A novel receptor for secretory component on porcine mononuclear cells

Setcavage, Thomas M.; Rothlein, Robert; Muscoplat, Charles C; Kim, Yoon B.

doi:10.1016/0008-8749(76)90152-0

Cited by 13 publications

(3 citation statements)

References 12 publications

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“…It can be assumed that the majority cor responded to immature T cells, able to form E-RFC when fixed with glutaraldehyde [25], On the other hand, part of these cells may bear receptors for the secretory piece of IgA [31]. Interestingly no null cells were encountered in LL from Er,-RFC depletion, contrary to the two other types of RFC depletion.…”

Section: Discussionmentioning

confidence: 68%

Surface Markers of Porcine Lymphocytes and Distribution in Various Lymphoid Organs

Salmon¹

1979

Int Arch Allergy Immunol

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Three rosette test systems were developed for the classification of subpopulations of porcine lymphocytes and correlated with the presence or the absence of membrane immunoglobulins, as well as agg-P-IgG receptors, by means of various RFC depletion experiments. Among the E_D-RFC negative cells, PBL with surface immunoglobulins (SIg), at 4°C, can be subdivided into two about equal populations: (1) One half of these cells have surface stable SIg (B cells) and μ determinants; they possess C₃b receptors leading to the formation of rosettes with complement-coated zymosan particles (ZC). (2) The other half have surface labile Ig when the cells are washed at 37°C (L-cells); they possess Fc receptors as detected by EA-RFC, i. e. pig-IgG-coated ox RBC. Receptors for Fc-aggas detected by agg-P-IgG, were primarily shown on B cells. ZC and EA-RFC are in the highest tissue concentration, in bone marrow and spleen, respectively, and more numerous in adults than in newborns. Moreover, the number of EA-RFC was twofold higher than that of ZC-RFC in spleen, mesenteric lymph node and cistern a chyli. This latter organ was the only one without null cell.

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Section: Discussionmentioning

confidence: 68%

Surface Markers of Porcine Lymphocytes and Distribution in Various Lymphoid Organs

Salmon¹

1979

Int Arch Allergy Immunol

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“…Distinct receptors for SC have been reported on rabbit and porcine lympho cytes (114,126), although such receptors have not been detected on human lymphoid cells (12,15). The data for the presence of such receptors is not convincing, and the possibility has not been excluded that the SC preparations used to detect the receptor contained a small fragment of the a chain that inserted into the FcaR and inhibited the formation of rosettes with IgA-coated erythrocytes.…”

Section: Specificity and Binding Parameters Of Fcarmentioning

confidence: 96%

Regulation of IgA Expression by Isotype-Specific T Cells and Soluble Binding Factors

Word¹,

Crago²,

Tomasi³

1986

Annu. Rev. Microbiol.

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The data presented here suggest a model for isotype-specific regulation of IgA synthesis by Fc alpha R+ T cells (Figure 1). Immature mIgM+ +/- mIgD+ B cells are induced by T switch cells to express cell surface IgA (a phenotypic switch). If the T switch cell induces mIgA expression via a long primary RNA transcript from an unrearranged C alpha allele, the hypothetical intermediate switch B cell results (step 1); this may be the mechanism of heavy chain expression in memory B cells that express low levels of Ig. Alternatively, T switch cells may induce a DNA rearrangement in the CH locus of the B cell (a genotypic switch), which results in a deletion of all CH loci except C alpha (step 2). TH inducer cells promote maturation of mIgA+ B cells to IgA-secreting plasma cells. This may involve a DNA switch rearrangement (step 3) or the maturation of previously switched cells (step 4), and appears to be mediated via an IgABF with enhancing activity. Not shown in this figure, but inherent in this model, is a suppressive regulatory arm that may be mediated via IgABF with suppressive activity released from Fc alpha R+ suppressor T cells. Due to the presence of Fc alpha R on a variety of cell types, IgABF may suppress synthesis of IgA by acting not only on mIgA+ B cells but also on regulatory cells (T cells, B cells, and macrophages) bearing IgA bound to Fc alpha R. If the IgA system is analogous with the IgE system, mIgA-bearing B cells may be the direct target of IgABF. Binding of Ig to FcR has been shown to (a) increase the number of Fc receptors per cell, (b) enhance the number of cells expressing Fc receptors, (c) induce the release of IgBF that either suppress or enhance Ig secretion, and (d) effectively convert surface Ig- cells into surface Ig+ cells that are therefore receptive to IgBF. Thus, FcR+ cells may interact with IgBF and Ig via a regulatory network to stimulate or inhibit the immune response in an isotype-specific manner. Cell surface molecules (mIg, FcR) may serve as sensors that allow the cell to detect and respond to fluctuations in the levels of immune mediators that serve to modulate Ig synthesis and secretion. The relationship between IgBF and FcR is not known, nor is it known whether Fc receptors expressed by different cell types are encoded by the same gene and are controlled similarly.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Cells were stained as previously described [ 14], Briefly, 2 X 106 cells were placed in 1-ml centri fuge tubes and washed twice with a phosphatebuffered saline containing 2%i bovine serum albu min and 0.1 sodium azide (PBS-BSA-N3). After the second wash, the cells were suspended in 0.05 ml of PBS-BSA-N, and an equal volume of FITC-conjugated rabbit antibovine lgG antiserum with both heavy and light chain activity (Cappel, Cochranville, Pa.).…”

Section: Fluorescent Antibody Staining On Live Cellsmentioning

confidence: 99%

Enumeration and Characterization of Bovine Blood, Spleen and Lymph Node Cells Containing Immunoglobulins

Rothlein¹,

Johnson²,

Muscoplat³

1980

Int Arch Allergy Immunol

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In this study, the average percentage of bovine spleen and lymph node cells with surface immunoglobulin (S-Ig) was found to be 19.62 and 23.18%, respectively. The average percentage of these cells with cytoplasmic immunoglobulin (C-Ig) was 14.46 and 17.21%. Only the percentage of cells with S-Ig showed a strong correlation between the spleen and the lymph node. Also, several methods of removing S-Ig from bovine peripheral blood mononuclear cells were investigated. It was found that the loss of passively bound S-Ig by warm washing was minimal, and that the anti-Ig-treated mononuclear blood cells would lose their caps after 24 h of incubation at 37 °C, but not after 45 min of incubation, although 95% of the cells with S-Ig were capped within 15 min.

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A novel receptor for secretory component on porcine mononuclear cells

Cited by 13 publications

References 12 publications

Surface Markers of Porcine Lymphocytes and Distribution in Various Lymphoid Organs

Surface Markers of Porcine Lymphocytes and Distribution in Various Lymphoid Organs

Regulation of IgA Expression by Isotype-Specific T Cells and Soluble Binding Factors

Enumeration and Characterization of Bovine Blood, Spleen and Lymph Node Cells Containing Immunoglobulins

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