A novel recombinantly produced banana lectin isoform is a valuable tool for glycoproteomics and a potent modulator of the proliferation response in CD3+, CD4+, and CD8+ populations of human PBMCs

In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma

“…BanLec has been recognised as a potent IgG4-inducing antigen (Ag) [16], as a T cell mitogen [8,9,17] and as a stimulator of nitric oxide (NO) production by peritoneal macrophages [18]. Its resistance to the proteolytic enzymes of the gastrointestinal tract [8] enables BanLec to pass through the mucosal barrier and to interact with the immune system by inducing a strong IgG4 immune response.…”

The Amaranthus leucocarpus Lectin Enhances the Anti-CD3 Antibody-Mediated Activation of Human Peripheral Blood CD4+ T Cells

“…Lectins are glycoproteins found in plants that recognize cell surface oligosaccharides and have been used as tools in immunology to characterize changes to oligosaccharides present on T cells and their effects on T cell activation (Galvan et al 1998;Sharon and Lis 2004;Merant et al 2005;Shanmugham et al 2006;Kesherwani and Sodhi 2007;Gavrovic-Jankulovic et al 2008). Moreover, some lectins can activate T cells by cross-linking T cell surface r e c e p t o r s i n a c a r b o h y d r a t e -d e p e n d e n t m a n n e r (Shanmugham et al 2006;Kesherwani and Sodhi 2007).…”

Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy

“…Presence of carbohydrate residues in the Act d 4 molecule was examined by two mannose-binding lectins, Concanavalin A (Con A) and recombinant BanLec (rBanLec), and fucose-binding Aleuria aurantia lectin (AAL) according to the previously described procedure [32]. In brief, 0.5 mg/mm of Act d 4 protein was loaded onto the SDS-gel.…”