…”