A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications

Uzun, Sema Demirci; Ünlü, Naime Akbaşoğlu; Sendur, Merve; Kanik, Fulya Ekiz; Tımur, Suna; Toppare, Levent

doi:10.1016/j.colsurfb.2013.07.049

Cited by 35 publications

(12 citation statements)

References 43 publications

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“…XPS spectra of PPP‐NH 2 ‐ g‐ PEG polymer (Figure A,C) and PPP‐NH 2 ‐ g‐ PEG/DOX bioconjugate (Figure B,D) were decomposed in peak components that can be assigned to different characteristic chemical species. PPP‐NH 2 ‐ g‐ PEG polymer exhibits signals related to the alkyl carbons (284.1 eV), C–O/C–N groups (286.0 eV) and characteristic –COOR group (290.7 eV) because of the PEG modified polymer structure (Figure A) . Following bioconjugation of DOX to the PPP‐NH 2 ‐ g‐ PEG polymer, there appears an additional peak at 285.8 eV corresponding to the C in the C=N bonds of hydrazone (Figure B) .…”

Section: Resultsmentioning

confidence: 99%

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Guler

Akbulut

Barlas

et al. 2016

Macromolecular Bioscience

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Conventional cancer treatments such as chemotherapy, radiotherapy, or combination of these two result in side effects, which lower the quality of life of the patients. To overcome problems with these methods, altering the drug properties by conjugating them to carrier polymers has emerged. Such polymeric carriers also hold the potential to make tumor cells more sensitive to radiation therapy. Herein, poly(p-phenylene) (PPP) polymer with poly(ethylene glycol) (PEG) chains and primary amino groups (PPP-NH2 -g-PEG) is synthesized and conjugated with anticancer drug Doxorubicin (DOX). pH dependent drug release experiments are performed at pH 5.3 and pH 7.4, respectively. Cell viability studies on human cervix adenocarcinoma cells show that lower doses of DOX inhibit cell proliferation when conjugated with nontoxic doses of PPP-NH2 -g-PEG polymer. Additionally, PPP-NH2 -g-PEG/Cys/DOX bioconjugate significantly increases radiosensitive properties of DOX. It is possible to use lower doses of DOX when conjugated to PPP-NH2 -g-PEG in combination with radiotherapy.

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Section: Resultsmentioning

confidence: 99%

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Guler

Akbulut

Barlas

et al. 2016

Macromolecular Bioscience

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“…Due to the presence of aromatic units in the polymer backbone, the immobilization is implemented with the π–π stacking interactions of the polymer and enzyme. These potent interactions allow to maintain the tertiary structure of proteins 11. The electrochemical nature of the conducting polymer was used to amplify the electron transfer during amperometric measurements; besides, the stability, durability and sensitivity of the biosensor were enhanced with the use of semiconducting structures 12.…”

Section: Resultsmentioning

confidence: 99%

Tyrosinase Biosensor for Antioxidants Based on Semiconducting Polymer Support

et al. 2016

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This study reports sensitive phenolic compounds detection using biosensing electrode constructed by immobilization of tyrosinase in an electrochemically synthesized copolymer based on N‐nonylcarbazole derivatives on a platinum (Pt) electrode. Tyrosinase has been successfully immobilized (electrolytic deposition) on the surface of thin film built of poly[2,7‐bis(selenophene)‐N‐nonylcarbazole] and poly[3,6‐bis(selenophene)‐N‐nonylcarbazole]. A well‐defined reduction current according to the phenolic compounds was observed in cyclic voltammetry, which assigned to the reduction of biocatalytically produced o‐quinones on the electrode surface. The immersion of the tyrosinase‐equipped electrode in solution with substrate generated large catalytic currents easily recorded by cyclic voltammetry. The response of the biosensing arrangement was estimated in the presence of catechol and L‐3,4‐dihydroxyphenylalanine (L‐DOPA). The system exhibits an explicit catalytic activity and the substrates can be amperometrically determined at +0.07 V vs. Ag/AgCl. The activation energy (Ea) of immobilized tyrosinase catalytic reaction was estimated as 24.65 kJ/mol in PBS buffer. The analytical properties of the developed biosensor, such as linear concentration range, sensitivity, detection limit and reproducibility, repeatibility were also evaluated. Considering the fact, that the immobilization policy proved high efficiency, the results suggest that the method for phenoloxidase immobilization has a big capacity of providing high throughput engineering of bioelectronic devices.

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“…A c c e p t e d M a n u s c r i p t groups of polymer and biomolecules [19]. Various techniques can be used for the enzyme immobilization such as physical adsorption, electrochemical immobilization, entrapment, covalent binding, etc.…”

Section: Page 11 Of 27mentioning

confidence: 99%

“…Then, 2 µL ChOx (0.24 U) was immobilized with the help of gluteraldehyde (2 µL, 1 %) on the polymer coated graphite electrode and dried at room temperature for 2 hours. Gluteraldehyde is a well known crosslinking agent to stabilize the biomolecule on the surface [19]. After that, the poly (CBNP)/ChOx/graphite electrode was stored at +4 ºC for 3 hours to obtain amperometric responses.…”

Section: Preparation Of the Poly (Cbnp)/chox Sensormentioning

confidence: 99%

Electrochemical and optical properties of a conducting polymer and its use in a novel biosensor for the detection of cholesterol

Söylemez

Udum

Kesik

et al. 2015

Sensors and Actuators B: Chemical

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A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications

Cited by 35 publications

References 43 publications

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Tyrosinase Biosensor for Antioxidants Based on Semiconducting Polymer Support

Electrochemical and optical properties of a conducting polymer and its use in a novel biosensor for the detection of cholesterol

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