A novel polymerase chain reaction assay for the detection and speciation of thermophilic Campylobacter spp.

Jackson, C. J.; Jones, D. M.

doi:10.1111/j.1365-2672.1996.tb03534.x

Cited by 34 publications

(44 citation statements)

References 24 publications

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“…The Bolton broth culture was inoculated onto modified charcoal cefoperazone deoxycholate agar (Oxoid, Inc.) by using a cotton-tipped swab followed by streaking. After 48 h of incubation, presumptive Campylobacter colonies (small, grey, moist, and flat spreading or droplike; five per sample) were transferred to blood agar plates and incubated for 24 h. Morphological examinations, catalase and oxidase tests, and species-specific PCR assays were performed as described previously (16,23,28). After confirmation, up to three Campylobacter isolates per sample were stored at 280uC in brain heart infusion broth with 30% glycerol in cryogenic vials.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Antimicrobial Susceptibility of Major Foodborne Pathogens in Imported Seafood

Wang

Jiang

Yang

et al. 2011

Journal of Food Protection

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Seafood is a leading commodity implicated in foodborne disease outbreaks in the United States. Seafood importation rose dramatically in the past 3 decades and now contributes to more than 80% of the total U.S. seafood supply. However, limited data are available on the microbiological safety of imported seafood. In this study, we obtained a total of 171 salmon, shrimp, and tilapia samples imported from 12 countries in three retail stores in Baton Rouge, LA. The total microbial population and the prevalence and antimicrobial susceptibilities of six major foodborne-pathogen genera (Campylobacter, Escherichia coli, Listeria, Salmonella, Shigella, and Vibrio) were determined. The aerobic plate counts (APC) for the 171 samples averaged 4.96 log CFU/g, with samples from Chile carrying the highest mean APC of 6.53 log CFU/g and fresh samples having a significantly higher mean APC than frozen ones (P < 0.0001). There were 27 samples (15.8%) with unacceptable microbiological quality (APC > 7 log CFU/g). By culture, no sample tested positive for Campylobacter coli, Shigella, or Vibrio vulnificus. Campylobacter jejuni and Salmonella enterica serovar Typhimurium were each recovered once from farm-raised tilapia from China. By PCR, 17.5 and 32.2% of the samples were positive for Salmonella and Shigella, respectively. The overall prevalence rates of other target bacteria were low, ranging from 4.1% for Listeria monocytogenes to 9.4% for E. coli. All of the Vibrio parahaemolyticus isolates recovered were from shrimp, and 63.3% showed intermediate resistance to ampicillin. Both C. jejuni isolates possessed a rare resistance to gentamicin, while 75% of L. monocytogenes isolates were resistant to nitrofurantoin. Taken together, these findings suggest potential food safety hazards associated with imported seafood and warrant further large-scale studies.

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Section: Methodsmentioning

confidence: 99%

Prevalence and Antimicrobial Susceptibility of Major Foodborne Pathogens in Imported Seafood

Wang

Jiang

Yang

et al. 2011

Journal of Food Protection

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“…The authors concluded that the repair of water mains most probably resulted in contamination and that nonchlorinated ground-water systems may be susceptible to contamination by campylobacters. Jackson et al (1996) developed a novel PCR assay for the detection and speciation of C. jejuni, C. coli and C. upsaliensis. The primers BO4263 and BO4264 (Table 6) amplify a 256-bp sequence of a novel open reading frame adjacent to and downstream of a novel C. jejuni two-component regulator gene.…”

Section: Pulsed Field Gel Electrophoresis (Pfge)mentioning

confidence: 99%

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Levin

2007

Food Biotechnology

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Campylobacter jejuni is considered to be the leading cause of enteric illness in the United States and other industrialized nations, causing mild to severe symptoms including serious infections of the extremities and permanent neurological symptoms. The organism is a cytochrome oxidase positive, microaerophilic, curved Gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry, where the intestine is colonized resulting in healthy animals as carriers. Wildlife have long been considered an infectious reservoir for campylobacters because of their close association with and contamination of surface waters. This review deals with the areas of: phenotypic characteristics of C. jejuni and related human pathogenic species of Campylobacter, their ecological distribution, virulence factors, isolation of C. jejuni from foods, serotyping of Campylobacter isolates, bacteriophage typing, molecular methods of detecting and typing campylobacters, the viable but nonculturable state of campylobacters, the coccoid form of C. jejuni and immunomagnetic capture of C. jejuni.

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“…A number of PCR assays have been described for the detection of campylobacters from food and faecal samples (1, 4, 6, 13, 14). The present study was carried to access the prevalence of C. jejuni in various food and faecal samples and to compare the efficacy of cultural and biochemical tests with PCR for detection of the organism.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

Singh¹,

Rathore²,

Singh³

et al. 2011

Braz. J. Microbiol.

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In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8, respectively, could be detected by isolation whereas 6, 3 and 10, respectively, were found positive by PCR. However, among food samples viz. beef (30), milk (35), cheese (30), only one beef sample was detected both by culture as well as PCR. Additionally, PCR was found to be more sensitive for C. jejuni detection in spiked faecal and food samples (96.1% each) as relative to culture isolation which could detect the organism in 86.7% and 80% samples, respectively. The results depicted the superior efficacy of PCR for rapid screening of samples owing to its high sensitivity, specificity and automation potential.

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A novel polymerase chain reaction assay for the detection and speciation of thermophilic Campylobacter spp.

Cited by 34 publications

References 24 publications

Prevalence and Antimicrobial Susceptibility of Major Foodborne Pathogens in Imported Seafood

Prevalence and Antimicrobial Susceptibility of Major Foodborne Pathogens in Imported Seafood

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

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