A Novel Polysaccharide from AuriculariaAuricula Alleviates Thrombosis Induced by Carrageenan in Mice

Abstract

The increasing incidence of cardiovascular diseases has created an urgent need for safe and effective antithrombotic agents. In this study, we aimed to elucidate the structural characteristics and antithrombotic activity of a novel polysaccharide isolated from Auriculariaauricula fruiting bodies. The purified polysaccharide AAP-b2 (12.02 kDa) was composed of mannose, glucuronic acid, glucose and xylose, with a molar ratio of 89.25:30.50:4.25:1.00. Methylation and NMR analyses showed that AAP-b2 primarily consisted of →2,3)-Manp-(1→, →3)-Manp-(1→, →4)-GlcAp-(1→ and Manp-(1→. A thrombus mouse model induced by carrageenan was used in this research to evaluate its antithrombotic effect. AAP-b2 significantly inhibited platelet aggregation, reduced the black tail length and prolonged the coagulation time, including activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT), exerting a good inhibitory effect on thrombosis in mice. The antithrombotic activity of AAP-b2 was found to be related to the inhibition of platelet activation by regulation of endothelial nitric oxide synthases (eNOs), endothelin-1 (ET-1), prostacyclin (PGI2) and thromboxane B2 (TXB2), along with the enhancement of anticoagulant activity by affecting antithrombin III (AT-III) and protein C (PC) pathways.

Near-Infrared Fluorescent Probe with pH- and Viscosity-Switchable Performance for the Detection of Thrombi in Live Animals and Organs

“…Carrageenan was used to induce blood viscosity change and produce blood clots in live mice. The mice had black tails due to blood clots. − The exogenous viscosity change was measured in mice via QL2 . After exogenous injection of 50%, 70%, 80%, and 90% glycerol, the fluorescence of the blood clot group in both channels gradually increased, compared to that of the control group (Figure ).…”

Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model

“…Endothelin-1 (ET-1) and TXB2 have a prothrombotic effect, while prostacyclin I2 (PGI2) has a role in anti-platelet aggregation, thus preventing thrombosis [22]. In this study, thrombosis parameters were investigated to evaluate the effects of FJH-KO on thrombus formation.…”

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“…With the increasing number of cardiovascular diseases, the demand for safe and effective drugs for the treatment and prevention of thrombosis has grown. In a study conducted by Bian et al (2022), the structural characteristics and thrombolytic (fibrinolytic) activity of a new polysaccharide isolated from the fruiting bodies of A. auricula were determined. The purified polysaccharide AAP-b2 (12.02 kDa) consisted of mannose, glucuronic acid, glucose and xylose with a molar ratio of 89.25::30.50:4.25:1.00.…”