A Novel Pyroptosis-related Gene Signature for Prognostic Prediction of Head and Neck Squamous Cell Carcinoma

Qian, Xiaoyu; Tang, Jian; Chu, Yongquan; Chen, Ziqiang; Chen, Liang; Shen, Chao; Li, Lin

doi:10.21203/rs.3.rs-620847/v1

Cited by 5 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16 Latest two studies had reported the prognostic prediction of pyroptosis signature in HNSCC. 36,37 They identified both few PRGs with a 4- To accurately evaluate the pyroptosis features in each HNSCC patient, we constructed a pyroptosis score system using the PCA algorithm. We found that patients with low pyroptosis scores usually presented shorter survival times and higher death rates, suggesting that low pyroptosis scores might predict poor outcomes in HNSCC.…”

Section: Discussionmentioning

confidence: 99%

“…Several lines of evidence indicated that pyroptosis plays a crucial role in regulating antitumor immunity and inflammation 16 . Latest two studies had reported the prognostic prediction of pyroptosis signature in HNSCC 36,37 . They identified both few PRGs with a 4‐gene and a 5‐gene signature, respectively, and analyzed the immune cell infiltration of the risk model simply.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pyroptosis patterns and immune infiltrates characterization in head and neck squamous cell carcinoma

Deng

Wei

Qiu

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background: Pyroptosis plays an essential role in tumor immune responses and inflammation related to chemotherapy. Herein, we studied the characteristic patterns of pyroptosis in head and neck squamous cell carcinoma (HNSCC) to determine their prognostic and therapeutic effects. Methods: Consensus clustering analysis was performed to classify patients into pyroptosis or gene clusters. A novel pyroptosis score was constructed by principal component analysis. Kaplan-Meier survival curves were used to show the prognostic value. We also assessed the functional enrichment, tumor mutation burden, immune cell infiltration, and the sensitivity to chemotherapy and immunotherapy between high and low pyroptosis score group. Results: Two distinct pyroptosis clusters were defined based on the mRNA expression profiles of PRGs, which were related to immune activation in HNSCC. Notably, a pyroptosis score was constructed according to different expression gene signatures, and then, each HNSCC patient was classified into a low or high pyroptosis score group. Patients with low pyroptosis scores had better immunotherapeutic responses and higher sensitivities to chemotherapeutic agents (paclitaxel, docetaxel, and gemcitabine). Kaplan-Meier survival curves showed that the pyroptosis patterns were independent prognostic indicators regardless of the level of tumor mutation burden. Conclusions: Pyroptosis plays an essential role in immune infiltration in HNSCC. Quantifying the pyroptosis score of individual patients might suggest prognostic, immunotherapeutic, and chemotherapeutic strategies for HNSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pyroptosis patterns and immune infiltrates characterization in head and neck squamous cell carcinoma

Deng

Wei

Qiu

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…Chen et al [14] constructed eight-PRG signature (AIM2, BAK1, GZMA, GZMB, IRF1, NOD2, TNF, and TP63) to predict the survival in bladder cancer (BC) patients. Qian et al [15] performed a novel PRG signature (GSDME, IL1B, NLRP1, and NLRP6) for prognostic prediction of head and neck squamous cell carcinoma. In EC, Chen et al [23] recently analyzed 33 PRGs between tumour samples and normal samples and obtained six pyroptosisrelated prognostic DEGs (GPX4, GSDMD, GSDME, IL6, NOD2, and PYCARD).…”

Section: Discussionmentioning

confidence: 99%

“…For example, the expression of pyroptotic inflammasome GSDMD is decreased in gastric cancer (GC) cells, which promotes the proliferation of GC cells [10]. Recent studies have shown that PRG signatures can be used as effective prognostic markers in ovarian cancer [12], lung cancer [13], bladder cancer [14], and squamous cell cancer in the head and neck [15]. However, the prognostic value of PRGs in EC has not yet been elucidated.…”

Section: Introductionmentioning

confidence: 99%

A Novel Defined Pyroptosis-Related Gene Signature for Predicting the Prognosis of Endometrial Cancer

