A novel restorative pulmonary valved conduit in a chronic sheep model: Mid-term hemodynamic function and histologic assessment

Bennink, Ger B.W.E.; Torii, Sho; Brugmans, Marieke; Cox, Martijn; Svanidze, Oleg; Ladich, Elena; Carrel, Thierry; Virmani, Renu

doi:10.1016/j.jtcvs.2017.12.046

Cited by 86 publications

(85 citation statements)

References 19 publications

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“…22 PCL-BU was chosen as the biomaterial of interest as it belongs to a class of elastomeric thermoplastic materials that is susceptible to enzymatic and oxidative degradation 11 and has demonstrated its potential for in situ cardiovascular tissue engineering applications. [30][31][32][33][34] Secondly, human THP-1 derived monocytes were seeded in comparable scaffolds (using fibrin as a cell carrier), stimulated to become macrophages and exposed to physiological loading conditions (shear stress, cyclic stretch or a combination thereof ) for 8 days in the bioreactor. Statically cultured macrophage-seeded tubular scaffolds were included as experimental controls.…”

Section: Experimental Outlinementioning

confidence: 99%

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

et al. 2020

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Section: Experimental Outlinementioning

confidence: 99%

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

et al. 2020

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“…In general, supramolecular biomaterials show a relative ease of processing and provide the possibility to finely tune the morphological, chemical, and thereby, degradation properties (Bouten et al, 2011; Brugmans et al, 2015; van Almen et al, 2016). Accordingly, these materials are being tested with regard to in situ tissue engineering for cardiovascular applications (Muylaert et al, 2015; van Almen et al, 2016; Bockeria et al, 2017; Kluin et al, 2017; Bennink et al, 2018). PCL-BU scaffolds, specifically, have been tested as vascular grafts, revealing relatively rapid degradation kinetics in vivo (Duijvelshoff et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Macrophage-Driven Biomaterial Degradation Depends on Scaffold Microarchitecture

Wissing

Bonito

Haaften

et al. 2019

Front. Bioeng. Biotechnol.

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In situ tissue engineering is a technology in which non-cellular biomaterial scaffolds are implanted in order to induce local regeneration of replaced or damaged tissues. Degradable synthetic electrospun scaffolds are a versatile and promising class of biomaterials for various in situ tissue engineering applications, such as cardiovascular replacements. Functional in situ tissue regeneration depends on the balance between endogenous neo-tissue formation and scaffold degradation. Both these processes are driven by macrophages. Upon invasion into a scaffold, macrophages secrete reactive oxygen species (ROS) and hydrolytic enzymes, contributing to oxidative and enzymatic biomaterial degradation, respectively. This study aims to elucidate the effect of scaffold microarchitecture, i.e., μm-range fiber diameter and fiber alignment, on early macrophage-driven scaffold degradation. Electrospun poly-ε-caprolactone-bisurea (PCL-BU) scaffolds with either 2 or 6 μm (Ø) isotropic or anisotropic fibers were seeded with THP-1 derived human macrophages and cultured in vitro for 4 or 8 days. Our results revealed that macroph age-induced oxidative degradation in particular was dependent on scaffold microarchitecture, with the highest level of ROS-induced lipid peroxidation, NADPH oxidase gene expression and degradation in the 6 μm Ø anisotropic group. Whereas, biochemically polarized macrophages demonstrated a phenotype-specific degradative potential, the observed differences in macrophage degradative potential instigated by the scaffold microarchitecture could not be attributed to either distinct M1 or M2 polarization. This suggests that the scaffold microarchitecture uniquely affects macrophage-driven degradation. These findings emphasize the importance of considering the scaffold microarchitecture in the design of scaffolds for in situ tissue engineering applications and the tailoring of degradation kinetics thereof.

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“…However, a tissue-engineered leaflet that lack the trilayered-structure of a native leaflet may face major functional problems in long-term valve replacement because long-term efficient leaflet functionalities depend on the appropriate structure of a leaflet [15,19,20]. In previous research studies, most of tissue-engineered valve leaflets ended up with shrinkage either during tissue engineering or after implantation in an in-vivo model although they were capable to bear the physiological load and one of the possible reasons of shrinkage could be lack of native trilayered-structure in the tissue-engineered leaflet as none of them had that required native structure [14,21,22].…”

Section: Introductionmentioning

confidence: 99%

Behavior of valvular interstitial cells on trilayered nanofibrous substrate mimicking morphologies of heart valve leaflet

Jana

Lerman

2019

Acta Biomaterialia

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Heart valve tissue engineering could be an alternative to the current bioprosthetic heart valves and their limitations, especially for pediatric patients. However, heart valve tissue engineering remains challenging because leaflets-the primary component of a heart valve-have three diversely oriented layers-circumferential, random and radial. In order to mimic the orientations, we first designed three novel collectors to fabricate three nanofibrous layers with those orientations from a polymeric biomaterial in an electrospinning system. Then, we devised a novel direct electrospinning technique to develop a unified trilayered nanofibrous (TN) substrate comprising those oriented layers. The TN substrate supported the growth and orientations of seeded porcine valvular interstitial cells (PVICs) and their deposited collagen fibrils. After one month culture, the obtained trilayered tissue construct (TC) exhibited increased tensile properties over its TN substrate. Most importantly, the developed TC did not show any sign of shrinkage. Gene expression pattern of the PVICs indicated the developing stage of the TC. Their protein expression pattern was quite similar to that of leaflets.

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A novel restorative pulmonary valved conduit in a chronic sheep model: Mid-term hemodynamic function and histologic assessment

Abstract: Both conduits demonstrated an acceptable safety and functionality. Significant calcification was rarely observed in the XPV, whereas the H developed more neointimal thickness with calcification of the porcine aortic root portion of the wall.

Cited by 86 publications

References 19 publications

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

Macrophage-Driven Biomaterial Degradation Depends on Scaffold Microarchitecture

Behavior of valvular interstitial cells on trilayered nanofibrous substrate mimicking morphologies of heart valve leaflet

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