A novel Pro197Glu substitution in acetolactate synthase (ALS) confers broad-spectrum resistance across ALS inhibitors

Liu, Weitang; Yuan, Guohui; Du, Long; Guo, Wenlei; Li, Lingxu; Bi, Yang; Wang, Jinxin

doi:10.1016/j.pestbp.2014.10.005

Cited by 34 publications

(26 citation statements)

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“…Twice-selected resistant plants derived from HN10 were used for experiments. Briefly, a single-rate screen test was performed to identify resistant plants as described in our previous study [ 27 ]. Plants that survived treatment with 22.5 g ai ha − 1 (2-fold higher than the field recommend rate) TM (75% WG; DuPont, Juxing, Shanghai, China) were selected for ALS gene sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…Water chickweed ( Myosoton aquaticum (L.)) is a diploid dicot broadleaf weed that is widespread in winter fields in China and has evolved resistance to many ALS inhibitors [ 27 ]. Previous studies have reported ALS mutations in M. aquaticum , identifying TSR as the major mechanism of herbicide resistance although coexisting NTSR was observed in many populations [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)

et al. 2018

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BackgroundWater chickweed (Myosoton aquaticum (L.)) is a dicot broadleaf weed that is widespread in winter fields in China, and has evolved serious resistance to acetolactate synthase (ALS) inhibiting herbicides.ResultsWe identified a M. aquaticum population exhibiting moderate (6.15-fold) resistance to tribenuron-methyl (TM). Target-site ALS gene sequencing revealed no known resistance mutations in these plants, and the in vitro ALS activity assays showed no differences in enzyme sensitivity between susceptible and resistant populations; however, resistance was reversed by pretreatment with the cytochrome P450 (CYP) monooxygenase inhibitor malathion. An RNA sequencing transcriptome analysis was performed to identify candidate genes involved in metabolic resistance, and the unigenes obtained by de novo transcriptome assembly were annotated across seven databases. In total, 34 differentially expressed genes selected by digital gene expression analysis were validated by quantitative real-time (qRT)-PCR. Ten consistently overexpressed contigs, including four for CYP, four for ATP-binding cassette (ABC) transporter, and two for peroxidase were further validated by qRT-PCR using additional plants from resistant and susceptible populations. Three CYP genes (with homology to CYP734A1, CYP76C1, and CYP86B1) and one ABC transporter gene (with homology to ABCC10) were highly expressed in all resistant plants.ConclusionThe mechanism of TM resistance in M. aquaticum is controlled by NTSR rather than TSR. Four genes, CYP734A1, CYP76C1, CYP86B1, and ABCC10 could play essential role in metabolic resistance to TM and justify further functional studies. To our knowledge, this is the first large-scale transcriptome analysis of genes associated with NTSR in M. aquaticum using the Illumina platform. Our data provide resource for M. aquaticum biology, and will facilitate the study of herbicide resistance mechanism at the molecular level in this species as well as in other weeds.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1451-x) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)

et al. 2018

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“…ALS inhibitor are selective for several crops and requires minimal doses for weed control, thus presenting low toxicity to mammals. However, the intense use of these herbicides has frequently led to the selection of more resistant weeds worldwide (Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Identification and Mapping of Cross-Resistance Patterns to ALS-Inhibitors in Greater Beggarticks (Bidens spp.)

et al. 2019

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Greater beggarticks (Bidens pilosa and Bidens subalternans) biotypes have been under selection pressure of ALS-inhibitors since early 90’s in Brazil. The objectives of this work were to investigate whether there are different cross-resistance patterns among ALS-inhibitors herbicides in Bidens spp. biotypes; to understand the geographic distribution of resistance patterns in grains producing regions in Brazil; and evaluate the possibility of multiple resistance to ALS-inhibitors, EPSPs inhibitor and photosystem II inhibitors. Dose-response experiments were carried out with imazethapyr, chlorimuron and diclosulam in three populations. Sensibility to others 34 populations both from Paraná State (PR) and from others Brazilian regions were also evaluated. The dose-response assay revealed cross-resistance with different patterns. One population was resistant to all three herbicides, the second population was tolerant to both imazethapyr and chlorimuron, but not to diclosulam, while a third population was resistant merely to imazethapyr. The results exhibited different cross-resistance patterns, since they can be found in other Bidens spp. populations. However, no relationship was observed between geographic areas where samples were collected and resistance patterns. Conclusively, the most frequent resistance pattern was R2 (resistance to imazethapyr, chlorimuron and diclosulam).

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“…The flixweed with Pro-197-Ser or Pro-197-Leu displayed different cross-resistance patterns to SU, TP and IMI herbicides (Deng et al 2014). A novel Pro197Glu substitution in water chickweed (Myosoton aquaticum) showed resistance to both tribenuron-methyl (SU) and imazethapyr (IMI) (Liu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

Deng

Yang

HongTao

et al. 2016

Journal of Integrative Agriculture

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Flixweed (Descurainia sophia L.) is a problematic and widespread weed in winter wheat fields and has been controlled by tribenuron-methyl for more than twenty years in China. In this study, a flixweed accession (HB25) with an Asp-376-Glu mutation in acetohydroxy acid synthase (AHAS) was identified and purified. The purified HB25 accession (pHB25) developed 758.1-fold resistance to tribenuron-methyl and exhibited obvious cross-resistance to four AHAS-inhibiting herbicides. The R/S ratios of GR 50 to herbicides of halosulfuron-methyl (SU), flumetsulam (TP), imazethapyr (IMI) and pyribenzoxim (PTB) were 346.1 15.7, 8.1 and 7.1, respectively. The reduced AHAS sensitivities to four different AHAS-inhibiting herbicides, which were caused by the Asp-376-Glu mutation, were responsible for the resistance and cross-resistance to AHAS-inhibiting herbicides. The R/S ratios of I 50 to tribenuron-methyl, halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 844.5, 532.9, 74.5, 13.3 and 5.5 respectively. The results of AHAS activity in vitro were highly correlated with that of whole-plant response experiments.

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A novel Pro197Glu substitution in acetolactate synthase (ALS) confers broad-spectrum resistance across ALS inhibitors

Cited by 34 publications

References 34 publications

Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)

Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)

Identification and Mapping of Cross-Resistance Patterns to ALS-Inhibitors in Greater Beggarticks (Bidens spp.)

Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

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