A Novel R2R3-MYB Transcription Factor Contributes to Petal Blotch Formation by Regulating Organ-Specific Expression of<i>PsCHS</i>in Tree Peony (<i>Paeonia suffruticosa</i>)

Gu, Zhennan; Zhu, Jianzhong; Hao, Qing; Yuan, Yao‐Wu; Duan, Yuan‐Wen; Men, Siqi; Wang, Qianyu; Hou, Qinzheng; Liu, Zheng-An; Shu, Qingyan; Wang, Liang-Sheng

doi:10.1093/pcp/pcy232

Cited by 96 publications

(86 citation statements)

References 72 publications

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“…Co-expression network revealed PlFLS is the most likely target gene interacting with PlMYB10. Previous studies have verified that R2R3-MYB can regulate the expression pattern of FLS through the overexpression of PsMYB114L (from Paeonia suffruticosa) in Arabidopsis [49], which is consistent with our results. Thus, we tentatively speculate about the ABP of P. limprichtii (Figure 9).…”

Section: Discussionsupporting

confidence: 93%

Comparative Transcriptomics Provides Insight into Floral Color Polymorphism in a Pleione limprichtii Orchid Population

Zhang

Zhou²,

Dai

et al. 2019

IJMS

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Floral color polymorphism can provide great insight into species evolution from a genetic and ecological standpoint. Color variations between species are often mediated by pollinators and are fixed characteristics, indicating their relevance to adaptive evolution, especially between plants within a single population or between similar species. The orchid genus Pleione has a wide variety of flower colors, from violet, rose-purple, pink, to white, but their color formation and its evolutionary mechanism are unclear. Here, we selected the P. limprichtii population in Huanglong, Sichuan Province, China, which displayed three color variations: Rose-purple, pink, and white, providing ideal material for exploring color variations with regard to species evolution. We investigated the distribution pattern of the different color morphs. The ratio of rose-purple:pink:white-flowered individuals was close to 6:3:1. We inferred that the distribution pattern may serve as a reproductive strategy to maintain the population size. Metabolome analysis was used to reveal that cyanindin derivatives and delphidin are the main color pigments involved. RNA sequencing was used to characterize anthocyanin biosynthetic pathway-related genes and reveal different color formation pathways and transcription factors in order to identify differentially-expressed genes and explore their relationship with color formation. In addition, qRT-PCR was used to validate the expression patterns of some of the genes. The results show that PlFLS serves as a crucial gene that contributes to white color formation and that PlANS and PlUFGT are related to the accumulation of anthocyanin which is responsible for color intensity, especially in pigmented flowers. Phylogenetic and co-expression analyses also identified a R2R3-MYB gene PlMYB10, which is predicted to combine with PlbHLH20 or PlbHLH26 along with PlWD40-1 to form an MBW protein complex (MYB, bHLH, and WDR) that regulates PlFLS expression and may serve as a repressor of anthocyanin accumulation-controlled color variations. Our results not only explain the molecular mechanism of color variation in P. limprichtii, but also contribute to the exploration of a flower color evolutionary model in Pleione, as well as other flowering plants.

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Section: Discussionsupporting

confidence: 93%

Comparative Transcriptomics Provides Insight into Floral Color Polymorphism in a Pleione limprichtii Orchid Population

Zhang

Zhou²,

Dai

et al. 2019

IJMS

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“…In a recent study, Gu et al suggested that anthocyanin accumulation occurred 10 d before anthesis in tree peony 'Qing Hai Hu Yin Bo' [47]. Here, we found anthocyanins were up-regulated after endodormancy release, accumulating in large amounts at ecodormancy stage in tree peony 'Luhehong' (Fig.…”

Section: Flavonoids Accumulation At Ecodormancy Stagesupporting

confidence: 58%

Metabonomic analysis reveals EMP pathway activation and flavonoid accumulation during dormancy transition in tree peony

Zhang

Yuan

Zhan

et al. 2020

Preprint

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Background Bud dormancy is a sophisticated strategy in which plants evolved to survive in tough environments. Chilling exposure is an effective method to promote dormancy release in perennial plants including tree peony, and endodormancy is a key obstacle for forcing culture of tree peony. However, the mechanism of dormancy release is still poorly understood, and there are few systematic studies on the metabolomics during chilling induced dormancy transition. Results A total of 535 small molecules were obtained in tree peony buds treated with chilling, belonging to flavonoids, amino acid and its derivatives, lipids, organic acids and its derivates, nucleotide and its derivates, alkaloids, hydroxycinnamoyl derivatives, carbohydrates and alcohols, phytohormones, coumarins, and vitamins, respectively. 118 differential metabolites were detected, and they involved in serveral metabolic pathways related to dormancy. Sucrose was the most abundant carbohydrate in peony bud. Starch degradation and EMP activity were enhanced during the dormancy release, according to the variations of sugar contents, related enzyme activities and key genes expression. Flavonoids synthesis and accunmulation were also promoted by prolonged chilling, which might attribute to the activated expression of PsDFR and PsANS. Moreover, the variations of phytohormones (SA, JA, ABA, and IAA) indicated they played different roles in dormancy transition. Conclusion Our study suggested that starch degradation, EMP activation, and flavonoid accumulation were crucial and associated with bud dormancy transition in tree peony.

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“…The anthocyanin biosynthesis pathway (ABP) is highly conserved and has been well studied in plants 6 . As crucial ABP regulators, the R2R3-MYB transcription factors can regulate the expression of structural genes to influence flower colour formation [8][9][10][11][12][13] . In Orchidaceae, some R2R3-MYBs have been verified to activate ABP structural genes to promote anthocyanin accumulation in Phalaenopsis 5 , Oncidium 14 and Dendrobium 15 flowers.…”

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confidence: 99%

New insight into the molecular mechanism of colour differentiation among floral segments in orchids

Zheng

Wang

et al. 2020

Commun Biol

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An unbalanced pigment distribution among the sepal and petal segments results in various colour patterns of orchid flowers. Here, we explored this type of mechanism of colour pattern formation in flowers of the Cattleya hybrid 'KOVA'. Our study showed that pigment accumulation displayed obvious spatiotemporal specificity in the flowers and was likely regulated by three R2R3-MYB transcription factors. Before flowering, RcPAP1 was specifically expressed in the epichile to activate the anthocyanin biosynthesis pathway, which caused substantial cyanin accumulation and resulted in a purple-red colour. After flowering, the expression of RcPAP2 resulted in a low level of cyanin accumulation in the perianths and a pale pink colour, whereas RcPCP1 was expressed only in the hypochile, where it promoted α-carotene and lutein accumulation and resulted in a yellow colour. Additionally, we propose that the spatiotemporal expression of different combinations of AP3and AGL6-like genes might participate in KOVA flower colour pattern formation.

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A Novel R2R3-MYB Transcription Factor Contributes to Petal Blotch Formation by Regulating Organ-Specific Expression ofPsCHSin Tree Peony (Paeonia suffruticosa)

Cited by 96 publications

References 72 publications

Comparative Transcriptomics Provides Insight into Floral Color Polymorphism in a Pleione limprichtii Orchid Population

Comparative Transcriptomics Provides Insight into Floral Color Polymorphism in a Pleione limprichtii Orchid Population

Metabonomic analysis reveals EMP pathway activation and flavonoid accumulation during dormancy transition in tree peony

New insight into the molecular mechanism of colour differentiation among floral segments in orchids

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