A novel ribonuclease with antiproliferative activity toward leukemia and lymphoma cells and HIV-1 reverse transcriptase inhibitory activity from the mushroom, Hohenbuehelia serotina

Abstract

In this study, a 27-kDa ribonuclease (RNase) was purified from the dried fruiting bodies of the mushroom, Hohenbuehelia serotina. The isolation protocol involved anion exchange chromatography, affinity chromatography, cation exchange chromatography and gel filtration in succession. The RNase was unadsorbed on DEAE-cellulose, but was adsorbed on Affi-gel blue gel and CM-cellulose. The N-terminal amino acid sequence was TVGGSLAEKGN which showed homology to other fungal RNases to a certain degree. The RNase exhibited maximal RNase activity at pH 5 and 80˚C. It demonstrated the highest ribonucleolytic activity toward poly(C), a relatively high activity toward poly(U), and a considerably weaker activity toward poly(A) and (G). The RNase inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase with an IC50 of 50 µM and reduced [3H-methyl]-thymidine uptake by L1210 leukemia cells and MBL2 lymphoma cells with an IC50 of 25 µM and 40 µM, respectively.

Lp16-PSP, A Member of YjgF/YER057c/UK114 Protein Family Induces Apoptosis and p21WAF1/CIP1 Mediated G1 Cell Cycle Arrest in Human Acute Promyelocytic Leukemia (APL) HL-60 Cells

“…However, at this stage, it's difficult to conclude the possible molecular mechanism of the action of Lp16-PSP and further support our experimental findings on the basis of previously reported information associated with the other members of YjgF/YER057c/UK114 family.Although antineoplastic, ribonuclease, inhibition of protein synthesis and antiviral activities of the other members of YjgF/YER057c/UK114 protein family has been reported[40][41][42][43][44][45], and afterward it was proven that translation inhibition was driven by endoribonucleolytic activity. In addition, various ribonucleases have also been reported to have selective anticancer properties[46][47][48][49][50]. Based on previously available information related to YjgF/YER057c/UK114 family members and our findings in this study, we believe that Lp16-PSP might have exerted it's in vitro anticancer activity through RNA (s) degradation and/or through the inhibition of protein synthesis.…”

The Chemistry, Pharmacology and Therapeutic Potential of the Edible Mushroom Dictyophora indusiata (Vent ex. Pers.) Fischer (Synn. Phallus indusiatus)

“…Wang and Ng [65] isolated a 28 kDa ribonuclease (RNase) with specific activity of 564 U/mg towards yeast transfer RNA. The potential effect of the RNase in other fields need to be assessed given other RNase from mushrooms such as Hohenbuehelia serotina have been shown to inhibit the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase and inhibit leukemia (L1210) cells and lymphoma (MBL2) cells proliferation [66].…”

Fungal proteinaceous compounds with multiple biological activities

“…Some laccases such as those from P. eryngii (Wang and Ng 2006b), Clitocybe maxima Tricholoma mongolicum (Li et al 2010a) Agaricus placomyces (Sun et al 2012) and Coprinus comatus (Zhao et al 2014), lectins from Russula delica (Zhao et al 2010) Hericium erinaceum (Li et al 2010b), Pholiota adiposa (Zhang et al 2009), Pleurotus citrinopileatus (Li et al 2008) and Lactarius flavidulus (Wu et al 2011), proteases from Cordyceps sobolifera and Xylaria hypoxylon (Hu et al 2012), and ribonucleases from Lactarius flavidulus and Hohenbuehelia serotina (Zhang et al 2014b) inhibited HIV-1 reverse transcriptase activity.…”