A novel probe based on phenylboronic acid functionalized carbon nanotubes for ultrasensitive carbohydrate determination in biofluids and semi-solid biotissues

Chen, Guosheng; Qiu, Junlang; Xu, Jianqiao; Fang, Xuan; Liang, Yan; Li, Shuqin; Wei, Songbo; Jiang, Ruifen; Luan, Tiangang; Zeng, Feng; Zhu, Fang; Ouyang, Gangfeng

doi:10.1039/c5sc03992d

Cited by 65 publications

(39 citation statements)

References 47 publications

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“…Given the desirable recognition capacity of OSI‐MNPs and the rapid allochroic feature of PBA‐AGO, the potential of AGO‐OSI for glycoprotein assay was then examined. We noted that the lewis acid‐like boronic acid groups were able to rapidly incorporate proteins through boronate‐affinity chemistry, including the covalent affinity (between boronic acid groups and cis‐diols unites within the glycoproteins) and the B–N interactions (between boronic acid groups and the N‐terminal of the proteins) . Thus, it is reasonable to suppose that PBA‐AGO could efficiently label target glycoproteins via boronate‐affinity chemistry, forming a OSI‐MNPs/glycoproteins/PBA‐AGO sandwich assembly.…”

Section: Resultsmentioning

confidence: 99%

Allochroic‐Graphene Oxide Linked 3D Oriented Surface Imprinting Strategy for Glycoproteins Assays

Chen

Kou

Huang

et al. 2018

Adv Funct Materials

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Affordable methodologies for screening of disease-related biomarkers are vital for early clinical diagnosis, and can potentially improve the survival rate. Herein, a cost-efficient and easy-to-use approach, called allochroic-graphene oxide linked oriented surface imprinting strategy (AGO-OSI), is imparted for rapid glycoproteins screening with naked eyes. The novel detection principle herein relies on the boronate-affinity sandwich-like assembly, in which the target glycoproteins are specifically captured by the engineered 3D oriented surface imprinting magnetic microprobe, and followed by labeling with the pH-triggered allochroic-graphene oxide. Compared with the enzyme-linked immunosorbent assay, no antibody-assisted recognition or complicated enzymatic labeling is required, endowing the AGO-OSI with the merits of low-cost, labor-saving, and user-friendly. In addition, the pH-triggered allochroic-graphene oxide provides a simple, real-time, and economical signal readout, without the sophisticated instruments requirement. Finally, it is successfully applied to rapid diagnosis of α-fetoprotein (AFP) in liver cancer event with naked eyes, further manifesting the superiority of AGO-OSI. It is believed that the cost-efficient and easy-to-use strategy proposed herein can potentially be a versatile tool for point-of-care diagnosis, especially applicable in resource-constrained regions.

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Section: Resultsmentioning

confidence: 99%

Allochroic‐Graphene Oxide Linked 3D Oriented Surface Imprinting Strategy for Glycoproteins Assays

Chen

Kou

Huang

et al. 2018

Adv Funct Materials

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“…A clear chromatogram profile ( Figure 4B)w ith as trong glucose peak was observed owing to the high selectivity and excellent tolerance of the BA-MIPsp robe.F or comparison, av ery complex chromatogram profile was observed using ar ecently reported biocompatible carbohydrate probe, due to the interference of other saccharides as well as other unwanted species from aloe leaf. [17] Furthermore, MALDI-TOF MS analysis demonstrated that glucose was captured ( Figure 4C)w hile no macromolecules were extracted, which confirmed the good biocompatibility of the BA-MIPs probe ( Figure S13). Finally,t he BA-MIPs probe was used for low-destructivea nd long-term in vivo continuous glucosemonitoringi na loe leaf ( Figure 4D).…”

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confidence: 75%

Boronate Affinity–Molecularly Imprinted Biocompatible Probe: An Alternative for Specific Glucose Monitoring

Chen

Qiu

Fang

et al. 2016

Chemistry An Asian Journal

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A biocompatible probe for specific glucose recognition is based on photoinitiated boronate affinity-molecular imprinted polymers (BA-MIPs). The unique pre-self-assembly between glucose and boronic acids creates glucose-specific memory cavities in the BA-MIPs coating. As a result, the binding constant toward glucose was enhanced by three orders of magnitude. The BA-MIPs probe was applied to glucose determination in serum and urine and implanted into plant tissues for low-destructive and long-term in vivo continuous glucose monitoring.

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“…The need for representative information regarding the actual composition and the dynamics of xenobiotics and biomolecules in plants has led to the development of a variety of in vivo analytical and extraction methods. Compared with other sampling techniques like microdialysis, in vivo tissue collection and biosensors, SPME offers the advantage of a low cost and simple device [44,71,[73][74][75]. This technique allows the efficient and non-exhaustive extraction of the analytes by inserting the fiber directly in plant tissues with minimum disturbance of the living system.…”

Section: In Vivo Spme Extraction Of Plant Tissuesmentioning

confidence: 99%

“…Along with xenobiotics, phytohormones and carbohydrates represent other two important classes of analytes of biological interest. Such endogenous compounds are involved in a variety of regulation and pathological processes [72,75].…”

Section: In Vivo Spme Extraction Of Plant Tissuesmentioning

confidence: 99%

Recent Advances in In Vivo SPME Sampling

et al. 2020

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In vivo solid-phase microextraction (SPME) has been recently proposed for the extraction, clean-up and preconcentration of analytes of biological and clinical concern. Bioanalysis can be performed by sampling exo- or endogenous compounds directly in living organisms with minimum invasiveness. In this context, innovative and miniaturized devices characterized by both commercial and lab-made coatings for in vivo SPME tissue sampling have been proposed, thus assessing the feasibility of this technique for biomarker discovery, metabolomics studies or for evaluating the environmental conditions to which organisms can be exposed. Finally, the possibility of directly interfacing SPME to mass spectrometers represents a valuable tool for the rapid quali- and quantitative analysis of complex matrices. This review article provides a survey of in vivo SPME applications focusing on the extraction of tissues, cells and simple organisms. This survey will attempt to cover the state-of- the-art from 2014 up to 2019.

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A novel probe based on phenylboronic acid functionalized carbon nanotubes for ultrasensitive carbohydrate determination in biofluids and semi-solid biotissues

Abstract: An ultrasensitive SPME probe based on phenylboronic acid functionalized CNTs is applied for direct in vitro or in vivo recognition of carbohydrates in biofluids as well as semi-solid biotissues.

Cited by 65 publications

References 47 publications

Allochroic‐Graphene Oxide Linked 3D Oriented Surface Imprinting Strategy for Glycoproteins Assays

Allochroic‐Graphene Oxide Linked 3D Oriented Surface Imprinting Strategy for Glycoproteins Assays

Boronate Affinity–Molecularly Imprinted Biocompatible Probe: An Alternative for Specific Glucose Monitoring

Recent Advances in In Vivo SPME Sampling

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