Liu

Zeng

et al. 2022

Disease Markers

View full text Add to dashboard Cite

Endometrial carcinoma (EC) is the second major female genital malignancy. Genetic signatures may be an improved choice to predict the prognosis of EC patients. The relationship between pyroptosis and tumours has attracted much attention in recent years. Here, we constructed a new pyroptosis-related gene (PRG) signature for predicting the prognosis of EC. In this study, gene data and clinical information of EC patients were obtained from The Cancer Genome Atlas (TCGA). Following the identification of PRGs correlated with EC prognosis, we further investigate the bioinformatics functions of these PRGs by univariate Cox regression analysis and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Then, we used the least absolute contraction and selection operator (LASSO) regression and multiple Cox regression analysis to construct a new PRG signature that contains seven PRGs (NFKB1, EEF2K, CTSV, MDM2, GZMB, PANX1, and PTEN) and performed the Kaplan-Meier (K-M) analysis, receiver operating characteristic curve (ROC) analysis, and principal component analysis (PCA) to evaluate the prognostic value of our novel PRG signature. Finally, we assessed the correlations between pyroptosis and immune cells/checkpoints through the CIBERSORT tool and single-sample gene set enrichment analysis (ssGSEA). The result suggested that our signature was powerful in predicting EC prognosis and may play a part in assessing response to immunotherapy in EC patients. In conclusion, our study established a novel PRG signature for EC, which can be used as an effective prognostic marker in clinical practice in the future.

show abstract

“…In total, 52 PRGs were extracted from prior publications [14][15][16][17], previous pyroptosis-related studies [18][19][20][21][22][23][24][25][26][27][28][29][30][31] and MSigDB database (v7.4), [32], respectively (Supplementary Table S1). The expression data in all the three datasets were normalized to fragment per kilobase million (FPKM) values before comparison.…”

Section: Identification Of Differentially Expressed Pyroptosis Relate...mentioning

confidence: 99%

Pyroptosis related genes signature predicts prognosis and immune infiltration of tumor microenvironment in hepatocellular carcinoma

et al. 2022

View full text Add to dashboard Cite

Little is known on the relationship between the expression of pyroptosis related genes (PRGs) and prognosis of hepatocellular carcinoma (HCC). In this study, a specific PRGs prognostic model was developed with an aim to improve therapeutic efficiency among HCC patients. In total, 42 PRGs that were differentially expressed between HCC tissues and adjacent tissues and we exhibited the mutation frequency, classification, the location of copy number variation (CNV) alteration and the CNV variation frequency of PRGs. Two clusters were distinguished by the consensus clustering analysis based on the 42 differentially expressed genes (DEGs). There were significant differences in clinical features including T stage, grade, gender, and stage among different clusters. Kaplan–Meier curve analysis showed that cluster 1 had a better prognosis than cluster 2. The prognostic value of PRGs for survival was evaluated to construct a multigene signature using The Cancer Genome Atlas (TCGA) cohort. Based on the univariate analysis and multivariate analysis, a 10-gene signature was built and all HCC patients in the TCGA cohort were divided into low-risk group and high-risk group. HCC patients in the high-risk group showed significantly lower survival possibilities than those in the low-risk group (P < 0.001). Utilizing the median risk score from the TCGA cohort, HCC patients from International Cancer Genome Consortium (ICGC)-LIRI-JP cohort and Gene Expression Omnibus (GEO) cohort (GSE14520) were divided into two risk subgroups. The result showed that overall survival (OS) time was decreased in the high-risk group. Combined with the clinical characteristics, the risk score was an independent factor for predicting the OS of HCC patients. Then, ROC curve and survival analysis were performed to evaluate the prognostic prediction value of the model. Finally, we constructed a PRGs clinical characteristics nomogram to further predict HCC patient survival probability. There were significant differences in immune cell infiltration, GSEA enrichment pathway, IC50 of chemotherapeutics, PRGs mutation frequency between high-risk group and low-risk group. This work suggests PRGs signature played a crucial role in predicting the prognosis, infiltration of cancer microenvironment, and sensitivity of chemotherapeutic agents.

show abstract

A Novel Pyroptosis-related Gene Signature for Prognostic Prediction of Head and Neck Squamous Cell Carcinoma

Cited by 5 publications

References 22 publications

Pyroptosis patterns and immune infiltrates characterization in head and neck squamous cell carcinoma

Pyroptosis patterns and immune infiltrates characterization in head and neck squamous cell carcinoma

A Novel Defined Pyroptosis-Related Gene Signature for Predicting the Prognosis of Endometrial Cancer

Pyroptosis related genes signature predicts prognosis and immune infiltration of tumor microenvironment in hepatocellular carcinoma

Contact Info

Product

Resources

About