1989
DOI: 10.1099/0022-1317-70-5-1105
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A Novel Replicative Form DNA of Aleutian Disease Virus: the Covalently Closed Linear DNA of the Parvoviruses

Abstract: SUMMARYThe analysis of replicative form (RF) DNA of Aleutian disease virus (ADV) by alkaline gel electrophoresis revealed that all RF DNA species segregate into DNA single strands which represent integral multiples of a genome equivalent. This" demonstrates that as with other autonomous parvoviruses, the virion and complementary DNA strands are frequently linked by hairpin structures and that also, nicks are present at subterminal sites. Approximately 50 ~ of the Y-terminal hairpins contain a subterminal nick … Show more

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Cited by 12 publications
(6 citation statements)
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“…Thus, the cellular extract appears to have little capacity, by itself, to unfold and copy the right-end genomic hairpin which becomes ligated to the growing complementary strand. The cRF species was recently detected in cells infected with the autonomous parvoviruses MVM (13) and Aleutian disease virus (35) and was postulated to be an early intermediate in parvovirus DNA replication (17,56). Our data argue for this possibility by showing that cRF DNA is the direct product of the conversion reaction taking place under in vitro conditions.…”
Section: Discussionsupporting
confidence: 61%
See 1 more Smart Citation
“…Thus, the cellular extract appears to have little capacity, by itself, to unfold and copy the right-end genomic hairpin which becomes ligated to the growing complementary strand. The cRF species was recently detected in cells infected with the autonomous parvoviruses MVM (13) and Aleutian disease virus (35) and was postulated to be an early intermediate in parvovirus DNA replication (17,56). Our data argue for this possibility by showing that cRF DNA is the direct product of the conversion reaction taking place under in vitro conditions.…”
Section: Discussionsupporting
confidence: 61%
“…In vitro conversion of ss virion DNA into ds RF DNA was achieved with partially purified eukaryotic DNA polymerases alpha (24) and gamma (29), as well as with the large fragment of Escherichia coli DNA polymerase I (7,45). Unit length open RF (oRF) and cRF DNAs, as well as concatemers of dimer and tetramer length were detected in parvovirus-infected cells (13,35,54). Resolution of left-to-left-end, as well as right-to-right-end, junction fragments of MVM dimeric and tetrameric RF DNAs was reported to occur in vivo and in vitro and was shown to require the nicking activity of the MVM NS1 protein (14,15,20,21,34).…”
mentioning
confidence: 99%
“…Abbreviations are defined in the text. molecule identified and characterized recently (8,19). The presence of this form in the eluate (lane 2) confirms the binding of the hairpinned 3' terminus to VP1.…”
Section: Figsupporting
confidence: 58%
“…This results in a molecule covalently closed at both ends (cRF) (14). Such closed forms were also detected in parvovirus-infected cells (21,22). Further processing of cRF DNA in vitro requires the MVM nonstructural protein NS1 (14, 15).…”
mentioning
confidence: 91%